Experimental piscine alphavirus RNA recombination in vivo yields both viable virus and defective viral RNA
RNA recombination in non-segmented RNA viruses is important for viral evolution and documented for several virus species through in vitro studies. Here we confirm viral RNA recombination in vivo using an alphavirus, the SAV3 subtype of Salmon pancreas disease virus. The virus causes pancreas disease...
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ftpubmed:oai:pubmedcentral.nih.gov:5090867 2023-05-15T15:31:09+02:00 Experimental piscine alphavirus RNA recombination in vivo yields both viable virus and defective viral RNA Petterson, Elin Guo, Tz-Chun Evensen, Øystein Mikalsen, Aase B. 2016-11-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090867/ http://www.ncbi.nlm.nih.gov/pubmed/27805034 https://doi.org/10.1038/srep36317 en eng Nature Publishing Group http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090867/ http://www.ncbi.nlm.nih.gov/pubmed/27805034 http://dx.doi.org/10.1038/srep36317 Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ CC-BY Article Text 2016 ftpubmed https://doi.org/10.1038/srep36317 2016-11-13T01:10:21Z RNA recombination in non-segmented RNA viruses is important for viral evolution and documented for several virus species through in vitro studies. Here we confirm viral RNA recombination in vivo using an alphavirus, the SAV3 subtype of Salmon pancreas disease virus. The virus causes pancreas disease in Atlantic salmon and heavy losses in European salmonid aquaculture. Atlantic salmon were injected with a SAV3 6K-gene deleted cDNA plasmid, encoding a non-viable variant of SAV3, together with a helper cDNA plasmid encoding structural proteins and 6K only. Later, SAV3-specific RNA was detected and recombination of viral RNA was confirmed. Virus was grown from plasmid-injected fish and shown to infect and cause pathology in salmon. Subsequent cloning of PCR products confirming recombination, documented imprecise homologous recombination creating RNA deletion variants in fish injected with cDNA plasmid, corresponding with deletion variants previously found in SAV3 from the field. This is the first experimental documentation of alphavirus RNA recombination in an animal model and provides new insight into the production of defective virus RNA. Text Atlantic salmon PubMed Central (PMC) Scientific Reports 6 1 |
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Article Petterson, Elin Guo, Tz-Chun Evensen, Øystein Mikalsen, Aase B. Experimental piscine alphavirus RNA recombination in vivo yields both viable virus and defective viral RNA |
topic_facet |
Article |
description |
RNA recombination in non-segmented RNA viruses is important for viral evolution and documented for several virus species through in vitro studies. Here we confirm viral RNA recombination in vivo using an alphavirus, the SAV3 subtype of Salmon pancreas disease virus. The virus causes pancreas disease in Atlantic salmon and heavy losses in European salmonid aquaculture. Atlantic salmon were injected with a SAV3 6K-gene deleted cDNA plasmid, encoding a non-viable variant of SAV3, together with a helper cDNA plasmid encoding structural proteins and 6K only. Later, SAV3-specific RNA was detected and recombination of viral RNA was confirmed. Virus was grown from plasmid-injected fish and shown to infect and cause pathology in salmon. Subsequent cloning of PCR products confirming recombination, documented imprecise homologous recombination creating RNA deletion variants in fish injected with cDNA plasmid, corresponding with deletion variants previously found in SAV3 from the field. This is the first experimental documentation of alphavirus RNA recombination in an animal model and provides new insight into the production of defective virus RNA. |
format |
Text |
author |
Petterson, Elin Guo, Tz-Chun Evensen, Øystein Mikalsen, Aase B. |
author_facet |
Petterson, Elin Guo, Tz-Chun Evensen, Øystein Mikalsen, Aase B. |
author_sort |
Petterson, Elin |
title |
Experimental piscine alphavirus RNA recombination in vivo yields both viable virus and defective viral RNA |
title_short |
Experimental piscine alphavirus RNA recombination in vivo yields both viable virus and defective viral RNA |
title_full |
Experimental piscine alphavirus RNA recombination in vivo yields both viable virus and defective viral RNA |
title_fullStr |
Experimental piscine alphavirus RNA recombination in vivo yields both viable virus and defective viral RNA |
title_full_unstemmed |
Experimental piscine alphavirus RNA recombination in vivo yields both viable virus and defective viral RNA |
title_sort |
experimental piscine alphavirus rna recombination in vivo yields both viable virus and defective viral rna |
publisher |
Nature Publishing Group |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090867/ http://www.ncbi.nlm.nih.gov/pubmed/27805034 https://doi.org/10.1038/srep36317 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090867/ http://www.ncbi.nlm.nih.gov/pubmed/27805034 http://dx.doi.org/10.1038/srep36317 |
op_rights |
Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
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CC-BY |
op_doi |
https://doi.org/10.1038/srep36317 |
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Scientific Reports |
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6 |
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1 |
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1766361638086115328 |