Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland
Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the che...
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ftpubmed:oai:pubmedcentral.nih.gov:5334632 2023-05-15T16:47:49+02:00 Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland Einarsdottir, Eydis Magnusdottir, Manuela Astarita, Giuseppe Köck, Matthias Ögmundsdottir, Helga M. Thorsteinsdottir, Margret Rapp, Hans Tore Omarsdottir, Sesselja Paglia, Giuseppe 2017-02-22 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334632/ http://www.ncbi.nlm.nih.gov/pubmed/28241423 https://doi.org/10.3390/md15020052 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334632/ http://www.ncbi.nlm.nih.gov/pubmed/28241423 http://dx.doi.org/10.3390/md15020052 © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2017 ftpubmed https://doi.org/10.3390/md15020052 2017-03-19T01:08:58Z Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the chemical composition of the extracts. This analysis highlighted clear differences in the metabolomes of three sponge specimens, and all of them were identified as Haliclona (Rhizoniera) rosea (Bowerbank, 1866). Therefore, these specimens were selected for further investigation. Haliclona rosea metabolomes contained a class of potential key compounds, the 3-alkyl pyridine alkaloids (3-APA) responsible for the cytotoxic activity of the fractions. Several 3-APA compounds were tentatively identified including haliclamines, cyclostellettamines, viscosalines and viscosamines. Among these compounds, cyclostellettamine P was tentatively identified for the first time by using ion mobility MS in time-aligned parallel (TAP) fragmentation mode. In this work, we show the potential of applying metabolomics strategies and in particular the utility of coupling ion mobility with MS for the molecular characterization of sponge specimens. Text Iceland PubMed Central (PMC) Marine Drugs 15 2 52 |
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Article Einarsdottir, Eydis Magnusdottir, Manuela Astarita, Giuseppe Köck, Matthias Ögmundsdottir, Helga M. Thorsteinsdottir, Margret Rapp, Hans Tore Omarsdottir, Sesselja Paglia, Giuseppe Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
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Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the chemical composition of the extracts. This analysis highlighted clear differences in the metabolomes of three sponge specimens, and all of them were identified as Haliclona (Rhizoniera) rosea (Bowerbank, 1866). Therefore, these specimens were selected for further investigation. Haliclona rosea metabolomes contained a class of potential key compounds, the 3-alkyl pyridine alkaloids (3-APA) responsible for the cytotoxic activity of the fractions. Several 3-APA compounds were tentatively identified including haliclamines, cyclostellettamines, viscosalines and viscosamines. Among these compounds, cyclostellettamine P was tentatively identified for the first time by using ion mobility MS in time-aligned parallel (TAP) fragmentation mode. In this work, we show the potential of applying metabolomics strategies and in particular the utility of coupling ion mobility with MS for the molecular characterization of sponge specimens. |
format |
Text |
author |
Einarsdottir, Eydis Magnusdottir, Manuela Astarita, Giuseppe Köck, Matthias Ögmundsdottir, Helga M. Thorsteinsdottir, Margret Rapp, Hans Tore Omarsdottir, Sesselja Paglia, Giuseppe |
author_facet |
Einarsdottir, Eydis Magnusdottir, Manuela Astarita, Giuseppe Köck, Matthias Ögmundsdottir, Helga M. Thorsteinsdottir, Margret Rapp, Hans Tore Omarsdottir, Sesselja Paglia, Giuseppe |
author_sort |
Einarsdottir, Eydis |
title |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_short |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_full |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_fullStr |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_full_unstemmed |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_sort |
metabolic profiling as a screening tool for cytotoxic compounds: identification of 3-alkyl pyridine alkaloids from sponges collected at a shallow water hydrothermal vent site north of iceland |
publisher |
MDPI |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334632/ http://www.ncbi.nlm.nih.gov/pubmed/28241423 https://doi.org/10.3390/md15020052 |
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Iceland |
genre_facet |
Iceland |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334632/ http://www.ncbi.nlm.nih.gov/pubmed/28241423 http://dx.doi.org/10.3390/md15020052 |
op_rights |
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/md15020052 |
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Marine Drugs |
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15 |
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52 |
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1766037926270992384 |