Characterization of MHC class I in a long distance migratory wader, the Icelandic black-tailed godwit
The major histocompatibility complex (MHC) encodes proteins that are central for antigen presentation and pathogen elimination. MHC class I (MHC-I) genes have attracted a great deal of interest among researchers in ecology and evolution and have been partly characterized in a wide range of bird spec...
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ftpubmed:oai:pubmedcentral.nih.gov:5486808 2023-05-15T18:49:36+02:00 Characterization of MHC class I in a long distance migratory wader, the Icelandic black-tailed godwit Pardal, Sara Drews, Anna Alves, José A. Ramos, Jaime A. Westerdahl, Helena 2017-05-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486808/ http://www.ncbi.nlm.nih.gov/pubmed/28534224 https://doi.org/10.1007/s00251-017-0993-7 en eng Springer Berlin Heidelberg http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486808/ http://www.ncbi.nlm.nih.gov/pubmed/28534224 http://dx.doi.org/10.1007/s00251-017-0993-7 © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. CC-BY Original Article Text 2017 ftpubmed https://doi.org/10.1007/s00251-017-0993-7 2017-07-16T00:04:19Z The major histocompatibility complex (MHC) encodes proteins that are central for antigen presentation and pathogen elimination. MHC class I (MHC-I) genes have attracted a great deal of interest among researchers in ecology and evolution and have been partly characterized in a wide range of bird species. So far, the main focus has been on species within the bird orders Galliformes and Passeriformes, while Charadriiformes remain vastly underrepresented with only two species studied to date. These two Charadriiformes species exhibit striking differences in MHC-I characteristics and MHC-I diversity. We therefore set out to study a third species within Charadriiformes, the Icelandic subspecies of black-tailed godwits (Limosa limosa islandica). This subspecies is normally confined to parasite-poor environments, and we hence expected low MHC diversity. MHC-I was partially characterized first using Sanger sequencing and then using high-throughput sequencing (MiSeq) in 84 individuals. We verified 47 nucleotide alleles in open reading frame with classical MHC-I characteristics, and each individual godwit had two to seven putatively classical MHC alleles. However, in contrast to previous MHC-I data within Charadriiformes, we did not find any evidence of alleles with low sequence diversity, believed to represent non-classical MHC genes. The diversity and divergence of the godwits MHC-I genes to a large extent fell between the previous estimates within Charadriiformes. However, the MHC genes of the migratory godwits had few sites subject to positive selection, and one possible explanation could be a low exposure to pathogens. Text black-tailed godwit Limosa limosa PubMed Central (PMC) Immunogenetics 69 7 463 478 |
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Original Article |
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Original Article Pardal, Sara Drews, Anna Alves, José A. Ramos, Jaime A. Westerdahl, Helena Characterization of MHC class I in a long distance migratory wader, the Icelandic black-tailed godwit |
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Original Article |
description |
The major histocompatibility complex (MHC) encodes proteins that are central for antigen presentation and pathogen elimination. MHC class I (MHC-I) genes have attracted a great deal of interest among researchers in ecology and evolution and have been partly characterized in a wide range of bird species. So far, the main focus has been on species within the bird orders Galliformes and Passeriformes, while Charadriiformes remain vastly underrepresented with only two species studied to date. These two Charadriiformes species exhibit striking differences in MHC-I characteristics and MHC-I diversity. We therefore set out to study a third species within Charadriiformes, the Icelandic subspecies of black-tailed godwits (Limosa limosa islandica). This subspecies is normally confined to parasite-poor environments, and we hence expected low MHC diversity. MHC-I was partially characterized first using Sanger sequencing and then using high-throughput sequencing (MiSeq) in 84 individuals. We verified 47 nucleotide alleles in open reading frame with classical MHC-I characteristics, and each individual godwit had two to seven putatively classical MHC alleles. However, in contrast to previous MHC-I data within Charadriiformes, we did not find any evidence of alleles with low sequence diversity, believed to represent non-classical MHC genes. The diversity and divergence of the godwits MHC-I genes to a large extent fell between the previous estimates within Charadriiformes. However, the MHC genes of the migratory godwits had few sites subject to positive selection, and one possible explanation could be a low exposure to pathogens. |
format |
Text |
author |
Pardal, Sara Drews, Anna Alves, José A. Ramos, Jaime A. Westerdahl, Helena |
author_facet |
Pardal, Sara Drews, Anna Alves, José A. Ramos, Jaime A. Westerdahl, Helena |
author_sort |
Pardal, Sara |
title |
Characterization of MHC class I in a long distance migratory wader, the Icelandic black-tailed godwit |
title_short |
Characterization of MHC class I in a long distance migratory wader, the Icelandic black-tailed godwit |
title_full |
Characterization of MHC class I in a long distance migratory wader, the Icelandic black-tailed godwit |
title_fullStr |
Characterization of MHC class I in a long distance migratory wader, the Icelandic black-tailed godwit |
title_full_unstemmed |
Characterization of MHC class I in a long distance migratory wader, the Icelandic black-tailed godwit |
title_sort |
characterization of mhc class i in a long distance migratory wader, the icelandic black-tailed godwit |
publisher |
Springer Berlin Heidelberg |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486808/ http://www.ncbi.nlm.nih.gov/pubmed/28534224 https://doi.org/10.1007/s00251-017-0993-7 |
genre |
black-tailed godwit Limosa limosa |
genre_facet |
black-tailed godwit Limosa limosa |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486808/ http://www.ncbi.nlm.nih.gov/pubmed/28534224 http://dx.doi.org/10.1007/s00251-017-0993-7 |
op_rights |
© The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00251-017-0993-7 |
container_title |
Immunogenetics |
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69 |
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7 |
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463 |
op_container_end_page |
478 |
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1766243200596443136 |