Isolation and characterization of Arctic microorganisms decomposing bioplastics
The increasing amount of plastic waste causes significant environmental pollution. In this study, screening of Arctic microorganisms which are able to degrade bioplastics was performed. In total, 313 microorganisms were isolated from 52 soil samples from the Arctic region (Spitsbergen). Among the is...
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ftpubmed:oai:pubmedcentral.nih.gov:5503855 2023-05-15T14:51:10+02:00 Isolation and characterization of Arctic microorganisms decomposing bioplastics Urbanek, Aneta K. Rymowicz, Waldemar Strzelecki, Mateusz C. Kociuba, Waldemar Franczak, Łukasz Mirończuk, Aleksandra M. 2017-07-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503855/ http://www.ncbi.nlm.nih.gov/pubmed/28697585 https://doi.org/10.1186/s13568-017-0448-4 en eng Springer Berlin Heidelberg http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503855/ http://www.ncbi.nlm.nih.gov/pubmed/28697585 http://dx.doi.org/10.1186/s13568-017-0448-4 © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. CC-BY Original Article Text 2017 ftpubmed https://doi.org/10.1186/s13568-017-0448-4 2017-07-30T00:03:52Z The increasing amount of plastic waste causes significant environmental pollution. In this study, screening of Arctic microorganisms which are able to degrade bioplastics was performed. In total, 313 microorganisms were isolated from 52 soil samples from the Arctic region (Spitsbergen). Among the isolated microorganisms, 121 (38.66%) showed biodegradation activity. The ability of clear zone formation on emulsified poly(butylene succinate-co-adipate) (PBSA) was observed for 116 microorganisms (95.87%), on poly(butylene succinate) (PBS) for 73 microorganisms (60.33%), and on poly(ɛ-caprolactone) (PCL) for 102 microorganisms (84.3%). Moreover, the growth of microorganisms on poly(lactic acid) (PLA) agar plates was observed for 56 microorganisms (46.28%). Based on the 16S rRNA sequence, 10 bacterial strains which showed the highest ability for biodegradation were identified as species belonging to Pseudomonas sp. and Rhodococcus sp. The isolated fungal strains were tested for polycaprolactone films and commercial corn and potato starch bags degradation under laboratory conditions. Strains 16G (based on the analysis of a partial 18S rRNA sequence, identified as Clonostachys rosea) and 16H (identified as Trichoderma sp.) showed the highest capability for biodegradation. A particularly high capability for biodegradation was observed for the strain Clonostachys rosea, which showed 100% degradation of starch films and 52.91% degradation of PCL films in a 30-day shake flask experiment. The main advantage of the microorganisms isolated from Arctic environment is the ability to grow at low temperature and efficient biodegradation under this condition. The data suggest that C. rosea can be used in natural and laboratory conditions for degradations of bioplastics. Text Arctic Spitsbergen PubMed Central (PMC) Arctic AMB Express 7 1 |
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Original Article |
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Original Article Urbanek, Aneta K. Rymowicz, Waldemar Strzelecki, Mateusz C. Kociuba, Waldemar Franczak, Łukasz Mirończuk, Aleksandra M. Isolation and characterization of Arctic microorganisms decomposing bioplastics |
topic_facet |
Original Article |
description |
The increasing amount of plastic waste causes significant environmental pollution. In this study, screening of Arctic microorganisms which are able to degrade bioplastics was performed. In total, 313 microorganisms were isolated from 52 soil samples from the Arctic region (Spitsbergen). Among the isolated microorganisms, 121 (38.66%) showed biodegradation activity. The ability of clear zone formation on emulsified poly(butylene succinate-co-adipate) (PBSA) was observed for 116 microorganisms (95.87%), on poly(butylene succinate) (PBS) for 73 microorganisms (60.33%), and on poly(ɛ-caprolactone) (PCL) for 102 microorganisms (84.3%). Moreover, the growth of microorganisms on poly(lactic acid) (PLA) agar plates was observed for 56 microorganisms (46.28%). Based on the 16S rRNA sequence, 10 bacterial strains which showed the highest ability for biodegradation were identified as species belonging to Pseudomonas sp. and Rhodococcus sp. The isolated fungal strains were tested for polycaprolactone films and commercial corn and potato starch bags degradation under laboratory conditions. Strains 16G (based on the analysis of a partial 18S rRNA sequence, identified as Clonostachys rosea) and 16H (identified as Trichoderma sp.) showed the highest capability for biodegradation. A particularly high capability for biodegradation was observed for the strain Clonostachys rosea, which showed 100% degradation of starch films and 52.91% degradation of PCL films in a 30-day shake flask experiment. The main advantage of the microorganisms isolated from Arctic environment is the ability to grow at low temperature and efficient biodegradation under this condition. The data suggest that C. rosea can be used in natural and laboratory conditions for degradations of bioplastics. |
format |
Text |
author |
Urbanek, Aneta K. Rymowicz, Waldemar Strzelecki, Mateusz C. Kociuba, Waldemar Franczak, Łukasz Mirończuk, Aleksandra M. |
author_facet |
Urbanek, Aneta K. Rymowicz, Waldemar Strzelecki, Mateusz C. Kociuba, Waldemar Franczak, Łukasz Mirończuk, Aleksandra M. |
author_sort |
Urbanek, Aneta K. |
title |
Isolation and characterization of Arctic microorganisms decomposing bioplastics |
title_short |
Isolation and characterization of Arctic microorganisms decomposing bioplastics |
title_full |
Isolation and characterization of Arctic microorganisms decomposing bioplastics |
title_fullStr |
Isolation and characterization of Arctic microorganisms decomposing bioplastics |
title_full_unstemmed |
Isolation and characterization of Arctic microorganisms decomposing bioplastics |
title_sort |
isolation and characterization of arctic microorganisms decomposing bioplastics |
publisher |
Springer Berlin Heidelberg |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503855/ http://www.ncbi.nlm.nih.gov/pubmed/28697585 https://doi.org/10.1186/s13568-017-0448-4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Spitsbergen |
genre_facet |
Arctic Spitsbergen |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503855/ http://www.ncbi.nlm.nih.gov/pubmed/28697585 http://dx.doi.org/10.1186/s13568-017-0448-4 |
op_rights |
© The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s13568-017-0448-4 |
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