Anti-Biofilm Activity of a Long-Chain Fatty Aldehyde from Antarctic Pseudoalteromonas haloplanktis TAC125 against Staphylococcus epidermidis Biofilm
Staphylococcus epidermidis is a harmless human skin colonizer responsible for ~20% of orthopedic device-related infections due to its capability to form biofilm. Nowadays there is an interest in the development of anti-biofilm molecules. Marine bacteria represent a still underexploited source of bio...
Published in: | Frontiers in Cellular and Infection Microbiology |
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ftpubmed:oai:pubmedcentral.nih.gov:5322152 2023-05-15T13:52:44+02:00 Anti-Biofilm Activity of a Long-Chain Fatty Aldehyde from Antarctic Pseudoalteromonas haloplanktis TAC125 against Staphylococcus epidermidis Biofilm Casillo, Angela Papa, Rosanna Ricciardelli, Annarita Sannino, Filomena Ziaco, Marcello Tilotta, Marco Selan, Laura Marino, Gennaro Corsaro, Maria M. Tutino, Maria L. Artini, Marco Parrilli, Ermenegilda 2017-02-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322152/ https://doi.org/10.3389/fcimb.2017.00046 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322152/ http://dx.doi.org/10.3389/fcimb.2017.00046 Copyright © 2017 Casillo, Papa, Ricciardelli, Sannino, Ziaco, Tilotta, Selan, Marino, Corsaro, Tutino, Artini and Parrilli. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2017 ftpubmed https://doi.org/10.3389/fcimb.2017.00046 2017-03-12T01:05:18Z Staphylococcus epidermidis is a harmless human skin colonizer responsible for ~20% of orthopedic device-related infections due to its capability to form biofilm. Nowadays there is an interest in the development of anti-biofilm molecules. Marine bacteria represent a still underexploited source of biodiversity able to synthesize a broad range of bioactive compounds, including anti-biofilm molecules. Previous results have demonstrated that the culture supernatant of Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125 impairs the formation of S. epidermidis biofilm. Further, evidence supports the hydrophobic nature of the active molecule, which has been suggested to act as a signal molecule. In this paper we describe an efficient activity-guided purification protocol which allowed us to purify this anti-biofilm molecule and structurally characterize it by NMR and mass spectrometry analyses. Our results demonstrate that the anti-biofilm molecule is pentadecanal, a long-chain fatty aldehyde, whose anti-S. epidermidis biofilm activity has been assessed using both static and dynamic biofilm assays. The specificity of its action on S. epidermidis biofilm has been demonstrated by testing chemical analogs of pentadecanal differing either in the length of the aliphatic chain or in their functional group properties. Further, indications of the mode of action of pentadecanal have been collected by studying the bioluminescence of a Vibrio harveyi reporter strain for the detection of autoinducer AI-2 like activities. The data collected suggest that pentadecanal acts as an AI-2 signal. Moreover, the aldehyde metabolic role and synthesis in the Antarctic source strain has been investigated. To the best of our knowledge, this is the first report on the identification of an anti-biofilm molecule form from cold-adapted bacteria and on the action of a long-chain fatty aldehyde acting as an anti-biofilm molecule against S. epidermidis. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Frontiers in Cellular and Infection Microbiology 7 |
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Microbiology |
spellingShingle |
Microbiology Casillo, Angela Papa, Rosanna Ricciardelli, Annarita Sannino, Filomena Ziaco, Marcello Tilotta, Marco Selan, Laura Marino, Gennaro Corsaro, Maria M. Tutino, Maria L. Artini, Marco Parrilli, Ermenegilda Anti-Biofilm Activity of a Long-Chain Fatty Aldehyde from Antarctic Pseudoalteromonas haloplanktis TAC125 against Staphylococcus epidermidis Biofilm |
topic_facet |
Microbiology |
description |
