Summer Abundance and Distribution of Proteorhodopsin Genes in the Western Arctic Ocean
Proteorhodopsins (PR) are phylogenetically diverse and highly expressed proton pumps in marine bacterial communities. The phylogenetic diversity and in situ expression of the main PR groups in polar off-shore, coastal and estuarine waters is poorly known and their abundance has not yet been reported...
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ftpubmed:oai:pubmedcentral.nih.gov:5061748 2023-05-15T14:51:40+02:00 Summer Abundance and Distribution of Proteorhodopsin Genes in the Western Arctic Ocean Boeuf, Dominique Lami, Raphaël Cunnington, Emelyne Jeanthon, Christian 2016-10-13 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061748/ https://doi.org/10.3389/fmicb.2016.01584 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061748/ http://dx.doi.org/10.3389/fmicb.2016.01584 Copyright © 2016 Boeuf, Lami, Cunnington and Jeanthon. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2016 ftpubmed https://doi.org/10.3389/fmicb.2016.01584 2016-10-30T00:08:23Z Proteorhodopsins (PR) are phylogenetically diverse and highly expressed proton pumps in marine bacterial communities. The phylogenetic diversity and in situ expression of the main PR groups in polar off-shore, coastal and estuarine waters is poorly known and their abundance has not yet been reported. Here, we show that PR gene sequences of the southern Beaufort Sea including MacKenzie shelf and estuary are mainly affiliated to Gammaproteobacteria, Alphaproteobacteria, and Bacteroidetes. Substantial overlap (78%) between DNA- and cDNA-based librairies indicated in situ PR transcription within a large fraction of PR-containing community. Sets of specific qPCR primers were designed to measure the absolute abundances of the major PR types. Spatial and depth profiles showed that PR-containing bacteria were abundant throughout the photic zone, comprising up to 45% of total bacteria. Although their abundance varied greatly with location and depth, Alphaproteobacteria predominated in the PR community in all water masses, with SAR11 as the major PR type. Low nutrient concentrations rather than light were the environmental drivers that best explained the abundance and distribution of arctic PR types. Together, our data suggests that PR-based phototrophy could be the major phototrophic prokaryotic process during the Arctic Ocean summer. Text Arctic Arctic Ocean Beaufort Sea Mackenzie Shelf PubMed Central (PMC) Arctic Arctic Ocean Frontiers in Microbiology 7 |
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PubMed Central (PMC) |
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English |
topic |
Microbiology |
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Microbiology Boeuf, Dominique Lami, Raphaël Cunnington, Emelyne Jeanthon, Christian Summer Abundance and Distribution of Proteorhodopsin Genes in the Western Arctic Ocean |
topic_facet |
Microbiology |
description |
Proteorhodopsins (PR) are phylogenetically diverse and highly expressed proton pumps in marine bacterial communities. The phylogenetic diversity and in situ expression of the main PR groups in polar off-shore, coastal and estuarine waters is poorly known and their abundance has not yet been reported. Here, we show that PR gene sequences of the southern Beaufort Sea including MacKenzie shelf and estuary are mainly affiliated to Gammaproteobacteria, Alphaproteobacteria, and Bacteroidetes. Substantial overlap (78%) between DNA- and cDNA-based librairies indicated in situ PR transcription within a large fraction of PR-containing community. Sets of specific qPCR primers were designed to measure the absolute abundances of the major PR types. Spatial and depth profiles showed that PR-containing bacteria were abundant throughout the photic zone, comprising up to 45% of total bacteria. Although their abundance varied greatly with location and depth, Alphaproteobacteria predominated in the PR community in all water masses, with SAR11 as the major PR type. Low nutrient concentrations rather than light were the environmental drivers that best explained the abundance and distribution of arctic PR types. Together, our data suggests that PR-based phototrophy could be the major phototrophic prokaryotic process during the Arctic Ocean summer. |
format |
Text |
author |
Boeuf, Dominique Lami, Raphaël Cunnington, Emelyne Jeanthon, Christian |
author_facet |
Boeuf, Dominique Lami, Raphaël Cunnington, Emelyne Jeanthon, Christian |
author_sort |
Boeuf, Dominique |
title |
Summer Abundance and Distribution of Proteorhodopsin Genes in the Western Arctic Ocean |
title_short |
Summer Abundance and Distribution of Proteorhodopsin Genes in the Western Arctic Ocean |
title_full |
Summer Abundance and Distribution of Proteorhodopsin Genes in the Western Arctic Ocean |
title_fullStr |
Summer Abundance and Distribution of Proteorhodopsin Genes in the Western Arctic Ocean |
title_full_unstemmed |
Summer Abundance and Distribution of Proteorhodopsin Genes in the Western Arctic Ocean |
title_sort |
summer abundance and distribution of proteorhodopsin genes in the western arctic ocean |
publisher |
Frontiers Media S.A. |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061748/ https://doi.org/10.3389/fmicb.2016.01584 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean Beaufort Sea Mackenzie Shelf |
genre_facet |
Arctic Arctic Ocean Beaufort Sea Mackenzie Shelf |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061748/ http://dx.doi.org/10.3389/fmicb.2016.01584 |
op_rights |
Copyright © 2016 Boeuf, Lami, Cunnington and Jeanthon. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2016.01584 |
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Frontiers in Microbiology |
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7 |
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1766322797480509440 |