A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L.
Liverwort Blasia pusilla L. recruits soil nitrogen-fixing cyanobacteria of genus Nostoc as symbiotic partners. In this work we compared Nostoc community composition inside the plants and in the soil around them from two distant locations in Northern Norway. STRR fingerprinting and 16S rDNA phylogeny...
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ftpubmed:oai:pubmedcentral.nih.gov:5088731 2023-05-15T17:43:31+02:00 A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. Liaimer, Anton Jensen, John B. Dittmann, Elke 2016-11-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088731/ http://www.ncbi.nlm.nih.gov/pubmed/27847500 https://doi.org/10.3389/fmicb.2016.01693 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088731/ http://www.ncbi.nlm.nih.gov/pubmed/27847500 http://dx.doi.org/10.3389/fmicb.2016.01693 Copyright © 2016 Liaimer, Jensen and Dittmann. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2016 ftpubmed https://doi.org/10.3389/fmicb.2016.01693 2016-11-20T01:06:49Z Liverwort Blasia pusilla L. recruits soil nitrogen-fixing cyanobacteria of genus Nostoc as symbiotic partners. In this work we compared Nostoc community composition inside the plants and in the soil around them from two distant locations in Northern Norway. STRR fingerprinting and 16S rDNA phylogeny reconstruction showed a remarkable local diversity among isolates assigned to several Nostoc clades. An extensive web of negative allelopathic interactions was recorded at an agricultural site, but not at the undisturbed natural site. The cell extracts of the cyanobacteria did not show antimicrobial activities, but four isolates were shown to be cytotoxic to human cells. The secondary metabolite profiles of the isolates were mapped by MALDI-TOF MS, and the most prominent ions were further analyzed by Q-TOF for MS/MS aided identification. Symbiotic isolates produced a great variety of small peptide-like substances, most of which lack any record in the databases. Among identified compounds we found microcystin and nodularin variants toxic to eukaryotic cells. Microcystin producing chemotypes were dominating as symbiotic recruits but not in the free-living community. In addition, we were able to identify several novel aeruginosins and banyaside-like compounds, as well as nostocyclopeptides and nosperin. Text Northern Norway PubMed Central (PMC) Norway Frontiers in Microbiology 7 |
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ftpubmed |
language |
English |
topic |
Microbiology |
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Microbiology Liaimer, Anton Jensen, John B. Dittmann, Elke A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. |
topic_facet |
Microbiology |
description |
Liverwort Blasia pusilla L. recruits soil nitrogen-fixing cyanobacteria of genus Nostoc as symbiotic partners. In this work we compared Nostoc community composition inside the plants and in the soil around them from two distant locations in Northern Norway. STRR fingerprinting and 16S rDNA phylogeny reconstruction showed a remarkable local diversity among isolates assigned to several Nostoc clades. An extensive web of negative allelopathic interactions was recorded at an agricultural site, but not at the undisturbed natural site. The cell extracts of the cyanobacteria did not show antimicrobial activities, but four isolates were shown to be cytotoxic to human cells. The secondary metabolite profiles of the isolates were mapped by MALDI-TOF MS, and the most prominent ions were further analyzed by Q-TOF for MS/MS aided identification. Symbiotic isolates produced a great variety of small peptide-like substances, most of which lack any record in the databases. Among identified compounds we found microcystin and nodularin variants toxic to eukaryotic cells. Microcystin producing chemotypes were dominating as symbiotic recruits but not in the free-living community. In addition, we were able to identify several novel aeruginosins and banyaside-like compounds, as well as nostocyclopeptides and nosperin. |
format |
Text |
author |
Liaimer, Anton Jensen, John B. Dittmann, Elke |
author_facet |
Liaimer, Anton Jensen, John B. Dittmann, Elke |
author_sort |
Liaimer, Anton |
title |
A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. |
title_short |
A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. |
title_full |
A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. |
title_fullStr |
A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. |
title_full_unstemmed |
A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. |
title_sort |
genetic and chemical perspective on symbiotic recruitment of cyanobacteria of the genus nostoc into the host plant blasia pusilla l. |
publisher |
Frontiers Media S.A. |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088731/ http://www.ncbi.nlm.nih.gov/pubmed/27847500 https://doi.org/10.3389/fmicb.2016.01693 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088731/ http://www.ncbi.nlm.nih.gov/pubmed/27847500 http://dx.doi.org/10.3389/fmicb.2016.01693 |
op_rights |
Copyright © 2016 Liaimer, Jensen and Dittmann. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2016.01693 |
container_title |
Frontiers in Microbiology |
container_volume |
7 |
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1766145609084960768 |