Genomic conservation of erythropoietic microRNAs (erythromiRs) in white-blooded Antarctic icefish
White-blooded Antarctic crocodile icefish are the only vertebrates known to lack functional hemoglobin genes and red blood cells throughout their lives. We do not yet know, however, whether extinction of hemoglobin genes preceded loss of red blood cells or vice versa, nor whether erythropoiesis regu...
Published in: | Marine Genomics |
---|---|
Main Authors: | , , |
Format: | Text |
Language: | English |
Published: |
2016
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108692/ http://www.ncbi.nlm.nih.gov/pubmed/27189439 https://doi.org/10.1016/j.margen.2016.04.013 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:5108692 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:5108692 2023-05-15T13:40:24+02:00 Genomic conservation of erythropoietic microRNAs (erythromiRs) in white-blooded Antarctic icefish Desvignes, Thomas Detrich, H. William Postlethwait, John H. 2016-05-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108692/ http://www.ncbi.nlm.nih.gov/pubmed/27189439 https://doi.org/10.1016/j.margen.2016.04.013 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108692/ http://www.ncbi.nlm.nih.gov/pubmed/27189439 http://dx.doi.org/10.1016/j.margen.2016.04.013 Article Text 2016 ftpubmed https://doi.org/10.1016/j.margen.2016.04.013 2017-12-03T01:06:21Z White-blooded Antarctic crocodile icefish are the only vertebrates known to lack functional hemoglobin genes and red blood cells throughout their lives. We do not yet know, however, whether extinction of hemoglobin genes preceded loss of red blood cells or vice versa, nor whether erythropoiesis regulators disappeared along with hemoglobin genes in this erythrocyte-null clade. Several microRNAs, which we here call erythromiRs, are expressed primarily in developing red blood cells in zebrafish, mouse, and humans. Abrogating some erythromiRs, like Mir144 and Mir451a, leads to profound anemia, demonstrating a functional role in erythropoiesis. Here, we tested two not mutually exclusive hypotheses: 1) that the loss of one or more erythromiR genes extinguished the erythropoietic program of icefish and/or led to the loss of globin gene expression through pseudogenization; and 2) that some erythromiR genes were secondarily lost after the loss of functional hemoglobin and red blood cells in icefish. We explored smallRNA transcriptomes generated from the hematopoietic kidney marrow of four Antarctic notothenioids: two red-blooded species (bullhead notothen Notothenia coriiceps and emerald notothen Trematomus bernacchii) and two white-blooded icefish (blackfin icefish Chaenocephalus aceratus and hooknose icefish Chionodraco hamatus). The N. coriiceps genome assembly anchored analyses. Results showed that, like the two red-blooded species, the blackfin icefish genome possessed and the marrow expressed all known erythromiRs. This result indicates that loss of hemoglobin and red blood cells in icefish was not caused by loss of known erythromiR genes. Furthermore, expression of only one erythromiR, mir96, appears to have been lost after the loss of red blood cells and hemoglobin – expression was not detected in the erythropoietic organ of hooknose icefish but was present in blackfin icefish. All other erythromiRs investigated, including mir144 and mir451a, were expressed by all four species and thus are present in the genomes ... Text Antarc* Antarctic Icefish PubMed Central (PMC) Antarctic Marine Genomics 30 27 34 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Desvignes, Thomas Detrich, H. William Postlethwait, John H. Genomic conservation of erythropoietic microRNAs (erythromiRs) in white-blooded Antarctic icefish |
topic_facet |
Article |
description |
White-blooded Antarctic crocodile icefish are the only vertebrates known to lack functional hemoglobin genes and red blood cells throughout their lives. We do not yet know, however, whether extinction of hemoglobin genes preceded loss of red blood cells or vice versa, nor whether erythropoiesis regulators disappeared along with hemoglobin genes in this erythrocyte-null clade. Several microRNAs, which we here call erythromiRs, are expressed primarily in developing red blood cells in zebrafish, mouse, and humans. Abrogating some erythromiRs, like Mir144 and Mir451a, leads to profound anemia, demonstrating a functional role in erythropoiesis. Here, we tested two not mutually exclusive hypotheses: 1) that the loss of one or more erythromiR genes extinguished the erythropoietic program of icefish and/or led to the loss of globin gene expression through pseudogenization; and 2) that some erythromiR genes were secondarily lost after the loss of functional hemoglobin and red blood cells in icefish. We explored smallRNA transcriptomes generated from the hematopoietic kidney marrow of four Antarctic notothenioids: two red-blooded species (bullhead notothen Notothenia coriiceps and emerald notothen Trematomus bernacchii) and two white-blooded icefish (blackfin icefish Chaenocephalus aceratus and hooknose icefish Chionodraco hamatus). The N. coriiceps genome assembly anchored analyses. Results showed that, like the two red-blooded species, the blackfin icefish genome possessed and the marrow expressed all known erythromiRs. This result indicates that loss of hemoglobin and red blood cells in icefish was not caused by loss of known erythromiR genes. Furthermore, expression of only one erythromiR, mir96, appears to have been lost after the loss of red blood cells and hemoglobin – expression was not detected in the erythropoietic organ of hooknose icefish but was present in blackfin icefish. All other erythromiRs investigated, including mir144 and mir451a, were expressed by all four species and thus are present in the genomes ... |
format |
Text |
author |
Desvignes, Thomas Detrich, H. William Postlethwait, John H. |
author_facet |
Desvignes, Thomas Detrich, H. William Postlethwait, John H. |
author_sort |
Desvignes, Thomas |
title |
Genomic conservation of erythropoietic microRNAs (erythromiRs) in white-blooded Antarctic icefish |
title_short |
Genomic conservation of erythropoietic microRNAs (erythromiRs) in white-blooded Antarctic icefish |
title_full |
Genomic conservation of erythropoietic microRNAs (erythromiRs) in white-blooded Antarctic icefish |
title_fullStr |
Genomic conservation of erythropoietic microRNAs (erythromiRs) in white-blooded Antarctic icefish |
title_full_unstemmed |
Genomic conservation of erythropoietic microRNAs (erythromiRs) in white-blooded Antarctic icefish |
title_sort |
genomic conservation of erythropoietic micrornas (erythromirs) in white-blooded antarctic icefish |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108692/ http://www.ncbi.nlm.nih.gov/pubmed/27189439 https://doi.org/10.1016/j.margen.2016.04.013 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Icefish |
genre_facet |
Antarc* Antarctic Icefish |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108692/ http://www.ncbi.nlm.nih.gov/pubmed/27189439 http://dx.doi.org/10.1016/j.margen.2016.04.013 |
op_doi |
https://doi.org/10.1016/j.margen.2016.04.013 |
container_title |
Marine Genomics |
container_volume |
30 |
container_start_page |
27 |
op_container_end_page |
34 |
_version_ |
1766133200344580096 |