Formation of lipid bodies and changes in fatty acid composition upon pre-akinete formation in Arctic and Antarctic Zygnema (Zygnematophyceae, Streptophyta) strains
Filamentous green algae of the genus Zygnema (Zygnematophyceae, Streptophyta) are key components of polar hydro-terrestrial mats where they face various stressors including UV irradiation, freezing, desiccation and osmotic stress. Their vegetative cells can develop into pre-akinetes, i.e. reserve-ri...
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ftpubmed:oai:pubmedcentral.nih.gov:4892695 2023-05-15T14:01:25+02:00 Formation of lipid bodies and changes in fatty acid composition upon pre-akinete formation in Arctic and Antarctic Zygnema (Zygnematophyceae, Streptophyta) strains Pichrtová, Martina Arc, Erwann Stöggl, Wolfgang Kranner, Ilse Hájek, Tomáš Hackl, Hubert Holzinger, Andreas 2016-05-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892695/ http://www.ncbi.nlm.nih.gov/pubmed/27170362 https://doi.org/10.1093/femsec/fiw096 en eng Oxford University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892695/ http://www.ncbi.nlm.nih.gov/pubmed/27170362 http://dx.doi.org/10.1093/femsec/fiw096 © FEMS 2016. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Research Article Text 2016 ftpubmed https://doi.org/10.1093/femsec/fiw096 2016-06-12T00:14:08Z Filamentous green algae of the genus Zygnema (Zygnematophyceae, Streptophyta) are key components of polar hydro-terrestrial mats where they face various stressors including UV irradiation, freezing, desiccation and osmotic stress. Their vegetative cells can develop into pre-akinetes, i.e. reserve-rich, mature cells. We investigated lipid accumulation and fatty acid (FA) composition upon pre-akinete formation in an Arctic and an Antarctic Zygnema strain using transmission electron microscopy and gas chromatography coupled with mass spectrometry. Pre-akinetes formed after 9 weeks of cultivation in nitrogen-free medium, which was accompanied by massive accumulation of lipid bodies. The composition of FAs was similar in both strains, and α-linolenic acid (C18:3) dominated in young vegetative cells. Pre-akinete formation coincided with a significant change in FA composition. Oleic (C18:1) and linoleic (C18:2) acid increased the most (up to 17- and 8-fold, respectively). Small amounts of long-chain polyunsaturated FAs were also detected, e.g. arachidonic (C20:4) and eicosapentaenoic (C20:5) acid. Pre-akinetes exposed to desiccation at 86% relative humidity were able to recover maximum quantum yield of photosystem II, but desiccation had no major effect on FA composition. The results are discussed with regard to the capability of Zygnema spp. to thrive in extreme conditions. Text Antarc* Antarctic Arctic PubMed Central (PMC) Antarctic Arctic FEMS Microbiology Ecology 92 7 fiw096 |
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Research Article Pichrtová, Martina Arc, Erwann Stöggl, Wolfgang Kranner, Ilse Hájek, Tomáš Hackl, Hubert Holzinger, Andreas Formation of lipid bodies and changes in fatty acid composition upon pre-akinete formation in Arctic and Antarctic Zygnema (Zygnematophyceae, Streptophyta) strains |
topic_facet |
Research Article |
description |
Filamentous green algae of the genus Zygnema (Zygnematophyceae, Streptophyta) are key components of polar hydro-terrestrial mats where they face various stressors including UV irradiation, freezing, desiccation and osmotic stress. Their vegetative cells can develop into pre-akinetes, i.e. reserve-rich, mature cells. We investigated lipid accumulation and fatty acid (FA) composition upon pre-akinete formation in an Arctic and an Antarctic Zygnema strain using transmission electron microscopy and gas chromatography coupled with mass spectrometry. Pre-akinetes formed after 9 weeks of cultivation in nitrogen-free medium, which was accompanied by massive accumulation of lipid bodies. The composition of FAs was similar in both strains, and α-linolenic acid (C18:3) dominated in young vegetative cells. Pre-akinete formation coincided with a significant change in FA composition. Oleic (C18:1) and linoleic (C18:2) acid increased the most (up to 17- and 8-fold, respectively). Small amounts of long-chain polyunsaturated FAs were also detected, e.g. arachidonic (C20:4) and eicosapentaenoic (C20:5) acid. Pre-akinetes exposed to desiccation at 86% relative humidity were able to recover maximum quantum yield of photosystem II, but desiccation had no major effect on FA composition. The results are discussed with regard to the capability of Zygnema spp. to thrive in extreme conditions. |
format |
Text |
author |
Pichrtová, Martina Arc, Erwann Stöggl, Wolfgang Kranner, Ilse Hájek, Tomáš Hackl, Hubert Holzinger, Andreas |
author_facet |
Pichrtová, Martina Arc, Erwann Stöggl, Wolfgang Kranner, Ilse Hájek, Tomáš Hackl, Hubert Holzinger, Andreas |
author_sort |
Pichrtová, Martina |
title |
Formation of lipid bodies and changes in fatty acid composition upon pre-akinete formation in Arctic and Antarctic Zygnema (Zygnematophyceae, Streptophyta) strains |
title_short |
Formation of lipid bodies and changes in fatty acid composition upon pre-akinete formation in Arctic and Antarctic Zygnema (Zygnematophyceae, Streptophyta) strains |
title_full |
Formation of lipid bodies and changes in fatty acid composition upon pre-akinete formation in Arctic and Antarctic Zygnema (Zygnematophyceae, Streptophyta) strains |
title_fullStr |
Formation of lipid bodies and changes in fatty acid composition upon pre-akinete formation in Arctic and Antarctic Zygnema (Zygnematophyceae, Streptophyta) strains |
title_full_unstemmed |
Formation of lipid bodies and changes in fatty acid composition upon pre-akinete formation in Arctic and Antarctic Zygnema (Zygnematophyceae, Streptophyta) strains |
title_sort |
formation of lipid bodies and changes in fatty acid composition upon pre-akinete formation in arctic and antarctic zygnema (zygnematophyceae, streptophyta) strains |
publisher |
Oxford University Press |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892695/ http://www.ncbi.nlm.nih.gov/pubmed/27170362 https://doi.org/10.1093/femsec/fiw096 |
geographic |
Antarctic Arctic |
geographic_facet |
Antarctic Arctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892695/ http://www.ncbi.nlm.nih.gov/pubmed/27170362 http://dx.doi.org/10.1093/femsec/fiw096 |
op_rights |
© FEMS 2016. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1093/femsec/fiw096 |
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FEMS Microbiology Ecology |
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92 |
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7 |
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fiw096 |
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1766271232420872192 |