Spatial and Temporal Dynamics of Pacific Oyster Hemolymph Microbiota across Multiple Scales
Unveiling the factors and processes that shape the dynamics of host associated microbial communities (microbiota) under natural conditions is an important part of understanding and predicting an organism's response to a changing environment. The microbiota is shaped by host (i.e., genetic) fact...
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ftpubmed:oai:pubmedcentral.nih.gov:5006416 2023-05-15T17:54:20+02:00 Spatial and Temporal Dynamics of Pacific Oyster Hemolymph Microbiota across Multiple Scales Lokmer, Ana Goedknegt, M. Anouk Thieltges, David W. Fiorentino, Dario Kuenzel, Sven Baines, John F. Wegner, K. Mathias 2016-08-31 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006416/ https://doi.org/10.3389/fmicb.2016.01367 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006416/ http://dx.doi.org/10.3389/fmicb.2016.01367 Copyright © 2016 Lokmer, Goedknegt, Thieltges, Fiorentino, Kuenzel, Baines and Wegner. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2016 ftpubmed https://doi.org/10.3389/fmicb.2016.01367 2016-09-18T00:09:08Z Unveiling the factors and processes that shape the dynamics of host associated microbial communities (microbiota) under natural conditions is an important part of understanding and predicting an organism's response to a changing environment. The microbiota is shaped by host (i.e., genetic) factors as well as by the biotic and abiotic environment. Studying natural variation of microbial community composition in multiple host genetic backgrounds across spatial as well as temporal scales represents a means to untangle this complex interplay. Here, we combined a spatially-stratified with a longitudinal sampling scheme within differentiated host genetic backgrounds by reciprocally transplanting Pacific oysters between two sites in the Wadden Sea (Sylt and Texel). To further differentiate contingent site from host genetic effects, we repeatedly sampled the same individuals over a summer season to examine structure, diversity and dynamics of individual hemolymph microbiota following experimental removal of resident microbiota by antibiotic treatment. While a large proportion of microbiome variation could be attributed to immediate environmental conditions, we observed persistent effects of antibiotic treatment and translocation suggesting that hemolymph microbial community dynamics is subject to within-microbiome interactions and host population specific factors. In addition, the analysis of spatial variation revealed that the within-site microenvironmental heterogeneity resulted in high small-scale variability, as opposed to large-scale (between-site) stability. Similarly, considerable within-individual temporal variability was in contrast with the overall temporal stability at the site level. Overall, our longitudinal, spatially-stratified sampling design revealed that variation in hemolymph microbiota is strongly influenced by site and immediate environmental conditions, whereas internal microbiome dynamics and oyster-related factors add to their long-term stability. The combination of small and large scale ... Text Pacific oyster PubMed Central (PMC) Pacific Frontiers in Microbiology 7 |
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Microbiology Lokmer, Ana Goedknegt, M. Anouk Thieltges, David W. Fiorentino, Dario Kuenzel, Sven Baines, John F. Wegner, K. Mathias Spatial and Temporal Dynamics of Pacific Oyster Hemolymph Microbiota across Multiple Scales |
topic_facet |
Microbiology |
description |
Unveiling the factors and processes that shape the dynamics of host associated microbial communities (microbiota) under natural conditions is an important part of understanding and predicting an organism's response to a changing environment. The microbiota is shaped by host (i.e., genetic) factors as well as by the biotic and abiotic environment. Studying natural variation of microbial community composition in multiple host genetic backgrounds across spatial as well as temporal scales represents a means to untangle this complex interplay. Here, we combined a spatially-stratified with a longitudinal sampling scheme within differentiated host genetic backgrounds by reciprocally transplanting Pacific oysters between two sites in the Wadden Sea (Sylt and Texel). To further differentiate contingent site from host genetic effects, we repeatedly sampled the same individuals over a summer season to examine structure, diversity and dynamics of individual hemolymph microbiota following experimental removal of resident microbiota by antibiotic treatment. While a large proportion of microbiome variation could be attributed to immediate environmental conditions, we observed persistent effects of antibiotic treatment and translocation suggesting that hemolymph microbial community dynamics is subject to within-microbiome interactions and host population specific factors. In addition, the analysis of spatial variation revealed that the within-site microenvironmental heterogeneity resulted in high small-scale variability, as opposed to large-scale (between-site) stability. Similarly, considerable within-individual temporal variability was in contrast with the overall temporal stability at the site level. Overall, our longitudinal, spatially-stratified sampling design revealed that variation in hemolymph microbiota is strongly influenced by site and immediate environmental conditions, whereas internal microbiome dynamics and oyster-related factors add to their long-term stability. The combination of small and large scale ... |
format |
Text |
author |
Lokmer, Ana Goedknegt, M. Anouk Thieltges, David W. Fiorentino, Dario Kuenzel, Sven Baines, John F. Wegner, K. Mathias |
author_facet |
Lokmer, Ana Goedknegt, M. Anouk Thieltges, David W. Fiorentino, Dario Kuenzel, Sven Baines, John F. Wegner, K. Mathias |
author_sort |
Lokmer, Ana |
title |
Spatial and Temporal Dynamics of Pacific Oyster Hemolymph Microbiota across Multiple Scales |
title_short |
Spatial and Temporal Dynamics of Pacific Oyster Hemolymph Microbiota across Multiple Scales |
title_full |
Spatial and Temporal Dynamics of Pacific Oyster Hemolymph Microbiota across Multiple Scales |
title_fullStr |
Spatial and Temporal Dynamics of Pacific Oyster Hemolymph Microbiota across Multiple Scales |
title_full_unstemmed |
Spatial and Temporal Dynamics of Pacific Oyster Hemolymph Microbiota across Multiple Scales |
title_sort |
spatial and temporal dynamics of pacific oyster hemolymph microbiota across multiple scales |
publisher |
Frontiers Media S.A. |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006416/ https://doi.org/10.3389/fmicb.2016.01367 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006416/ http://dx.doi.org/10.3389/fmicb.2016.01367 |
op_rights |
Copyright © 2016 Lokmer, Goedknegt, Thieltges, Fiorentino, Kuenzel, Baines and Wegner. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2016.01367 |
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Frontiers in Microbiology |
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7 |
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1766162090452582400 |