TWO NEW SPECIES OF SARCOCYSTIS (APICOMPLEXA: SARCOCYSTIDAE) INFECTING THE WOLVERINE (GULO GULO) FROM NUNAVUT, CANADA
Infection with Sarcocystis species is common in many species of animals, but it has not yet been reported in wolverines (Gulo gulo). Histological sections of tongues from 41 wolverines in the Kitikmeot Region, Nunavut, Canada, were examined for sarcocysts. Sarcocysts were found in 33 (80.4%) wolveri...
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ftpubmed:oai:pubmedcentral.nih.gov:4907793 2023-05-15T16:32:18+02:00 TWO NEW SPECIES OF SARCOCYSTIS (APICOMPLEXA: SARCOCYSTIDAE) INFECTING THE WOLVERINE (GULO GULO) FROM NUNAVUT, CANADA Dubey, J. P. Reichard, Mason V. Torretti, Luigi Garvon, Jason M. Sundar, N. Grigg, M. E. 2010-04-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4907793/ http://www.ncbi.nlm.nih.gov/pubmed/20950105 https://doi.org/10.1645/GE-2412.1 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4907793/ http://www.ncbi.nlm.nih.gov/pubmed/20950105 http://dx.doi.org/10.1645/GE-2412.1 Article Text 2010 ftpubmed https://doi.org/10.1645/GE-2412.1 2016-06-19T00:19:36Z Infection with Sarcocystis species is common in many species of animals, but it has not yet been reported in wolverines (Gulo gulo). Histological sections of tongues from 41 wolverines in the Kitikmeot Region, Nunavut, Canada, were examined for sarcocysts. Sarcocysts were found in 33 (80.4%) wolverines. Two structurally distinct types of sarcocysts were found. Type A sarcocysts were thin (<1 μm thick) walled. Ultrastructurally, the parasitophorous vacuolar membrane (Pvm) had minute undulations, but it lacked villar protrusions and was not invaginated into the granular layer. The bradyzoites were slender, about 5 × 1 μm in size. Structurally, these sarcocysts were distinct from known species of Sarcocystis and possessed a novel 18S and ITS-1 sequence, sharing 98% and 78% sequence similarity with Sarcocystis canis. A new species name, Sarcocystis kalvikus, is proposed for type A sarcocysts. In contrast, type B sarcocysts had relatively thicker (about 2 μm) cyst walls and larger bradyzoites, each about 10 × 2–3 μm. Ultrastructurally, the Pvm on the sarcocyst wall had villar protrusions that were either mushroom-like or sloping. Molecular analysis identified a unique 18S and ITS-1 sequence that placed them in a clade within the Sarcocystidae. Based on histology, TEM, and genetic data, the new name, Sarcocystis kitikmeotensis, is proposed. Sarcocystis kalvikus was found in 14 (34.1%), S. kitikmeotensis was found in 7 (17%), and both species were found in 12 (29.2%) of 41 wolverines. Text Gulo gulo Kitikmeot Nunavut PubMed Central (PMC) Canada Nunavut Journal of Parasitology 96 5 972 976 |
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Article Dubey, J. P. Reichard, Mason V. Torretti, Luigi Garvon, Jason M. Sundar, N. Grigg, M. E. TWO NEW SPECIES OF SARCOCYSTIS (APICOMPLEXA: SARCOCYSTIDAE) INFECTING THE WOLVERINE (GULO GULO) FROM NUNAVUT, CANADA |
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description |
Infection with Sarcocystis species is common in many species of animals, but it has not yet been reported in wolverines (Gulo gulo). Histological sections of tongues from 41 wolverines in the Kitikmeot Region, Nunavut, Canada, were examined for sarcocysts. Sarcocysts were found in 33 (80.4%) wolverines. Two structurally distinct types of sarcocysts were found. Type A sarcocysts were thin (<1 μm thick) walled. Ultrastructurally, the parasitophorous vacuolar membrane (Pvm) had minute undulations, but it lacked villar protrusions and was not invaginated into the granular layer. The bradyzoites were slender, about 5 × 1 μm in size. Structurally, these sarcocysts were distinct from known species of Sarcocystis and possessed a novel 18S and ITS-1 sequence, sharing 98% and 78% sequence similarity with Sarcocystis canis. A new species name, Sarcocystis kalvikus, is proposed for type A sarcocysts. In contrast, type B sarcocysts had relatively thicker (about 2 μm) cyst walls and larger bradyzoites, each about 10 × 2–3 μm. Ultrastructurally, the Pvm on the sarcocyst wall had villar protrusions that were either mushroom-like or sloping. Molecular analysis identified a unique 18S and ITS-1 sequence that placed them in a clade within the Sarcocystidae. Based on histology, TEM, and genetic data, the new name, Sarcocystis kitikmeotensis, is proposed. Sarcocystis kalvikus was found in 14 (34.1%), S. kitikmeotensis was found in 7 (17%), and both species were found in 12 (29.2%) of 41 wolverines. |
format |
Text |
author |
Dubey, J. P. Reichard, Mason V. Torretti, Luigi Garvon, Jason M. Sundar, N. Grigg, M. E. |
author_facet |
Dubey, J. P. Reichard, Mason V. Torretti, Luigi Garvon, Jason M. Sundar, N. Grigg, M. E. |
author_sort |
Dubey, J. P. |
title |
TWO NEW SPECIES OF SARCOCYSTIS (APICOMPLEXA: SARCOCYSTIDAE) INFECTING THE WOLVERINE (GULO GULO) FROM NUNAVUT, CANADA |
title_short |
TWO NEW SPECIES OF SARCOCYSTIS (APICOMPLEXA: SARCOCYSTIDAE) INFECTING THE WOLVERINE (GULO GULO) FROM NUNAVUT, CANADA |
title_full |
TWO NEW SPECIES OF SARCOCYSTIS (APICOMPLEXA: SARCOCYSTIDAE) INFECTING THE WOLVERINE (GULO GULO) FROM NUNAVUT, CANADA |
title_fullStr |
TWO NEW SPECIES OF SARCOCYSTIS (APICOMPLEXA: SARCOCYSTIDAE) INFECTING THE WOLVERINE (GULO GULO) FROM NUNAVUT, CANADA |
title_full_unstemmed |
TWO NEW SPECIES OF SARCOCYSTIS (APICOMPLEXA: SARCOCYSTIDAE) INFECTING THE WOLVERINE (GULO GULO) FROM NUNAVUT, CANADA |
title_sort |
two new species of sarcocystis (apicomplexa: sarcocystidae) infecting the wolverine (gulo gulo) from nunavut, canada |
publishDate |
2010 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4907793/ http://www.ncbi.nlm.nih.gov/pubmed/20950105 https://doi.org/10.1645/GE-2412.1 |
geographic |
Canada Nunavut |
geographic_facet |
Canada Nunavut |
genre |
Gulo gulo Kitikmeot Nunavut |
genre_facet |
Gulo gulo Kitikmeot Nunavut |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4907793/ http://www.ncbi.nlm.nih.gov/pubmed/20950105 http://dx.doi.org/10.1645/GE-2412.1 |
op_doi |
https://doi.org/10.1645/GE-2412.1 |
container_title |
Journal of Parasitology |
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96 |
container_issue |
5 |
container_start_page |
972 |
op_container_end_page |
976 |
_version_ |
1766022047999197184 |