RAD sequencing reveals genomewide divergence between independent invasions of the European green crab (Carcinus maenas) in the Northwest Atlantic
Genomic studies of invasive species can reveal both invasive pathways and functional differences underpinning patterns of colonization success. The European green crab (Carcinus maenas) was initially introduced to eastern North America nearly 200 years ago where it expanded northwards to eastern Nov...
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ftpubmed:oai:pubmedcentral.nih.gov:5395438 2023-05-15T17:45:41+02:00 RAD sequencing reveals genomewide divergence between independent invasions of the European green crab (Carcinus maenas) in the Northwest Atlantic Jeffery, Nicholas W. DiBacco, Claudio Van Wyngaarden, Mallory Hamilton, Lorraine C. Stanley, Ryan R. E. Bernier, Renée FitzGerald, Jennifer Matheson, K. McKenzie, C. H. Nadukkalam Ravindran, Praveen Beiko, Robert Bradbury, Ian R. 2017-03-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395438/ https://doi.org/10.1002/ece3.2872 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395438/ http://dx.doi.org/10.1002/ece3.2872 © 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. CC-BY Original Research Text 2017 ftpubmed https://doi.org/10.1002/ece3.2872 2017-04-23T00:08:14Z Genomic studies of invasive species can reveal both invasive pathways and functional differences underpinning patterns of colonization success. The European green crab (Carcinus maenas) was initially introduced to eastern North America nearly 200 years ago where it expanded northwards to eastern Nova Scotia. A subsequent invasion to Nova Scotia from a northern European source allowed further range expansion, providing a unique opportunity to study the invasion genomics of a species with multiple invasions. Here, we use restriction‐site‐associated DNA sequencing‐derived SNPs to explore fine‐scale genomewide differentiation between these two invasions. We identified 9137 loci from green crab sampled from 11 locations along eastern North America and compared spatial variation to mitochondrial COI sequence variation used previously to characterize these invasions. Overall spatial divergence among invasions was high (pairwise FST ~0.001 to 0.15) and spread across many loci, with a mean FST ~0.052 and 52% of loci examined characterized by FST values >0.05. The majority of the most divergent loci (i.e., outliers, ~1.2%) displayed latitudinal clines in allele frequency highlighting extensive genomic divergence among the invasions. Discriminant analysis of principal components (both neutral and outlier loci) clearly resolved the two invasions spatially and was highly correlated with mitochondrial divergence. Our results reveal extensive cryptic intraspecific genomic diversity associated with differing patterns of colonization success and demonstrates clear utility for genomic approaches to delineating the distribution and colonization success of aquatic invasive species. Text Northwest Atlantic PubMed Central (PMC) Ecology and Evolution 7 8 2513 2524 |
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English |
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Original Research |
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Original Research Jeffery, Nicholas W. DiBacco, Claudio Van Wyngaarden, Mallory Hamilton, Lorraine C. Stanley, Ryan R. E. Bernier, Renée FitzGerald, Jennifer Matheson, K. McKenzie, C. H. Nadukkalam Ravindran, Praveen Beiko, Robert Bradbury, Ian R. RAD sequencing reveals genomewide divergence between independent invasions of the European green crab (Carcinus maenas) in the Northwest Atlantic |
topic_facet |
Original Research |
description |
Genomic studies of invasive species can reveal both invasive pathways and functional differences underpinning patterns of colonization success. The European green crab (Carcinus maenas) was initially introduced to eastern North America nearly 200 years ago where it expanded northwards to eastern Nova Scotia. A subsequent invasion to Nova Scotia from a northern European source allowed further range expansion, providing a unique opportunity to study the invasion genomics of a species with multiple invasions. Here, we use restriction‐site‐associated DNA sequencing‐derived SNPs to explore fine‐scale genomewide differentiation between these two invasions. We identified 9137 loci from green crab sampled from 11 locations along eastern North America and compared spatial variation to mitochondrial COI sequence variation used previously to characterize these invasions. Overall spatial divergence among invasions was high (pairwise FST ~0.001 to 0.15) and spread across many loci, with a mean FST ~0.052 and 52% of loci examined characterized by FST values >0.05. The majority of the most divergent loci (i.e., outliers, ~1.2%) displayed latitudinal clines in allele frequency highlighting extensive genomic divergence among the invasions. Discriminant analysis of principal components (both neutral and outlier loci) clearly resolved the two invasions spatially and was highly correlated with mitochondrial divergence. Our results reveal extensive cryptic intraspecific genomic diversity associated with differing patterns of colonization success and demonstrates clear utility for genomic approaches to delineating the distribution and colonization success of aquatic invasive species. |
format |
Text |
author |
Jeffery, Nicholas W. DiBacco, Claudio Van Wyngaarden, Mallory Hamilton, Lorraine C. Stanley, Ryan R. E. Bernier, Renée FitzGerald, Jennifer Matheson, K. McKenzie, C. H. Nadukkalam Ravindran, Praveen Beiko, Robert Bradbury, Ian R. |
author_facet |
Jeffery, Nicholas W. DiBacco, Claudio Van Wyngaarden, Mallory Hamilton, Lorraine C. Stanley, Ryan R. E. Bernier, Renée FitzGerald, Jennifer Matheson, K. McKenzie, C. H. Nadukkalam Ravindran, Praveen Beiko, Robert Bradbury, Ian R. |
author_sort |
Jeffery, Nicholas W. |
title |
RAD sequencing reveals genomewide divergence between independent invasions of the European green crab (Carcinus maenas) in the Northwest Atlantic |
title_short |
RAD sequencing reveals genomewide divergence between independent invasions of the European green crab (Carcinus maenas) in the Northwest Atlantic |
title_full |
RAD sequencing reveals genomewide divergence between independent invasions of the European green crab (Carcinus maenas) in the Northwest Atlantic |
title_fullStr |
RAD sequencing reveals genomewide divergence between independent invasions of the European green crab (Carcinus maenas) in the Northwest Atlantic |
title_full_unstemmed |
RAD sequencing reveals genomewide divergence between independent invasions of the European green crab (Carcinus maenas) in the Northwest Atlantic |
title_sort |
rad sequencing reveals genomewide divergence between independent invasions of the european green crab (carcinus maenas) in the northwest atlantic |
publisher |
John Wiley and Sons Inc. |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395438/ https://doi.org/10.1002/ece3.2872 |
genre |
Northwest Atlantic |
genre_facet |
Northwest Atlantic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395438/ http://dx.doi.org/10.1002/ece3.2872 |
op_rights |
© 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1002/ece3.2872 |
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Ecology and Evolution |
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7 |
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8 |
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2513 |
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2524 |
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1766148887769251840 |