Staphylococcus epidermidis is a harmless human skin colonizer responsible for ~20% of orthopedic device-related infections due to its capability to form biofilm. Nowadays there is an interest in the development of anti-biofilm molecules. Marine bacteria represent a still underexploited source of biodiversity able to synthesize a broad range of bioactive compounds, including anti-biofilm molecules. Previous results have demonstrated that the culture supernatant of Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125 impairs the formation of S. epidermidis biofilm. Further, evidence supports the hydrophobic nature of the active molecule, which has been suggested to act as a signal molecule. In this paper we describe an efficient activity-guided purification protocol which allowed us to purify this anti-biofilm molecule and structurally characterize it by NMR and mass spectrometry analyses. Our results demonstrate that the anti-biofilm molecule is pentadecanal, a long-chain fatty aldehyde, whose anti-S. epidermidis biofilm activity has been assessed using both static and dynamic biofilm assays. The specificity of its action on S. epidermidis biofilm has been demonstrated by testing chemical analogs of pentadecanal differing either in the length of the aliphatic chain or in their functional group properties. Further, indications of the mode of action of pentadecanal have been collected by studying the bioluminescence of a Vibrio harveyi reporter strain for the detection of autoinducer AI-2 like activities. The data collected suggest that pentadecanal acts as an AI-2 signal. Moreover, the aldehyde metabolic role and synthesis in the Antarctic source strain has been investigated. To the best of our knowledge, this is the first report on the identification of an anti-biofilm molecule form from cold-adapted bacteria and on the action of a long-chain fatty aldehyde acting as an anti-biofilm molecule against S. epidermidis. |
format |
Text |
author |
Casillo, Angela Papa, Rosanna Ricciardelli, Annarita Sannino, Filomena Ziaco, Marcello Tilotta, Marco Selan, Laura Marino, Gennaro Corsaro, Maria M. Tutino, Maria L. Artini, Marco Parrilli, Ermenegilda |
author_facet |
Casillo, Angela Papa, Rosanna Ricciardelli, Annarita Sannino, Filomena Ziaco, Marcello Tilotta, Marco Selan, Laura Marino, Gennaro Corsaro, Maria M. Tutino, Maria L. Artini, Marco Parrilli, Ermenegilda |
author_sort |
Casillo, Angela |
title |
Anti-Biofilm Activity of a Long-Chain Fatty Aldehyde from Antarctic Pseudoalteromonas haloplanktis TAC125 against Staphylococcus epidermidis Biofilm |
title_short |
Anti-Biofilm Activity of a Long-Chain Fatty Aldehyde from Antarctic Pseudoalteromonas haloplanktis TAC125 against Staphylococcus epidermidis Biofilm |
title_full |
Anti-Biofilm Activity of a Long-Chain Fatty Aldehyde from Antarctic Pseudoalteromonas haloplanktis TAC125 against Staphylococcus epidermidis Biofilm |
title_fullStr |
Anti-Biofilm Activity of a Long-Chain Fatty Aldehyde from Antarctic Pseudoalteromonas haloplanktis TAC125 against Staphylococcus epidermidis Biofilm |
title_full_unstemmed |
Anti-Biofilm Activity of a Long-Chain Fatty Aldehyde from Antarctic Pseudoalteromonas haloplanktis TAC125 against Staphylococcus epidermidis Biofilm |
title_sort |
anti-biofilm activity of a long-chain fatty aldehyde from antarctic pseudoalteromonas haloplanktis tac125 against staphylococcus epidermidis biofilm |
publisher |
Frontiers Media S.A. |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322152/ https://doi.org/10.3389/fcimb.2017.00046 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322152/ http://dx.doi.org/10.3389/fcimb.2017.00046 |
op_rights |
Copyright © 2017 Casillo, Papa, Ricciardelli, Sannino, Ziaco, Tilotta, Selan, Marino, Corsaro, Tutino, Artini and Parrilli. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fcimb.2017.00046 |
container_title |
Frontiers in Cellular and Infection Microbiology |
container_volume |
7 |
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1766257206269837312 |