Characterization and expression of sweetfish (Plecoglossus altivelis) cathepsin D
Cathepsin D (CTSD) is a lysosomal acidic endoproteinase that plays an important role in immune response. In this study, we obtained sweetfish (Plecoglossus altivelis) CTSD (PaCTSD) via de-novo transcriptome sequencing of sweetfish macrophages. The full length cDNA sequence of PaCTSD was 1955 bp enco...
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ftpubmed:oai:pubmedcentral.nih.gov:5031676 2023-05-15T15:32:30+02:00 Characterization and expression of sweetfish (Plecoglossus altivelis) cathepsin D Yu, JIAO Chang-Hong, LI Xin-Jiang, LU Jiong, CHEN 2014-07-18 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031676/ http://www.ncbi.nlm.nih.gov/pubmed/25017748 https://doi.org/10.13918/j.issn.2095-8137.2014.4.294 en eng Science Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031676/ http://www.ncbi.nlm.nih.gov/pubmed/25017748 http://dx.doi.org/10.13918/j.issn.2095-8137.2014.4.294 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Articles Text 2014 ftpubmed https://doi.org/10.13918/j.issn.2095-8137.2014.4.294 2016-10-02T00:13:16Z Cathepsin D (CTSD) is a lysosomal acidic endoproteinase that plays an important role in immune response. In this study, we obtained sweetfish (Plecoglossus altivelis) CTSD (PaCTSD) via de-novo transcriptome sequencing of sweetfish macrophages. The full length cDNA sequence of PaCTSD was 1955 bp encoding a propeptide of 397 amino acids. The deduced protein had a calculated molecular weight of 43.17×103. Multiple alignment with other known CTSD amino acid sequences revealed amino acid conservation through the teleosts. Phylogenetic tree analysis showed that PaCTSD grouped tightly with other fish CTSD, and was close to that of Atlantic salmon and rainbow trout. Subsequently, PaCTSD was prokaryotically expressed and refolded by the urea gradient method on a nickel-nitrilotriacetic acid column. Enzyme activity analysis showed that PaCTSD exhibited pH-dependent proteolytic activity. Quantitative real-time PCR showed that PaCTSD mRNA was expressed in all detected tissues in healthy sweetfish. The highest expression was observed in the spleen and white blood cells, followed by liver, head-kidney, kidney, intestine, gill, and muscle. After Listonella anguillarum infection, PaCTSD transcripts were up-regulated significantly in liver, spleen, white blood cells, and head-kidney of sweetfish. In summary, PaCTSD has proteolytic activity and is closely involved in the immune response of sweetfish. Text Atlantic salmon PubMed Central (PMC) |
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Articles Yu, JIAO Chang-Hong, LI Xin-Jiang, LU Jiong, CHEN Characterization and expression of sweetfish (Plecoglossus altivelis) cathepsin D |
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Cathepsin D (CTSD) is a lysosomal acidic endoproteinase that plays an important role in immune response. In this study, we obtained sweetfish (Plecoglossus altivelis) CTSD (PaCTSD) via de-novo transcriptome sequencing of sweetfish macrophages. The full length cDNA sequence of PaCTSD was 1955 bp encoding a propeptide of 397 amino acids. The deduced protein had a calculated molecular weight of 43.17×103. Multiple alignment with other known CTSD amino acid sequences revealed amino acid conservation through the teleosts. Phylogenetic tree analysis showed that PaCTSD grouped tightly with other fish CTSD, and was close to that of Atlantic salmon and rainbow trout. Subsequently, PaCTSD was prokaryotically expressed and refolded by the urea gradient method on a nickel-nitrilotriacetic acid column. Enzyme activity analysis showed that PaCTSD exhibited pH-dependent proteolytic activity. Quantitative real-time PCR showed that PaCTSD mRNA was expressed in all detected tissues in healthy sweetfish. The highest expression was observed in the spleen and white blood cells, followed by liver, head-kidney, kidney, intestine, gill, and muscle. After Listonella anguillarum infection, PaCTSD transcripts were up-regulated significantly in liver, spleen, white blood cells, and head-kidney of sweetfish. In summary, PaCTSD has proteolytic activity and is closely involved in the immune response of sweetfish. |
format |
Text |
author |
Yu, JIAO Chang-Hong, LI Xin-Jiang, LU Jiong, CHEN |
author_facet |
Yu, JIAO Chang-Hong, LI Xin-Jiang, LU Jiong, CHEN |
author_sort |
Yu, JIAO |
title |
Characterization and expression of sweetfish (Plecoglossus altivelis) cathepsin D |
title_short |
Characterization and expression of sweetfish (Plecoglossus altivelis) cathepsin D |
title_full |
Characterization and expression of sweetfish (Plecoglossus altivelis) cathepsin D |
title_fullStr |
Characterization and expression of sweetfish (Plecoglossus altivelis) cathepsin D |
title_full_unstemmed |
Characterization and expression of sweetfish (Plecoglossus altivelis) cathepsin D |
title_sort |
characterization and expression of sweetfish (plecoglossus altivelis) cathepsin d |
publisher |
Science Press |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031676/ http://www.ncbi.nlm.nih.gov/pubmed/25017748 https://doi.org/10.13918/j.issn.2095-8137.2014.4.294 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031676/ http://www.ncbi.nlm.nih.gov/pubmed/25017748 http://dx.doi.org/10.13918/j.issn.2095-8137.2014.4.294 |
op_rights |
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.13918/j.issn.2095-8137.2014.4.294 |
_version_ |
1766362995263275008 |