Secondary Metabolites in Ramalina terebrata Detected by UHPLC/ESI/MS/MS and Identification of Parietin as Tau Protein Inhibitor
Liquid chromatography coupled with mass spectrometry is an outstanding methodology for fast analysis of phenolic compounds in biological samples. Twenty two compounds were quickly and accurately identified in the methanolic extract of the Antarctic lichen Ramalina terebrata for the first time using...
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ftpubmed:oai:pubmedcentral.nih.gov:5000700 2023-05-15T13:56:16+02:00 Secondary Metabolites in Ramalina terebrata Detected by UHPLC/ESI/MS/MS and Identification of Parietin as Tau Protein Inhibitor Cornejo, Alberto Salgado, Francisco Caballero, Julio Vargas, Reinaldo Simirgiotis, Mario Areche, Carlos 2016-08-18 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000700/ http://www.ncbi.nlm.nih.gov/pubmed/27548142 https://doi.org/10.3390/ijms17081303 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000700/ http://www.ncbi.nlm.nih.gov/pubmed/27548142 http://dx.doi.org/10.3390/ijms17081303 © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2016 ftpubmed https://doi.org/10.3390/ijms17081303 2016-09-04T00:33:00Z Liquid chromatography coupled with mass spectrometry is an outstanding methodology for fast analysis of phenolic compounds in biological samples. Twenty two compounds were quickly and accurately identified in the methanolic extract of the Antarctic lichen Ramalina terebrata for the first time using ultra high pressure liquid chromatography coupled with photodiode array detector and high resolution mass spectrometry (UHPLC-PDA-Q/Orbitrap/MS/MS). In addition, the extract and the four compounds isolated from this species were tested for the inhibitory activity of tau protein aggregation, which is a protein involved in Alzheimer’s disease (AD). All compounds showed null activity with the exception of parietin, which it was able to inhibit aggregation process of tau in a concentration range between 3 µg/mL (10 µM) to 28 µg/mL (100 µM). In addition, we show how parietin interact with tau 306VQIVYK311 hexapeptide inside of the microtubule binding domain (4R) with the help of molecular docking experiments. Finally, the constituents present in the methanolic extract could possibly contribute to the established anti-aggregation activity for this extract and this in-depth analysis of the chemical composition of R. terebrata could guide further research into its medicinal properties and potential uses. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic International Journal of Molecular Sciences 17 8 1303 |
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Article Cornejo, Alberto Salgado, Francisco Caballero, Julio Vargas, Reinaldo Simirgiotis, Mario Areche, Carlos Secondary Metabolites in Ramalina terebrata Detected by UHPLC/ESI/MS/MS and Identification of Parietin as Tau Protein Inhibitor |
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Liquid chromatography coupled with mass spectrometry is an outstanding methodology for fast analysis of phenolic compounds in biological samples. Twenty two compounds were quickly and accurately identified in the methanolic extract of the Antarctic lichen Ramalina terebrata for the first time using ultra high pressure liquid chromatography coupled with photodiode array detector and high resolution mass spectrometry (UHPLC-PDA-Q/Orbitrap/MS/MS). In addition, the extract and the four compounds isolated from this species were tested for the inhibitory activity of tau protein aggregation, which is a protein involved in Alzheimer’s disease (AD). All compounds showed null activity with the exception of parietin, which it was able to inhibit aggregation process of tau in a concentration range between 3 µg/mL (10 µM) to 28 µg/mL (100 µM). In addition, we show how parietin interact with tau 306VQIVYK311 hexapeptide inside of the microtubule binding domain (4R) with the help of molecular docking experiments. Finally, the constituents present in the methanolic extract could possibly contribute to the established anti-aggregation activity for this extract and this in-depth analysis of the chemical composition of R. terebrata could guide further research into its medicinal properties and potential uses. |
format |
Text |
author |
Cornejo, Alberto Salgado, Francisco Caballero, Julio Vargas, Reinaldo Simirgiotis, Mario Areche, Carlos |
author_facet |
Cornejo, Alberto Salgado, Francisco Caballero, Julio Vargas, Reinaldo Simirgiotis, Mario Areche, Carlos |
author_sort |
Cornejo, Alberto |
title |
Secondary Metabolites in Ramalina terebrata Detected by UHPLC/ESI/MS/MS and Identification of Parietin as Tau Protein Inhibitor |
title_short |
Secondary Metabolites in Ramalina terebrata Detected by UHPLC/ESI/MS/MS and Identification of Parietin as Tau Protein Inhibitor |
title_full |
Secondary Metabolites in Ramalina terebrata Detected by UHPLC/ESI/MS/MS and Identification of Parietin as Tau Protein Inhibitor |
title_fullStr |
Secondary Metabolites in Ramalina terebrata Detected by UHPLC/ESI/MS/MS and Identification of Parietin as Tau Protein Inhibitor |
title_full_unstemmed |
Secondary Metabolites in Ramalina terebrata Detected by UHPLC/ESI/MS/MS and Identification of Parietin as Tau Protein Inhibitor |
title_sort |
secondary metabolites in ramalina terebrata detected by uhplc/esi/ms/ms and identification of parietin as tau protein inhibitor |
publisher |
MDPI |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000700/ http://www.ncbi.nlm.nih.gov/pubmed/27548142 https://doi.org/10.3390/ijms17081303 |
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Antarctic The Antarctic |
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Antarctic The Antarctic |
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Antarc* Antarctic |
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Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000700/ http://www.ncbi.nlm.nih.gov/pubmed/27548142 http://dx.doi.org/10.3390/ijms17081303 |
op_rights |
© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/ijms17081303 |
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International Journal of Molecular Sciences |
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17 |
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8 |
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1303 |
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1766263635240288256 |