Psychrophilic Lipase from Arctic Bacterium

A lipase producer psychrophilic microorganism isolated from Arctic sample was studied. The genomic DNA of the isolate was extracted using modified CTAB method. Identification of the isolate by morphological and 16S rRNA sequence analysis revealed that the isolate is closely related to Arthrobacter g...

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Bibliographic Details
Published in:Tropical Life Sciences Research
Main Authors: Ramle, Zakiah, Rahim, Rashidah Abdul
Format: Text
Language:English
Published: Penerbit Universiti Sains Malaysia 2016
Subjects:
Biotechnology and Food Technology
Arctic
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131664/
http://www.ncbi.nlm.nih.gov/pubmed/27965754
https://doi.org/10.21315/tlsr2016.27.3.21
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Summary:A lipase producer psychrophilic microorganism isolated from Arctic sample was studied. The genomic DNA of the isolate was extracted using modified CTAB method. Identification of the isolate by morphological and 16S rRNA sequence analysis revealed that the isolate is closely related to Arthrobacter gangotriensis (97% similarity). A. gangotriensis was determined as positive lipase producer based on the plate screening using specific and sensitive plate assay of Rhodamine B. The PCR result using Arthrobacter sp.’s full lipase gene sequence as the template primers emphasised a possible lipase gene at 900 bp band size. The gene is further cloned in a suitable vector system for expression of lipase.

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