Multilocus Sequence Analysis of Close Relatives Vibrio anguillarum and Vibrio ordalii
The genetic heterogeneity of the close relatives Vibrio anguillarum and Vibrio ordalii, both serious pathogens of fish causing extensive losses in aquaculture, was studied. Eight housekeeping genes, i.e., atpA, ftsZ, gapA, gyrB, mreB, rpoA, topA, and pyrH, were partially sequenced in 116 isolates fr...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007779/ http://www.ncbi.nlm.nih.gov/pubmed/27371582 https://doi.org/10.1128/AEM.00620-16 |
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ftpubmed:oai:pubmedcentral.nih.gov:5007779 2023-05-15T15:27:33+02:00 Multilocus Sequence Analysis of Close Relatives Vibrio anguillarum and Vibrio ordalii Steinum, Terje M. Karataş, Süheyla Martinussen, Nora Tandstad Meirelles, Pedro M. Thompson, Fabiano L. Colquhoun, Duncan J. 2016-08-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007779/ http://www.ncbi.nlm.nih.gov/pubmed/27371582 https://doi.org/10.1128/AEM.00620-16 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007779/ http://www.ncbi.nlm.nih.gov/pubmed/27371582 http://dx.doi.org/10.1128/AEM.00620-16 Copyright © 2016, American Society for Microbiology. All Rights Reserved. Genetics and Molecular Biology Text 2016 ftpubmed https://doi.org/10.1128/AEM.00620-16 2017-03-05T01:15:05Z The genetic heterogeneity of the close relatives Vibrio anguillarum and Vibrio ordalii, both serious pathogens of fish causing extensive losses in aquaculture, was studied. Eight housekeeping genes, i.e., atpA, ftsZ, gapA, gyrB, mreB, rpoA, topA, and pyrH, were partially sequenced in 116 isolates from diverse fish species and geographical areas. The eight genes appear to be under purifying selection, and the genetic diversity in the total data set was estimated to be 0.767 ± 0.026. Our multilocus sequence analysis (MLSA) scheme identified several widespread clonal complexes and resolved the isolates, for the most part, according to serotype. Serotype O2b isolates from diseased cod in Norway, Ireland, and Scotland were found to be extremely homogeneous. Horizontal gene transfer appears to be fairly common within and between clonal complexes. Taken together, MLSA and in silico DNA-DNA hybridization (DDH) calculations suggest that some isolates previously characterized as V. ordalii, i.e., 12B09, FF93, FS144, and FS238, are in fact V. anguillarum isolates. The precise taxonomic situation for two isolates from Atlantic cod that display several traits consistent with V. ordalii, i.e., NVI 5286 and NVI 5918, and a single environmental strain that was previously considered to represent V. ordalii, i.e., FF167, is less clear. Text atlantic cod PubMed Central (PMC) Norway Applied and Environmental Microbiology 82 18 5496 5504 |
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Genetics and Molecular Biology |
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Genetics and Molecular Biology Steinum, Terje M. Karataş, Süheyla Martinussen, Nora Tandstad Meirelles, Pedro M. Thompson, Fabiano L. Colquhoun, Duncan J. Multilocus Sequence Analysis of Close Relatives Vibrio anguillarum and Vibrio ordalii |
topic_facet |
Genetics and Molecular Biology |
description |
The genetic heterogeneity of the close relatives Vibrio anguillarum and Vibrio ordalii, both serious pathogens of fish causing extensive losses in aquaculture, was studied. Eight housekeeping genes, i.e., atpA, ftsZ, gapA, gyrB, mreB, rpoA, topA, and pyrH, were partially sequenced in 116 isolates from diverse fish species and geographical areas. The eight genes appear to be under purifying selection, and the genetic diversity in the total data set was estimated to be 0.767 ± 0.026. Our multilocus sequence analysis (MLSA) scheme identified several widespread clonal complexes and resolved the isolates, for the most part, according to serotype. Serotype O2b isolates from diseased cod in Norway, Ireland, and Scotland were found to be extremely homogeneous. Horizontal gene transfer appears to be fairly common within and between clonal complexes. Taken together, MLSA and in silico DNA-DNA hybridization (DDH) calculations suggest that some isolates previously characterized as V. ordalii, i.e., 12B09, FF93, FS144, and FS238, are in fact V. anguillarum isolates. The precise taxonomic situation for two isolates from Atlantic cod that display several traits consistent with V. ordalii, i.e., NVI 5286 and NVI 5918, and a single environmental strain that was previously considered to represent V. ordalii, i.e., FF167, is less clear. |
format |
Text |
author |
Steinum, Terje M. Karataş, Süheyla Martinussen, Nora Tandstad Meirelles, Pedro M. Thompson, Fabiano L. Colquhoun, Duncan J. |
author_facet |
Steinum, Terje M. Karataş, Süheyla Martinussen, Nora Tandstad Meirelles, Pedro M. Thompson, Fabiano L. Colquhoun, Duncan J. |
author_sort |
Steinum, Terje M. |
title |
Multilocus Sequence Analysis of Close Relatives Vibrio anguillarum and Vibrio ordalii |
title_short |
Multilocus Sequence Analysis of Close Relatives Vibrio anguillarum and Vibrio ordalii |
title_full |
Multilocus Sequence Analysis of Close Relatives Vibrio anguillarum and Vibrio ordalii |
title_fullStr |
Multilocus Sequence Analysis of Close Relatives Vibrio anguillarum and Vibrio ordalii |
title_full_unstemmed |
Multilocus Sequence Analysis of Close Relatives Vibrio anguillarum and Vibrio ordalii |
title_sort |
multilocus sequence analysis of close relatives vibrio anguillarum and vibrio ordalii |
publisher |
American Society for Microbiology |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007779/ http://www.ncbi.nlm.nih.gov/pubmed/27371582 https://doi.org/10.1128/AEM.00620-16 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007779/ http://www.ncbi.nlm.nih.gov/pubmed/27371582 http://dx.doi.org/10.1128/AEM.00620-16 |
op_rights |
Copyright © 2016, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/AEM.00620-16 |
container_title |
Applied and Environmental Microbiology |
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82 |
container_issue |
18 |
container_start_page |
5496 |
op_container_end_page |
5504 |
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1766357980832333824 |