Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation
Direct analysis of membrane lipids by liquid chromatography-electrospray mass spectrometry was used to demonstrate the role of unsaturation in ether lipids in the adaptation of Methanococcoides burtonii to low temperature. A proteomics approach using two-dimensional liquid chromatography-mass spectr...
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ftpubmed:oai:pubmedcentral.nih.gov:532414 2023-05-15T14:02:53+02:00 Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation Nichols, David S. Miller, Matthew R. Davies, Noel W. Goodchild, Amber Raftery, Mark Cavicchioli, Ricardo 2004-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC532414 http://www.ncbi.nlm.nih.gov/pubmed/15576801 https://doi.org/10.1128/JB.186.24.8508-8515.2004 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC532414 http://www.ncbi.nlm.nih.gov/pubmed/15576801 http://dx.doi.org/10.1128/JB.186.24.8508-8515.2004 Copyright © 2004, American Society for Microbiology Physiology and Metabolism Text 2004 ftpubmed https://doi.org/10.1128/JB.186.24.8508-8515.2004 2013-08-30T03:29:45Z Direct analysis of membrane lipids by liquid chromatography-electrospray mass spectrometry was used to demonstrate the role of unsaturation in ether lipids in the adaptation of Methanococcoides burtonii to low temperature. A proteomics approach using two-dimensional liquid chromatography-mass spectrometry was used to identify enzymes involved in lipid biosynthesis, and a pathway for lipid biosynthesis was reconstructed from the M. burtonii draft genome sequence. The major phospholipids were archaeol phosphatidylglycerol, archaeol phosphatidylinositol, hydroxyarchaeol phosphatidylglycerol, and hydroxyarchaeol phosphatidylinositol. All phospholipid classes contained a series of unsaturated analogues, with the degree of unsaturation dependent on phospholipid class. The proportion of unsaturated lipids from cells grown at 4°C was significantly higher than for cells grown at 23°C. 3-Hydroxy-3-methylglutaryl coenzyme A synthase, farnesyl diphosphate synthase, and geranylgeranyl diphosphate synthase were identified in the expressed proteome, and most genes involved in the mevalonate pathway and processes leading to the formation of phosphatidylinositol and phosphatidylglycerol were identified in the genome sequence. In addition, M. burtonii encodes CDP-inositol and CDP-glycerol transferases and a number of homologs of the plant geranylgeranyl reductase. It therefore appears that the unsaturation of lipids may be due to incomplete reduction of an archaeol precursor rather than to a desaturase mechanism. This study shows that cold adaptation in M. burtonii involves specific changes in membrane lipid unsaturation. It also demonstrates that global methods of analysis for lipids and proteomics linked to a draft genome sequence can be effectively combined to infer specific mechanisms of key biological processes. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Journal of Bacteriology 186 24 8508 8515 |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
topic |
Physiology and Metabolism |
spellingShingle |
Physiology and Metabolism Nichols, David S. Miller, Matthew R. Davies, Noel W. Goodchild, Amber Raftery, Mark Cavicchioli, Ricardo Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation |
topic_facet |
Physiology and Metabolism |
description |
Direct analysis of membrane lipids by liquid chromatography-electrospray mass spectrometry was used to demonstrate the role of unsaturation in ether lipids in the adaptation of Methanococcoides burtonii to low temperature. A proteomics approach using two-dimensional liquid chromatography-mass spectrometry was used to identify enzymes involved in lipid biosynthesis, and a pathway for lipid biosynthesis was reconstructed from the M. burtonii draft genome sequence. The major phospholipids were archaeol phosphatidylglycerol, archaeol phosphatidylinositol, hydroxyarchaeol phosphatidylglycerol, and hydroxyarchaeol phosphatidylinositol. All phospholipid classes contained a series of unsaturated analogues, with the degree of unsaturation dependent on phospholipid class. The proportion of unsaturated lipids from cells grown at 4°C was significantly higher than for cells grown at 23°C. 3-Hydroxy-3-methylglutaryl coenzyme A synthase, farnesyl diphosphate synthase, and geranylgeranyl diphosphate synthase were identified in the expressed proteome, and most genes involved in the mevalonate pathway and processes leading to the formation of phosphatidylinositol and phosphatidylglycerol were identified in the genome sequence. In addition, M. burtonii encodes CDP-inositol and CDP-glycerol transferases and a number of homologs of the plant geranylgeranyl reductase. It therefore appears that the unsaturation of lipids may be due to incomplete reduction of an archaeol precursor rather than to a desaturase mechanism. This study shows that cold adaptation in M. burtonii involves specific changes in membrane lipid unsaturation. It also demonstrates that global methods of analysis for lipids and proteomics linked to a draft genome sequence can be effectively combined to infer specific mechanisms of key biological processes. |
format |
Text |
author |
Nichols, David S. Miller, Matthew R. Davies, Noel W. Goodchild, Amber Raftery, Mark Cavicchioli, Ricardo |
author_facet |
Nichols, David S. Miller, Matthew R. Davies, Noel W. Goodchild, Amber Raftery, Mark Cavicchioli, Ricardo |
author_sort |
Nichols, David S. |
title |
Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation |
title_short |
Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation |
title_full |
Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation |
title_fullStr |
Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation |
title_full_unstemmed |
Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation |
title_sort |
cold adaptation in the antarctic archaeon methanococcoides burtonii involves membrane lipid unsaturation |
publisher |
American Society for Microbiology |
publishDate |
2004 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC532414 http://www.ncbi.nlm.nih.gov/pubmed/15576801 https://doi.org/10.1128/JB.186.24.8508-8515.2004 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC532414 http://www.ncbi.nlm.nih.gov/pubmed/15576801 http://dx.doi.org/10.1128/JB.186.24.8508-8515.2004 |
op_rights |
Copyright © 2004, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/JB.186.24.8508-8515.2004 |
container_title |
Journal of Bacteriology |
container_volume |
186 |
container_issue |
24 |
container_start_page |
8508 |
op_container_end_page |
8515 |
_version_ |
1766273328195043328 |