Examination of Mycobacterium avium subspecies paratuberculosis mixed genotype infections in dairy animals using a whole genome sequencing approach
Many pathogenic mycobacteria are known to cause severe disease in humans and animals. M. avium subspecies paratuberculosis (Map) is the causative agent of Johne’s disease—a chronic wasting disease affecting ruminants such as cattle and sheep, responsible for significant economic losses in the dairy...
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ftpubmed:oai:pubmedcentral.nih.gov:5160890 2023-05-15T17:22:43+02:00 Examination of Mycobacterium avium subspecies paratuberculosis mixed genotype infections in dairy animals using a whole genome sequencing approach Davidson, Fraser W. Ahlstrom, Christina De Buck, Jeroen Whitney, Hugh G. Tahlan, Kapil 2016-12-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5160890/ http://www.ncbi.nlm.nih.gov/pubmed/27994984 https://doi.org/10.7717/peerj.2793 en eng PeerJ Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5160890/ http://www.ncbi.nlm.nih.gov/pubmed/27994984 http://dx.doi.org/10.7717/peerj.2793 ©2016 Davidson et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. CC-BY Genomics Text 2016 ftpubmed https://doi.org/10.7717/peerj.2793 2016-12-25T01:09:19Z Many pathogenic mycobacteria are known to cause severe disease in humans and animals. M. avium subspecies paratuberculosis (Map) is the causative agent of Johne’s disease—a chronic wasting disease affecting ruminants such as cattle and sheep, responsible for significant economic losses in the dairy and beef industries. Due to the lack of treatment options or effective vaccines, mitigating losses can be difficult. In addition, the early stages of Map infection may occur in asymptomatic hosts that continue to shed viable bacteria in their faeces, leading to the infection of other healthy animals. Using multi-locus short sequence repeat (ML-SSR) analysis we previously reported that individual Johne’s positive dairy cattle from farms across the island of Newfoundland were infected by Map with multiple SSR-types simultaneously. The occurrence of multiple mixed genotype infections has the potential to change pathogen and disease dynamics as well as reduce the efficacy of treatments and vaccines. Therefore, we conducted whole genome sequencing (WGS) and single nucleotide polymorphism (SNP) analysis on a subset of these isolates for a more in-depth examination. We also implemented a PCR assay using two discriminatory SNPs and demonstrated the incidence of a mixed infection by three genotypically diverse Map isolates in a single animal. In addition, results show that WGS and SNP analysis can provide a better understanding of the relationship between Map isolates from individual and different animals. In the future such studies on the occurrence of mixed genotype infections could potentially lead to the identification of variable pathogenicity of different genotypes and allow for better tracking of Map isolates for epidemiological studies. Text Newfoundland PubMed Central (PMC) PeerJ 4 e2793 |
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Genomics Davidson, Fraser W. Ahlstrom, Christina De Buck, Jeroen Whitney, Hugh G. Tahlan, Kapil Examination of Mycobacterium avium subspecies paratuberculosis mixed genotype infections in dairy animals using a whole genome sequencing approach |
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Genomics |
description |
Many pathogenic mycobacteria are known to cause severe disease in humans and animals. M. avium subspecies paratuberculosis (Map) is the causative agent of Johne’s disease—a chronic wasting disease affecting ruminants such as cattle and sheep, responsible for significant economic losses in the dairy and beef industries. Due to the lack of treatment options or effective vaccines, mitigating losses can be difficult. In addition, the early stages of Map infection may occur in asymptomatic hosts that continue to shed viable bacteria in their faeces, leading to the infection of other healthy animals. Using multi-locus short sequence repeat (ML-SSR) analysis we previously reported that individual Johne’s positive dairy cattle from farms across the island of Newfoundland were infected by Map with multiple SSR-types simultaneously. The occurrence of multiple mixed genotype infections has the potential to change pathogen and disease dynamics as well as reduce the efficacy of treatments and vaccines. Therefore, we conducted whole genome sequencing (WGS) and single nucleotide polymorphism (SNP) analysis on a subset of these isolates for a more in-depth examination. We also implemented a PCR assay using two discriminatory SNPs and demonstrated the incidence of a mixed infection by three genotypically diverse Map isolates in a single animal. In addition, results show that WGS and SNP analysis can provide a better understanding of the relationship between Map isolates from individual and different animals. In the future such studies on the occurrence of mixed genotype infections could potentially lead to the identification of variable pathogenicity of different genotypes and allow for better tracking of Map isolates for epidemiological studies. |
format |
Text |
author |
Davidson, Fraser W. Ahlstrom, Christina De Buck, Jeroen Whitney, Hugh G. Tahlan, Kapil |
author_facet |
Davidson, Fraser W. Ahlstrom, Christina De Buck, Jeroen Whitney, Hugh G. Tahlan, Kapil |
author_sort |
Davidson, Fraser W. |
title |
Examination of Mycobacterium avium subspecies paratuberculosis mixed genotype infections in dairy animals using a whole genome sequencing approach |
title_short |
Examination of Mycobacterium avium subspecies paratuberculosis mixed genotype infections in dairy animals using a whole genome sequencing approach |
title_full |
Examination of Mycobacterium avium subspecies paratuberculosis mixed genotype infections in dairy animals using a whole genome sequencing approach |
title_fullStr |
Examination of Mycobacterium avium subspecies paratuberculosis mixed genotype infections in dairy animals using a whole genome sequencing approach |
title_full_unstemmed |
Examination of Mycobacterium avium subspecies paratuberculosis mixed genotype infections in dairy animals using a whole genome sequencing approach |
title_sort |
examination of mycobacterium avium subspecies paratuberculosis mixed genotype infections in dairy animals using a whole genome sequencing approach |
publisher |
PeerJ Inc. |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5160890/ http://www.ncbi.nlm.nih.gov/pubmed/27994984 https://doi.org/10.7717/peerj.2793 |
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Newfoundland |
genre_facet |
Newfoundland |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5160890/ http://www.ncbi.nlm.nih.gov/pubmed/27994984 http://dx.doi.org/10.7717/peerj.2793 |
op_rights |
©2016 Davidson et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
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CC-BY |
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https://doi.org/10.7717/peerj.2793 |
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