Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas
This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V....
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ftpubmed:oai:pubmedcentral.nih.gov:5446674 2023-05-15T15:58:43+02:00 Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas Travers, Marie-Agnès Tourbiez, Delphine Parizadeh, Leïla Haffner, Philippe Kozic-Djellouli, Angélique Aboubaker, Mohamed Koken, Marcel Dégremont, Lionel Lupo, Coralie 2017-05-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446674/ http://www.ncbi.nlm.nih.gov/pubmed/28549482 https://doi.org/10.1186/s13567-017-0438-1 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446674/ http://www.ncbi.nlm.nih.gov/pubmed/28549482 http://dx.doi.org/10.1186/s13567-017-0438-1 © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. CC0 PDM CC-BY Research Article Text 2017 ftpubmed https://doi.org/10.1186/s13567-017-0438-1 2017-06-04T00:28:27Z This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by using seawater containing freshly shed bacteria. These results indicate that, at least with these criteria, despite V. aestuarianus strains genetic diversity, the disease process is similar. The strains isolated after the apparent re-emergence of the bacteria in 2011, do not present a more acute virulence phenotype than the reference strains isolated between 2002 and 2007. Finally, our study provides original and noteworthy data indicating that infected oysters shed bacteria at a level above the threshold of LD50 a few days before they die, meaning that infection is expected to spread in a susceptible population. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Veterinary Research 48 1 |
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Research Article Travers, Marie-Agnès Tourbiez, Delphine Parizadeh, Leïla Haffner, Philippe Kozic-Djellouli, Angélique Aboubaker, Mohamed Koken, Marcel Dégremont, Lionel Lupo, Coralie Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
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Research Article |
description |
This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by using seawater containing freshly shed bacteria. These results indicate that, at least with these criteria, despite V. aestuarianus strains genetic diversity, the disease process is similar. The strains isolated after the apparent re-emergence of the bacteria in 2011, do not present a more acute virulence phenotype than the reference strains isolated between 2002 and 2007. Finally, our study provides original and noteworthy data indicating that infected oysters shed bacteria at a level above the threshold of LD50 a few days before they die, meaning that infection is expected to spread in a susceptible population. |
format |
Text |
author |
Travers, Marie-Agnès Tourbiez, Delphine Parizadeh, Leïla Haffner, Philippe Kozic-Djellouli, Angélique Aboubaker, Mohamed Koken, Marcel Dégremont, Lionel Lupo, Coralie |
author_facet |
Travers, Marie-Agnès Tourbiez, Delphine Parizadeh, Leïla Haffner, Philippe Kozic-Djellouli, Angélique Aboubaker, Mohamed Koken, Marcel Dégremont, Lionel Lupo, Coralie |
author_sort |
Travers, Marie-Agnès |
title |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_short |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_full |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_sort |
several strains, one disease: experimental investigation of vibrio aestuarianus infection parameters in the pacific oyster, crassostrea gigas |
publisher |
BioMed Central |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446674/ http://www.ncbi.nlm.nih.gov/pubmed/28549482 https://doi.org/10.1186/s13567-017-0438-1 |
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Pacific |
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Pacific |
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Crassostrea gigas Pacific oyster |
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Crassostrea gigas Pacific oyster |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446674/ http://www.ncbi.nlm.nih.gov/pubmed/28549482 http://dx.doi.org/10.1186/s13567-017-0438-1 |
op_rights |
© The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
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CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s13567-017-0438-1 |
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Veterinary Research |
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48 |
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1 |
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1766394494363631616 |