Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi
Biofilms enhance rates of gene exchange, access to specific nutrients, and cell survivability. Haloarchaea in Deep Lake, Antarctica, are characterized by high rates of intergenera gene exchange, metabolic specialization that promotes niche adaptation, and are exposed to high levels of UV-irradiation...
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ftpubmed:oai:pubmedcentral.nih.gov:5118699 2023-05-15T13:31:34+02:00 Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi Liao, Y. Williams, T. J. Ye, J. Charlesworth, J. Burns, B. P. Poljak, A. Raftery, M. J. Cavicchioli, R. 2016-11-22 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118699/ http://www.ncbi.nlm.nih.gov/pubmed/27874045 https://doi.org/10.1038/srep37454 en eng Nature Publishing Group http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118699/ http://www.ncbi.nlm.nih.gov/pubmed/27874045 http://dx.doi.org/10.1038/srep37454 Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ CC-BY Article Text 2016 ftpubmed https://doi.org/10.1038/srep37454 2016-12-04T01:27:40Z Biofilms enhance rates of gene exchange, access to specific nutrients, and cell survivability. Haloarchaea in Deep Lake, Antarctica, are characterized by high rates of intergenera gene exchange, metabolic specialization that promotes niche adaptation, and are exposed to high levels of UV-irradiation in summer. Halorubrum lacusprofundi from Deep Lake has previously been reported to form biofilms. Here we defined growth conditions that promoted the formation of biofilms and used microscopy and enzymatic digestion of extracellular material to characterize biofilm structures. Extracellular DNA was found to be critical to biofilms, with cell surface proteins and quorum sensing also implicated in biofilm formation. Quantitative proteomics was used to define pathways and cellular processes involved in forming biofilms; these included enhanced purine synthesis and specific cell surface proteins involved in DNA metabolism; post-translational modification of cell surface proteins; specific pathways of carbon metabolism involving acetyl-CoA; and specific responses to oxidative stress. The study provides a new level of understanding about the molecular mechanisms involved in biofilm formation of this important member of the Deep Lake community. Text Antarc* Antarctic Antarctica PubMed Central (PMC) Antarctic The Antarctic Scientific Reports 6 1 |
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Article Liao, Y. Williams, T. J. Ye, J. Charlesworth, J. Burns, B. P. Poljak, A. Raftery, M. J. Cavicchioli, R. Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi |
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Biofilms enhance rates of gene exchange, access to specific nutrients, and cell survivability. Haloarchaea in Deep Lake, Antarctica, are characterized by high rates of intergenera gene exchange, metabolic specialization that promotes niche adaptation, and are exposed to high levels of UV-irradiation in summer. Halorubrum lacusprofundi from Deep Lake has previously been reported to form biofilms. Here we defined growth conditions that promoted the formation of biofilms and used microscopy and enzymatic digestion of extracellular material to characterize biofilm structures. Extracellular DNA was found to be critical to biofilms, with cell surface proteins and quorum sensing also implicated in biofilm formation. Quantitative proteomics was used to define pathways and cellular processes involved in forming biofilms; these included enhanced purine synthesis and specific cell surface proteins involved in DNA metabolism; post-translational modification of cell surface proteins; specific pathways of carbon metabolism involving acetyl-CoA; and specific responses to oxidative stress. The study provides a new level of understanding about the molecular mechanisms involved in biofilm formation of this important member of the Deep Lake community. |
format |
Text |
author |
Liao, Y. Williams, T. J. Ye, J. Charlesworth, J. Burns, B. P. Poljak, A. Raftery, M. J. Cavicchioli, R. |
author_facet |
Liao, Y. Williams, T. J. Ye, J. Charlesworth, J. Burns, B. P. Poljak, A. Raftery, M. J. Cavicchioli, R. |
author_sort |
Liao, Y. |
title |
Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi |
title_short |
Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi |
title_full |
Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi |
title_fullStr |
Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi |
title_full_unstemmed |
Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi |
title_sort |
morphological and proteomic analysis of biofilms from the antarctic archaeon, halorubrum lacusprofundi |
publisher |
Nature Publishing Group |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118699/ http://www.ncbi.nlm.nih.gov/pubmed/27874045 https://doi.org/10.1038/srep37454 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
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Antarc* Antarctic Antarctica |
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Antarc* Antarctic Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118699/ http://www.ncbi.nlm.nih.gov/pubmed/27874045 http://dx.doi.org/10.1038/srep37454 |
op_rights |
Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
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CC-BY |
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https://doi.org/10.1038/srep37454 |
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Scientific Reports |
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6 |
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1 |
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1766018988306857984 |