Seasonal Change in Bacterial Flora and Biomass in Mountain Snow from the Tateyama Mountains, Japan, Analyzed by 16S rRNA Gene Sequencing and Real-Time PCR
The bacterial flora and biomass in mountain snow from the Tateyama Mountains, Toyama Prefecture, Japan, one of the heaviest snowfall regions in the world, were analyzed by amplified ribosomal DNA restriction analysis followed by 16S rRNA gene sequencing and DNA quantification by real-time PCR. Sampl...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC544271 http://www.ncbi.nlm.nih.gov/pubmed/15640179 https://doi.org/10.1128/AEM.71.1.123-130.2005 |
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ftpubmed:oai:pubmedcentral.nih.gov:544271 2023-05-15T13:52:05+02:00 Seasonal Change in Bacterial Flora and Biomass in Mountain Snow from the Tateyama Mountains, Japan, Analyzed by 16S rRNA Gene Sequencing and Real-Time PCR Segawa, Takahiro Miyamoto, Koji Ushida, Kazunari Agata, Kiyokazu Okada, Norihiro Kohshima, Shiro 2005-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC544271 http://www.ncbi.nlm.nih.gov/pubmed/15640179 https://doi.org/10.1128/AEM.71.1.123-130.2005 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC544271 http://www.ncbi.nlm.nih.gov/pubmed/15640179 http://dx.doi.org/10.1128/AEM.71.1.123-130.2005 Copyright © 2005, American Society for Microbiology Microbial Ecology Text 2005 ftpubmed https://doi.org/10.1128/AEM.71.1.123-130.2005 2013-08-30T04:00:14Z The bacterial flora and biomass in mountain snow from the Tateyama Mountains, Toyama Prefecture, Japan, one of the heaviest snowfall regions in the world, were analyzed by amplified ribosomal DNA restriction analysis followed by 16S rRNA gene sequencing and DNA quantification by real-time PCR. Samples of surface snow collected in various months during the melting season contained a psychrophilic bacterium, Cryobacterium psychrophilum, and two psychrotrophic bacteria, Variovorax paradoxus and Janthinobacterium lividum. Bacterial colonies that developed in an in situ meltwater medium at 4°C were revealed to be V. paradoxus. The biomasses of C. psychrophilum, J. lividum, and V. paradoxus, as estimated by real-time PCR, showed large increases during the melting season from March to October (2.0 × 105-fold, 1.5 × 105-fold, and 1.0 × 104-fold increases, respectively), suggesting their rapid growth in the surface snow. The biomasses of C. psychrophilum and J. lividum increased significantly from March to April, reached a maximum in August, and dropped at the end of the melting season. In contrast, the biomass of V. paradoxus did not increase as rapidly during the early melting season but continued to increase from June until October. The differences in development observed among these bacterial species suggest that their growth was promoted by different nutrients and/or environmental conditions in the snow. Since these three types of bacteria have also been reported to be present in a glacier in Antarctica and a Greenland ice core, they seem to be specialized members of the snow biota that are distributed in snow and ice environments in various parts of the world. Text Antarc* Antarctica glacier Greenland Greenland ice core ice core PubMed Central (PMC) Greenland Applied and Environmental Microbiology 71 1 123 130 |
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Open Polar |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Segawa, Takahiro Miyamoto, Koji Ushida, Kazunari Agata, Kiyokazu Okada, Norihiro Kohshima, Shiro Seasonal Change in Bacterial Flora and Biomass in Mountain Snow from the Tateyama Mountains, Japan, Analyzed by 16S rRNA Gene Sequencing and Real-Time PCR |
topic_facet |
Microbial Ecology |
description |
The bacterial flora and biomass in mountain snow from the Tateyama Mountains, Toyama Prefecture, Japan, one of the heaviest snowfall regions in the world, were analyzed by amplified ribosomal DNA restriction analysis followed by 16S rRNA gene sequencing and DNA quantification by real-time PCR. Samples of surface snow collected in various months during the melting season contained a psychrophilic bacterium, Cryobacterium psychrophilum, and two psychrotrophic bacteria, Variovorax paradoxus and Janthinobacterium lividum. Bacterial colonies that developed in an in situ meltwater medium at 4°C were revealed to be V. paradoxus. The biomasses of C. psychrophilum, J. lividum, and V. paradoxus, as estimated by real-time PCR, showed large increases during the melting season from March to October (2.0 × 105-fold, 1.5 × 105-fold, and 1.0 × 104-fold increases, respectively), suggesting their rapid growth in the surface snow. The biomasses of C. psychrophilum and J. lividum increased significantly from March to April, reached a maximum in August, and dropped at the end of the melting season. In contrast, the biomass of V. paradoxus did not increase as rapidly during the early melting season but continued to increase from June until October. The differences in development observed among these bacterial species suggest that their growth was promoted by different nutrients and/or environmental conditions in the snow. Since these three types of bacteria have also been reported to be present in a glacier in Antarctica and a Greenland ice core, they seem to be specialized members of the snow biota that are distributed in snow and ice environments in various parts of the world. |
format |
Text |
author |
Segawa, Takahiro Miyamoto, Koji Ushida, Kazunari Agata, Kiyokazu Okada, Norihiro Kohshima, Shiro |
author_facet |
Segawa, Takahiro Miyamoto, Koji Ushida, Kazunari Agata, Kiyokazu Okada, Norihiro Kohshima, Shiro |
author_sort |
Segawa, Takahiro |
title |
Seasonal Change in Bacterial Flora and Biomass in Mountain Snow from the Tateyama Mountains, Japan, Analyzed by 16S rRNA Gene Sequencing and Real-Time PCR |
title_short |
Seasonal Change in Bacterial Flora and Biomass in Mountain Snow from the Tateyama Mountains, Japan, Analyzed by 16S rRNA Gene Sequencing and Real-Time PCR |
title_full |
Seasonal Change in Bacterial Flora and Biomass in Mountain Snow from the Tateyama Mountains, Japan, Analyzed by 16S rRNA Gene Sequencing and Real-Time PCR |
title_fullStr |
Seasonal Change in Bacterial Flora and Biomass in Mountain Snow from the Tateyama Mountains, Japan, Analyzed by 16S rRNA Gene Sequencing and Real-Time PCR |
title_full_unstemmed |
Seasonal Change in Bacterial Flora and Biomass in Mountain Snow from the Tateyama Mountains, Japan, Analyzed by 16S rRNA Gene Sequencing and Real-Time PCR |
title_sort |
seasonal change in bacterial flora and biomass in mountain snow from the tateyama mountains, japan, analyzed by 16s rrna gene sequencing and real-time pcr |
publisher |
American Society for Microbiology |
publishDate |
2005 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC544271 http://www.ncbi.nlm.nih.gov/pubmed/15640179 https://doi.org/10.1128/AEM.71.1.123-130.2005 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Antarc* Antarctica glacier Greenland Greenland ice core ice core |
genre_facet |
Antarc* Antarctica glacier Greenland Greenland ice core ice core |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC544271 http://www.ncbi.nlm.nih.gov/pubmed/15640179 http://dx.doi.org/10.1128/AEM.71.1.123-130.2005 |
op_rights |
Copyright © 2005, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.71.1.123-130.2005 |
container_title |
Applied and Environmental Microbiology |
container_volume |
71 |
container_issue |
1 |
container_start_page |
123 |
op_container_end_page |
130 |
_version_ |
1766256319593971712 |