Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis
Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for el...
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ftpubmed:oai:pubmedcentral.nih.gov:4968460 2023-05-15T13:40:38+02:00 Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis Tsuji, Masaharu 2016-07-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968460/ https://doi.org/10.1098/rsos.160106 en eng The Royal Society http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968460/ http://dx.doi.org/10.1098/rsos.160106 © 2016 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. CC-BY Biology (Whole Organism) Text 2016 ftpubmed https://doi.org/10.1098/rsos.160106 2016-08-07T00:26:20Z Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for elucidating polar-region ecosystems. Here, I report that two strains of the Antarctic basidiomycetous yeast Mrakia blollopis exhibited distinct growth characteristics under subzero conditions: SK-4 grew efficiently, whereas TKG1-2 did not. I analysed the metabolite responses elicited by cold stress in these two M. blollopis strains by using capillary electrophoresis–time-of-flight mass spectrometry. M. blollopis SK-4, which grew well under subzero temperatures, accumulated high levels of TCA-cycle metabolites, lactic acid, aromatic amino acids and polyamines in response to cold shock. Polyamines are recognized to function in cell-growth and developmental processes, and aromatic amino acids are also known to improve cell growth at low temperatures. By contrast, in TKG1-2, which did not grow efficiently, cold stress strongly induced the metabolites of the TCA cycle, but other metabolites were not highly accumulated in the cell. Thus, these differences in metabolite responses could contribute to the distinct abilities of SK-4 and TKG1-2 cells to grow under subzero temperature conditions. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Royal Society Open Science 3 7 160106 |
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Biology (Whole Organism) Tsuji, Masaharu Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
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Biology (Whole Organism) |
description |
Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for elucidating polar-region ecosystems. Here, I report that two strains of the Antarctic basidiomycetous yeast Mrakia blollopis exhibited distinct growth characteristics under subzero conditions: SK-4 grew efficiently, whereas TKG1-2 did not. I analysed the metabolite responses elicited by cold stress in these two M. blollopis strains by using capillary electrophoresis–time-of-flight mass spectrometry. M. blollopis SK-4, which grew well under subzero temperatures, accumulated high levels of TCA-cycle metabolites, lactic acid, aromatic amino acids and polyamines in response to cold shock. Polyamines are recognized to function in cell-growth and developmental processes, and aromatic amino acids are also known to improve cell growth at low temperatures. By contrast, in TKG1-2, which did not grow efficiently, cold stress strongly induced the metabolites of the TCA cycle, but other metabolites were not highly accumulated in the cell. Thus, these differences in metabolite responses could contribute to the distinct abilities of SK-4 and TKG1-2 cells to grow under subzero temperature conditions. |
format |
Text |
author |
Tsuji, Masaharu |
author_facet |
Tsuji, Masaharu |
author_sort |
Tsuji, Masaharu |
title |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_short |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_full |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_fullStr |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_full_unstemmed |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_sort |
cold-stress responses in the antarctic basidiomycetous yeast mrakia blollopis |
publisher |
The Royal Society |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968460/ https://doi.org/10.1098/rsos.160106 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968460/ http://dx.doi.org/10.1098/rsos.160106 |
op_rights |
© 2016 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1098/rsos.160106 |
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Royal Society Open Science |
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3 |
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7 |
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160106 |
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1766137726010130432 |