Alternative Protein Sources in the Diet Modulate Microbiota and Functionality in the Distal Intestine of Atlantic Salmon (Salmo salar)
The present study aimed to investigate whether alternative dietary protein sources modulate the microbial communities in the distal intestine (DI) of Atlantic salmon, and whether alterations in microbiota profiles are reflected in modifications in host intestinal function and health status. A 48-day...
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ftpubmed:oai:pubmedcentral.nih.gov:5311410 2023-05-15T15:31:44+02:00 Alternative Protein Sources in the Diet Modulate Microbiota and Functionality in the Distal Intestine of Atlantic Salmon (Salmo salar) Gajardo, Karina Jaramillo-Torres, Alexander Kortner, Trond M. Merrifield, Daniel L. Tinsley, John Bakke, Anne Marie Krogdahl, Åshild 2017-02-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5311410/ http://www.ncbi.nlm.nih.gov/pubmed/27986728 https://doi.org/10.1128/AEM.02615-16 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5311410/ http://www.ncbi.nlm.nih.gov/pubmed/27986728 http://dx.doi.org/10.1128/AEM.02615-16 Copyright © 2017 Gajardo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) . CC-BY Food Microbiology Text 2017 ftpubmed https://doi.org/10.1128/AEM.02615-16 2017-02-26T01:05:24Z The present study aimed to investigate whether alternative dietary protein sources modulate the microbial communities in the distal intestine (DI) of Atlantic salmon, and whether alterations in microbiota profiles are reflected in modifications in host intestinal function and health status. A 48-day feeding trial was conducted, in which groups of fish received one of five diets: a reference diet in which fishmeal (diet FM) was the only protein source and four experimental diets with commercially relevant compositions containing alternative ingredients as partial replacements of fishmeal, i.e., poultry meal (diet PM), a mix of soybean meal and wheat gluten (diet SBMWG), a mix of soy protein concentrate and poultry meal (diet SPCPM), and guar meal and wheat gluten (diet GMWG). Samples were taken of DI digesta and mucosa for microbial profiling using high-throughput sequencing and from DI whole tissue for immunohistochemistry and expression profiling of marker genes for gut health. Regardless of diet, there were significant differences between the microbial populations in the digesta and the mucosa in the salmon DI. Microbial richness was higher in the digesta than the mucosa. The digesta-associated bacterial communities were more affected by the diet than the mucosa-associated microbiota. Interestingly, both legume-based diets (SBMWG and GMWG) presented high relative abundance of lactic acid bacteria in addition to alteration in the expression of a salmon gene related to cell proliferation (pcna). It was, however, not possible to ascertain the cause-effect relationship between changes in bacterial communities and the host's intestinal responses to the diets. Text Atlantic salmon Salmo salar PubMed Central (PMC) Applied and Environmental Microbiology 83 5 |
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English |
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Food Microbiology |
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Food Microbiology Gajardo, Karina Jaramillo-Torres, Alexander Kortner, Trond M. Merrifield, Daniel L. Tinsley, John Bakke, Anne Marie Krogdahl, Åshild Alternative Protein Sources in the Diet Modulate Microbiota and Functionality in the Distal Intestine of Atlantic Salmon (Salmo salar) |
topic_facet |
Food Microbiology |
description |
The present study aimed to investigate whether alternative dietary protein sources modulate the microbial communities in the distal intestine (DI) of Atlantic salmon, and whether alterations in microbiota profiles are reflected in modifications in host intestinal function and health status. A 48-day feeding trial was conducted, in which groups of fish received one of five diets: a reference diet in which fishmeal (diet FM) was the only protein source and four experimental diets with commercially relevant compositions containing alternative ingredients as partial replacements of fishmeal, i.e., poultry meal (diet PM), a mix of soybean meal and wheat gluten (diet SBMWG), a mix of soy protein concentrate and poultry meal (diet SPCPM), and guar meal and wheat gluten (diet GMWG). Samples were taken of DI digesta and mucosa for microbial profiling using high-throughput sequencing and from DI whole tissue for immunohistochemistry and expression profiling of marker genes for gut health. Regardless of diet, there were significant differences between the microbial populations in the digesta and the mucosa in the salmon DI. Microbial richness was higher in the digesta than the mucosa. The digesta-associated bacterial communities were more affected by the diet than the mucosa-associated microbiota. Interestingly, both legume-based diets (SBMWG and GMWG) presented high relative abundance of lactic acid bacteria in addition to alteration in the expression of a salmon gene related to cell proliferation (pcna). It was, however, not possible to ascertain the cause-effect relationship between changes in bacterial communities and the host's intestinal responses to the diets. |
format |
Text |
author |
Gajardo, Karina Jaramillo-Torres, Alexander Kortner, Trond M. Merrifield, Daniel L. Tinsley, John Bakke, Anne Marie Krogdahl, Åshild |
author_facet |
Gajardo, Karina Jaramillo-Torres, Alexander Kortner, Trond M. Merrifield, Daniel L. Tinsley, John Bakke, Anne Marie Krogdahl, Åshild |
author_sort |
Gajardo, Karina |
title |
Alternative Protein Sources in the Diet Modulate Microbiota and Functionality in the Distal Intestine of Atlantic Salmon (Salmo salar) |
title_short |
Alternative Protein Sources in the Diet Modulate Microbiota and Functionality in the Distal Intestine of Atlantic Salmon (Salmo salar) |
title_full |
Alternative Protein Sources in the Diet Modulate Microbiota and Functionality in the Distal Intestine of Atlantic Salmon (Salmo salar) |
title_fullStr |
Alternative Protein Sources in the Diet Modulate Microbiota and Functionality in the Distal Intestine of Atlantic Salmon (Salmo salar) |
title_full_unstemmed |
Alternative Protein Sources in the Diet Modulate Microbiota and Functionality in the Distal Intestine of Atlantic Salmon (Salmo salar) |
title_sort |
alternative protein sources in the diet modulate microbiota and functionality in the distal intestine of atlantic salmon (salmo salar) |
publisher |
American Society for Microbiology |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5311410/ http://www.ncbi.nlm.nih.gov/pubmed/27986728 https://doi.org/10.1128/AEM.02615-16 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5311410/ http://www.ncbi.nlm.nih.gov/pubmed/27986728 http://dx.doi.org/10.1128/AEM.02615-16 |
op_rights |
Copyright © 2017 Gajardo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) . |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1128/AEM.02615-16 |
container_title |
Applied and Environmental Microbiology |
container_volume |
83 |
container_issue |
5 |
_version_ |
1766362262309699584 |