Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense
The effect of the dinoflagellate, Alexandrium fundyense, on relative expression of glutathione S-transferase (GST) transcripts was examined in the copepod Calanus finmarchicus. Adult females were fed for 5-days on one of three experimental diets: control (100% Rhodomonas spp.), low dose of A. fundye...
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ftpubmed:oai:pubmedcentral.nih.gov:4948837 2023-05-15T15:47:56+02:00 Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense Roncalli, Vittoria Jungbluth, Michelle J. Lenz, Petra H. 2016-07-18 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948837/ http://www.ncbi.nlm.nih.gov/pubmed/27427938 https://doi.org/10.1371/journal.pone.0159563 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948837/ http://www.ncbi.nlm.nih.gov/pubmed/27427938 http://dx.doi.org/10.1371/journal.pone.0159563 © 2016 Roncalli et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2016 ftpubmed https://doi.org/10.1371/journal.pone.0159563 2016-08-07T00:17:43Z The effect of the dinoflagellate, Alexandrium fundyense, on relative expression of glutathione S-transferase (GST) transcripts was examined in the copepod Calanus finmarchicus. Adult females were fed for 5-days on one of three experimental diets: control (100% Rhodomonas spp.), low dose of A. fundyense (25% by volume, 75% Rhodomonas spp.), and high dose (100% A. fundyense). Relative expression of three GST genes was measured using RT-qPCR on days 0.5, 1, 2 and 5 in two independent experiments. Differential regulation was found for the Delta and the Sigma GSTs between 0.5 to 2 days, but not on day 5 in both experiments. The third GST, a microsomal, was not differentially expressed in either treatment or day. RT-qPCR results from the two experiments were similar, even though experimental females were collected from the Gulf of Maine on different dates and their reproductive output differed. In the second experiment, expression of 39 GSTs was determined on days 2 and 5 using RNA-Seq. Global gene expression analyses agreed with the RT-qPCR results. Furthermore, the RNA-Seq measurements indicated that only four GSTs were differentially expressed under the experimental conditions, and the response was small in amplitude. In summary, the A. fundyense diet led to a rapid and transient response in C. finmarchicus in three cytosolic GSTs, while a fourth GST (Omega I) was significantly up-regulated on day 5. Although there was some regulation of GSTs in response the toxic dinoflagellate, the tolerance to A. fundyense by C. finmarchicus is not dependent on the long-term up-regulation of specific GSTs. Text Calanus finmarchicus PubMed Central (PMC) PLOS ONE 11 7 e0159563 |
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Research Article Roncalli, Vittoria Jungbluth, Michelle J. Lenz, Petra H. Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense |
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description |
The effect of the dinoflagellate, Alexandrium fundyense, on relative expression of glutathione S-transferase (GST) transcripts was examined in the copepod Calanus finmarchicus. Adult females were fed for 5-days on one of three experimental diets: control (100% Rhodomonas spp.), low dose of A. fundyense (25% by volume, 75% Rhodomonas spp.), and high dose (100% A. fundyense). Relative expression of three GST genes was measured using RT-qPCR on days 0.5, 1, 2 and 5 in two independent experiments. Differential regulation was found for the Delta and the Sigma GSTs between 0.5 to 2 days, but not on day 5 in both experiments. The third GST, a microsomal, was not differentially expressed in either treatment or day. RT-qPCR results from the two experiments were similar, even though experimental females were collected from the Gulf of Maine on different dates and their reproductive output differed. In the second experiment, expression of 39 GSTs was determined on days 2 and 5 using RNA-Seq. Global gene expression analyses agreed with the RT-qPCR results. Furthermore, the RNA-Seq measurements indicated that only four GSTs were differentially expressed under the experimental conditions, and the response was small in amplitude. In summary, the A. fundyense diet led to a rapid and transient response in C. finmarchicus in three cytosolic GSTs, while a fourth GST (Omega I) was significantly up-regulated on day 5. Although there was some regulation of GSTs in response the toxic dinoflagellate, the tolerance to A. fundyense by C. finmarchicus is not dependent on the long-term up-regulation of specific GSTs. |
format |
Text |
author |
Roncalli, Vittoria Jungbluth, Michelle J. Lenz, Petra H. |
author_facet |
Roncalli, Vittoria Jungbluth, Michelle J. Lenz, Petra H. |
author_sort |
Roncalli, Vittoria |
title |
Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense |
title_short |
Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense |
title_full |
Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense |
title_fullStr |
Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense |
title_full_unstemmed |
Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense |
title_sort |
glutathione s-transferase regulation in calanus finmarchicus feeding on the toxic dinoflagellate alexandrium fundyense |
publisher |
Public Library of Science |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948837/ http://www.ncbi.nlm.nih.gov/pubmed/27427938 https://doi.org/10.1371/journal.pone.0159563 |
genre |
Calanus finmarchicus |
genre_facet |
Calanus finmarchicus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948837/ http://www.ncbi.nlm.nih.gov/pubmed/27427938 http://dx.doi.org/10.1371/journal.pone.0159563 |
op_rights |
© 2016 Roncalli et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
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https://doi.org/10.1371/journal.pone.0159563 |
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PLOS ONE |
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11 |
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7 |
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e0159563 |
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