Molecular Cloning and Functional Expression of a Δ9- Fatty Acid Desaturase from an Antarctic Pseudomonas sp. A3
Fatty acid desaturase enzymes play an essential role in the synthesis of unsaturated fatty acids. Pseudomonas sp. A3 was found to produce a large amount of palmitoleic and oleic acids after incubation at low temperatures. Using polymerase Chain Reaction (PCR), a novel Δ9- fatty acid desaturase gene...
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ftpubmed:oai:pubmedcentral.nih.gov:4975390 2023-05-15T13:40:38+02:00 Molecular Cloning and Functional Expression of a Δ9- Fatty Acid Desaturase from an Antarctic Pseudomonas sp. A3 Garba, Lawal Mohamad Ali, Mohd Shukuri Oslan, Siti Nurbaya Rahman, Raja Noor Zaliha Raja Abd 2016-08-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4975390/ http://www.ncbi.nlm.nih.gov/pubmed/27494717 https://doi.org/10.1371/journal.pone.0160681 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4975390/ http://www.ncbi.nlm.nih.gov/pubmed/27494717 http://dx.doi.org/10.1371/journal.pone.0160681 © 2016 Garba et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2016 ftpubmed https://doi.org/10.1371/journal.pone.0160681 2016-08-28T00:04:34Z Fatty acid desaturase enzymes play an essential role in the synthesis of unsaturated fatty acids. Pseudomonas sp. A3 was found to produce a large amount of palmitoleic and oleic acids after incubation at low temperatures. Using polymerase Chain Reaction (PCR), a novel Δ9- fatty acid desaturase gene was isolated, cloned, and successfully expressed in Escherichia coli. The gene was designated as PA3FAD9 and has an open reading frame of 1,185 bp which codes for 394 amino acids with a predicted molecular weight of 45 kDa. The activity of the gene product was confirmed via GCMS, which showed a functional putative Δ9-fatty acid desaturase capable of increasing the total amount of cellular unsaturated fatty acids of the E. coli cells expressing the gene. The results demonstrate that the cellular palmitoleic acids have increased two-fold upon expression at 15°C using only 0.1 mM IPTG. Therefore, PA3FAD9 from Pseudomonas sp.A3 codes for a Δ9-fatty acid desaturase-like protein which was actively expressed in E. coli. Text Antarc* Antarctic PubMed Central (PMC) Antarctic PLOS ONE 11 8 e0160681 |
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Research Article Garba, Lawal Mohamad Ali, Mohd Shukuri Oslan, Siti Nurbaya Rahman, Raja Noor Zaliha Raja Abd Molecular Cloning and Functional Expression of a Δ9- Fatty Acid Desaturase from an Antarctic Pseudomonas sp. A3 |
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Research Article |
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Fatty acid desaturase enzymes play an essential role in the synthesis of unsaturated fatty acids. Pseudomonas sp. A3 was found to produce a large amount of palmitoleic and oleic acids after incubation at low temperatures. Using polymerase Chain Reaction (PCR), a novel Δ9- fatty acid desaturase gene was isolated, cloned, and successfully expressed in Escherichia coli. The gene was designated as PA3FAD9 and has an open reading frame of 1,185 bp which codes for 394 amino acids with a predicted molecular weight of 45 kDa. The activity of the gene product was confirmed via GCMS, which showed a functional putative Δ9-fatty acid desaturase capable of increasing the total amount of cellular unsaturated fatty acids of the E. coli cells expressing the gene. The results demonstrate that the cellular palmitoleic acids have increased two-fold upon expression at 15°C using only 0.1 mM IPTG. Therefore, PA3FAD9 from Pseudomonas sp.A3 codes for a Δ9-fatty acid desaturase-like protein which was actively expressed in E. coli. |
format |
Text |
author |
Garba, Lawal Mohamad Ali, Mohd Shukuri Oslan, Siti Nurbaya Rahman, Raja Noor Zaliha Raja Abd |
author_facet |
Garba, Lawal Mohamad Ali, Mohd Shukuri Oslan, Siti Nurbaya Rahman, Raja Noor Zaliha Raja Abd |
author_sort |
Garba, Lawal |
title |
Molecular Cloning and Functional Expression of a Δ9- Fatty Acid Desaturase from an Antarctic Pseudomonas sp. A3 |
title_short |
Molecular Cloning and Functional Expression of a Δ9- Fatty Acid Desaturase from an Antarctic Pseudomonas sp. A3 |
title_full |
Molecular Cloning and Functional Expression of a Δ9- Fatty Acid Desaturase from an Antarctic Pseudomonas sp. A3 |
title_fullStr |
Molecular Cloning and Functional Expression of a Δ9- Fatty Acid Desaturase from an Antarctic Pseudomonas sp. A3 |
title_full_unstemmed |
Molecular Cloning and Functional Expression of a Δ9- Fatty Acid Desaturase from an Antarctic Pseudomonas sp. A3 |
title_sort |
molecular cloning and functional expression of a δ9- fatty acid desaturase from an antarctic pseudomonas sp. a3 |
publisher |
Public Library of Science |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4975390/ http://www.ncbi.nlm.nih.gov/pubmed/27494717 https://doi.org/10.1371/journal.pone.0160681 |
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Antarctic |
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Antarctic |
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Antarc* Antarctic |
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Antarc* Antarctic |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4975390/ http://www.ncbi.nlm.nih.gov/pubmed/27494717 http://dx.doi.org/10.1371/journal.pone.0160681 |
op_rights |
© 2016 Garba et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
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https://doi.org/10.1371/journal.pone.0160681 |
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PLOS ONE |
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e0160681 |
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