Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections
Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are con...
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ftpubmed:oai:pubmedcentral.nih.gov:4955271 2023-05-15T15:58:06+02:00 Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections Green, Timothy J. Vergnes, Agnes Montagnani, Caroline de Lorgeril, Julien 2016-07-21 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4955271/ https://doi.org/10.1186/s13567-016-0356-7 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4955271/ http://dx.doi.org/10.1186/s13567-016-0356-7 © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. CC0 PDM CC-BY Research Article Text 2016 ftpubmed https://doi.org/10.1186/s13567-016-0356-7 2016-07-24T00:20:15Z Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oyster’s microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host. Text Crassostrea gigas PubMed Central (PMC) Pacific Veterinary Research 47 1 |
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Research Article Green, Timothy J. Vergnes, Agnes Montagnani, Caroline de Lorgeril, Julien Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
topic_facet |
Research Article |
description |
Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oyster’s microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host. |
format |
Text |
author |
Green, Timothy J. Vergnes, Agnes Montagnani, Caroline de Lorgeril, Julien |
author_facet |
Green, Timothy J. Vergnes, Agnes Montagnani, Caroline de Lorgeril, Julien |
author_sort |
Green, Timothy J. |
title |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_short |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_full |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_fullStr |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_full_unstemmed |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_sort |
distinct immune responses of juvenile and adult oysters (crassostrea gigas) to viral and bacterial infections |
publisher |
BioMed Central |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4955271/ https://doi.org/10.1186/s13567-016-0356-7 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4955271/ http://dx.doi.org/10.1186/s13567-016-0356-7 |
op_rights |
© The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
op_rightsnorm |
CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s13567-016-0356-7 |
container_title |
Veterinary Research |
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47 |
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1 |
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1766393824339296256 |