A high-resolution map of the gut microbiota in Atlantic salmon (Salmo salar): A basis for comparative gut microbial research
Gut health challenges, possibly related to alterations in gut microbiota, caused by plant ingredients in the diets, cause losses in Atlantic salmon production. To investigate the role of the microbiota for gut function and health, detailed characterization of the gut microbiota is needed. We present...
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ftpubmed:oai:pubmedcentral.nih.gov:4971465 2023-05-15T15:30:28+02:00 A high-resolution map of the gut microbiota in Atlantic salmon (Salmo salar): A basis for comparative gut microbial research Gajardo, Karina Rodiles, Ana Kortner, Trond M. Krogdahl, Åshild Bakke, Anne Marie Merrifield, Daniel L. Sørum, Henning 2016-08-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971465/ http://www.ncbi.nlm.nih.gov/pubmed/27485205 https://doi.org/10.1038/srep30893 en eng Nature Publishing Group http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971465/ http://www.ncbi.nlm.nih.gov/pubmed/27485205 http://dx.doi.org/10.1038/srep30893 Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ CC-BY Article Text 2016 ftpubmed https://doi.org/10.1038/srep30893 2016-08-14T00:13:31Z Gut health challenges, possibly related to alterations in gut microbiota, caused by plant ingredients in the diets, cause losses in Atlantic salmon production. To investigate the role of the microbiota for gut function and health, detailed characterization of the gut microbiota is needed. We present the first in-depth characterization of salmon gut microbiota based on high-throughput sequencing of the 16S rRNA gene’s V1-V2 region. Samples were taken from five intestinal compartments: digesta from proximal, mid and distal intestine and of mucosa from mid and distal intestine of 67.3 g salmon kept in seawater (12–14 °C) and fed a commercial diet for 4 weeks. Microbial richness and diversity differed significantly and were higher in the digesta than the mucosa. In mucosa, Proteobacteria dominated the microbiota (90%), whereas in digesta both Proteobacteria (47%) and Firmicutes (38%) showed high abundance. Future studies of diet and environmental impacts on gut microbiota should therefore differentiate between effects on mucosa and digesta in the proximal, mid and the distal intestine. A core microbiota, represented by 22 OTUs, was found in 80% of the samples. The gut microbiota of Atlantic salmon showed similarities with that of mammals. Text Atlantic salmon Salmo salar PubMed Central (PMC) Scientific Reports 6 1 |
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Article Gajardo, Karina Rodiles, Ana Kortner, Trond M. Krogdahl, Åshild Bakke, Anne Marie Merrifield, Daniel L. Sørum, Henning A high-resolution map of the gut microbiota in Atlantic salmon (Salmo salar): A basis for comparative gut microbial research |
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Article |
description |
Gut health challenges, possibly related to alterations in gut microbiota, caused by plant ingredients in the diets, cause losses in Atlantic salmon production. To investigate the role of the microbiota for gut function and health, detailed characterization of the gut microbiota is needed. We present the first in-depth characterization of salmon gut microbiota based on high-throughput sequencing of the 16S rRNA gene’s V1-V2 region. Samples were taken from five intestinal compartments: digesta from proximal, mid and distal intestine and of mucosa from mid and distal intestine of 67.3 g salmon kept in seawater (12–14 °C) and fed a commercial diet for 4 weeks. Microbial richness and diversity differed significantly and were higher in the digesta than the mucosa. In mucosa, Proteobacteria dominated the microbiota (90%), whereas in digesta both Proteobacteria (47%) and Firmicutes (38%) showed high abundance. Future studies of diet and environmental impacts on gut microbiota should therefore differentiate between effects on mucosa and digesta in the proximal, mid and the distal intestine. A core microbiota, represented by 22 OTUs, was found in 80% of the samples. The gut microbiota of Atlantic salmon showed similarities with that of mammals. |
format |
Text |
author |
Gajardo, Karina Rodiles, Ana Kortner, Trond M. Krogdahl, Åshild Bakke, Anne Marie Merrifield, Daniel L. Sørum, Henning |
author_facet |
Gajardo, Karina Rodiles, Ana Kortner, Trond M. Krogdahl, Åshild Bakke, Anne Marie Merrifield, Daniel L. Sørum, Henning |
author_sort |
Gajardo, Karina |
title |
A high-resolution map of the gut microbiota in Atlantic salmon (Salmo salar): A basis for comparative gut microbial research |
title_short |
A high-resolution map of the gut microbiota in Atlantic salmon (Salmo salar): A basis for comparative gut microbial research |
title_full |
A high-resolution map of the gut microbiota in Atlantic salmon (Salmo salar): A basis for comparative gut microbial research |
title_fullStr |
A high-resolution map of the gut microbiota in Atlantic salmon (Salmo salar): A basis for comparative gut microbial research |
title_full_unstemmed |
A high-resolution map of the gut microbiota in Atlantic salmon (Salmo salar): A basis for comparative gut microbial research |
title_sort |
high-resolution map of the gut microbiota in atlantic salmon (salmo salar): a basis for comparative gut microbial research |
publisher |
Nature Publishing Group |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971465/ http://www.ncbi.nlm.nih.gov/pubmed/27485205 https://doi.org/10.1038/srep30893 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971465/ http://www.ncbi.nlm.nih.gov/pubmed/27485205 http://dx.doi.org/10.1038/srep30893 |
op_rights |
Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
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CC-BY |
op_doi |
https://doi.org/10.1038/srep30893 |
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Scientific Reports |
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6 |
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1 |
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1766360909326843904 |