Complete genome sequence of Thermus brockianus GE-1 reveals key enzymes of xylan/xylose metabolism
Thermus brockianus strain GE-1 is a thermophilic, Gram-negative, rod-shaped and non-motile bacterium that was isolated from the Geysir geothermal area, Iceland. Like other thermophiles, Thermus species are often used as model organisms to understand the mechanism of action of extremozymes, especiall...
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ftpubmed:oai:pubmedcentral.nih.gov:5292009 2023-05-15T16:51:56+02:00 Complete genome sequence of Thermus brockianus GE-1 reveals key enzymes of xylan/xylose metabolism Schäfers, Christian Blank, Saskia Wiebusch, Sigrid Elleuche, Skander Antranikian, Garabed 2017-02-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292009/ http://www.ncbi.nlm.nih.gov/pubmed/28174620 https://doi.org/10.1186/s40793-017-0225-7 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292009/ http://www.ncbi.nlm.nih.gov/pubmed/28174620 http://dx.doi.org/10.1186/s40793-017-0225-7 © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. CC0 PDM CC-BY Extended Genome Report Text 2017 ftpubmed https://doi.org/10.1186/s40793-017-0225-7 2017-02-12T01:12:49Z Thermus brockianus strain GE-1 is a thermophilic, Gram-negative, rod-shaped and non-motile bacterium that was isolated from the Geysir geothermal area, Iceland. Like other thermophiles, Thermus species are often used as model organisms to understand the mechanism of action of extremozymes, especially focusing on their heat-activity and thermostability. Genome-specific features of T. brockianus GE-1 and their properties further help to explain processes of the adaption of extremophiles at elevated temperatures. Here we analyze the first whole genome sequence of T. brockianus strain GE-1. Insights of the genome sequence and the methodologies that were applied during de novo assembly and annotation are given in detail. The finished genome shows a phred quality value of QV50. The complete genome size is 2.38 Mb, comprising the chromosome (2,035,182 bp), the megaplasmid pTB1 (342,792 bp) and the smaller plasmid pTB2 (10,299 bp). Gene prediction revealed 2,511 genes in total, including 2,458 protein-encoding genes, 53 RNA and 66 pseudo genes. A unique genomic region on megaplasmid pTB1 was identified encoding key enzymes for xylan depolymerization and xylose metabolism. This is in agreement with the growth experiments in which xylan is utilized as sole source of carbon. Accordingly, we identified sequences encoding the xylanase Xyn10, an endoglucanase, the membrane ABC sugar transporter XylH, the xylose-binding protein XylF, the xylose isomerase XylA catalyzing the first step of xylose metabolism and the xylulokinase XylB, responsible for the second step of xylose metabolism. Our data indicate that an ancestor of T. brockianus obtained the ability to use xylose as alternative carbon source by horizontal gene transfer. Text Iceland PubMed Central (PMC) Geysir ENVELOPE(-20.277,-20.277,64.307,64.307) Standards in Genomic Sciences 12 1 |
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Open Polar |
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PubMed Central (PMC) |
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ftpubmed |
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English |
topic |
Extended Genome Report |
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Extended Genome Report Schäfers, Christian Blank, Saskia Wiebusch, Sigrid Elleuche, Skander Antranikian, Garabed Complete genome sequence of Thermus brockianus GE-1 reveals key enzymes of xylan/xylose metabolism |
topic_facet |
Extended Genome Report |
description |
Thermus brockianus strain GE-1 is a thermophilic, Gram-negative, rod-shaped and non-motile bacterium that was isolated from the Geysir geothermal area, Iceland. Like other thermophiles, Thermus species are often used as model organisms to understand the mechanism of action of extremozymes, especially focusing on their heat-activity and thermostability. Genome-specific features of T. brockianus GE-1 and their properties further help to explain processes of the adaption of extremophiles at elevated temperatures. Here we analyze the first whole genome sequence of T. brockianus strain GE-1. Insights of the genome sequence and the methodologies that were applied during de novo assembly and annotation are given in detail. The finished genome shows a phred quality value of QV50. The complete genome size is 2.38 Mb, comprising the chromosome (2,035,182 bp), the megaplasmid pTB1 (342,792 bp) and the smaller plasmid pTB2 (10,299 bp). Gene prediction revealed 2,511 genes in total, including 2,458 protein-encoding genes, 53 RNA and 66 pseudo genes. A unique genomic region on megaplasmid pTB1 was identified encoding key enzymes for xylan depolymerization and xylose metabolism. This is in agreement with the growth experiments in which xylan is utilized as sole source of carbon. Accordingly, we identified sequences encoding the xylanase Xyn10, an endoglucanase, the membrane ABC sugar transporter XylH, the xylose-binding protein XylF, the xylose isomerase XylA catalyzing the first step of xylose metabolism and the xylulokinase XylB, responsible for the second step of xylose metabolism. Our data indicate that an ancestor of T. brockianus obtained the ability to use xylose as alternative carbon source by horizontal gene transfer. |
format |
Text |
author |
Schäfers, Christian Blank, Saskia Wiebusch, Sigrid Elleuche, Skander Antranikian, Garabed |
author_facet |
Schäfers, Christian Blank, Saskia Wiebusch, Sigrid Elleuche, Skander Antranikian, Garabed |
author_sort |
Schäfers, Christian |
title |
Complete genome sequence of Thermus brockianus GE-1 reveals key enzymes of xylan/xylose metabolism |
title_short |
Complete genome sequence of Thermus brockianus GE-1 reveals key enzymes of xylan/xylose metabolism |
title_full |
Complete genome sequence of Thermus brockianus GE-1 reveals key enzymes of xylan/xylose metabolism |
title_fullStr |
Complete genome sequence of Thermus brockianus GE-1 reveals key enzymes of xylan/xylose metabolism |
title_full_unstemmed |
Complete genome sequence of Thermus brockianus GE-1 reveals key enzymes of xylan/xylose metabolism |
title_sort |
complete genome sequence of thermus brockianus ge-1 reveals key enzymes of xylan/xylose metabolism |
publisher |
BioMed Central |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292009/ http://www.ncbi.nlm.nih.gov/pubmed/28174620 https://doi.org/10.1186/s40793-017-0225-7 |
long_lat |
ENVELOPE(-20.277,-20.277,64.307,64.307) |
geographic |
Geysir |
geographic_facet |
Geysir |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292009/ http://www.ncbi.nlm.nih.gov/pubmed/28174620 http://dx.doi.org/10.1186/s40793-017-0225-7 |
op_rights |
© The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
op_rightsnorm |
CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s40793-017-0225-7 |
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Standards in Genomic Sciences |
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12 |
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1 |
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