First Isolates of Leptospira spp., from Rodents Captured in Angola
Rodents play an important role in the transmission of pathogenic Leptospira spp. However, in Angola, neither the natural reservoirs of these spirochetes nor leptospirosis diagnosis has been considered. Regarding this gap, we captured rodents in Luanda and Huambo provinces to identify circulating Lep...
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ftpubmed:oai:pubmedcentral.nih.gov:4856626 2023-05-15T18:05:13+02:00 First Isolates of Leptospira spp., from Rodents Captured in Angola Fortes-Gabriel, Elsa Carreira, Teresa Vieira, Maria Luísa 2016-05-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856626/ http://www.ncbi.nlm.nih.gov/pubmed/26928840 https://doi.org/10.4269/ajtmh.15-0027 en eng The American Society of Tropical Medicine and Hygiene http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856626/ http://www.ncbi.nlm.nih.gov/pubmed/26928840 http://dx.doi.org/10.4269/ajtmh.15-0027 ©The American Society of Tropical Medicine and Hygiene This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Articles Text 2016 ftpubmed https://doi.org/10.4269/ajtmh.15-0027 2016-05-22T00:09:29Z Rodents play an important role in the transmission of pathogenic Leptospira spp. However, in Angola, neither the natural reservoirs of these spirochetes nor leptospirosis diagnosis has been considered. Regarding this gap, we captured rodents in Luanda and Huambo provinces to identify circulating Leptospira spp. Rodent kidney tissue was cultured and DNA amplified and sequenced. Culture isolates were evaluated for pathogenic status and typing with rabbit antisera; polymerase chain reaction (PCR) and sequencing were also performed. A total of 37 rodents were captured: Rattus rattus (15, 40.5%), Rattus norvegicus (9, 24.3%), and Mus musculus (13, 35.2%). Leptospiral DNA was amplified in eight (21.6%) kidney samples. From the cultures, we obtained four (10.8%) Leptospira isolates belonging to the Icterohaemorrhagiae and Ballum serogroups of Leptospira interrogans and Leptospira borgpetersenii genospecies, respectively. This study provides information about circulating leptospires spread by rats and mice in Angola. Text Rattus rattus PubMed Central (PMC) The American Journal of Tropical Medicine and Hygiene 94 5 955 958 |
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Articles Fortes-Gabriel, Elsa Carreira, Teresa Vieira, Maria Luísa First Isolates of Leptospira spp., from Rodents Captured in Angola |
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description |
Rodents play an important role in the transmission of pathogenic Leptospira spp. However, in Angola, neither the natural reservoirs of these spirochetes nor leptospirosis diagnosis has been considered. Regarding this gap, we captured rodents in Luanda and Huambo provinces to identify circulating Leptospira spp. Rodent kidney tissue was cultured and DNA amplified and sequenced. Culture isolates were evaluated for pathogenic status and typing with rabbit antisera; polymerase chain reaction (PCR) and sequencing were also performed. A total of 37 rodents were captured: Rattus rattus (15, 40.5%), Rattus norvegicus (9, 24.3%), and Mus musculus (13, 35.2%). Leptospiral DNA was amplified in eight (21.6%) kidney samples. From the cultures, we obtained four (10.8%) Leptospira isolates belonging to the Icterohaemorrhagiae and Ballum serogroups of Leptospira interrogans and Leptospira borgpetersenii genospecies, respectively. This study provides information about circulating leptospires spread by rats and mice in Angola. |
format |
Text |
author |
Fortes-Gabriel, Elsa Carreira, Teresa Vieira, Maria Luísa |
author_facet |
Fortes-Gabriel, Elsa Carreira, Teresa Vieira, Maria Luísa |
author_sort |
Fortes-Gabriel, Elsa |
title |
First Isolates of Leptospira spp., from Rodents Captured in Angola |
title_short |
First Isolates of Leptospira spp., from Rodents Captured in Angola |
title_full |
First Isolates of Leptospira spp., from Rodents Captured in Angola |
title_fullStr |
First Isolates of Leptospira spp., from Rodents Captured in Angola |
title_full_unstemmed |
First Isolates of Leptospira spp., from Rodents Captured in Angola |
title_sort |
first isolates of leptospira spp., from rodents captured in angola |
publisher |
The American Society of Tropical Medicine and Hygiene |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856626/ http://www.ncbi.nlm.nih.gov/pubmed/26928840 https://doi.org/10.4269/ajtmh.15-0027 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856626/ http://www.ncbi.nlm.nih.gov/pubmed/26928840 http://dx.doi.org/10.4269/ajtmh.15-0027 |
op_rights |
©The American Society of Tropical Medicine and Hygiene This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.4269/ajtmh.15-0027 |
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The American Journal of Tropical Medicine and Hygiene |
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94 |
container_issue |
5 |
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955 |
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958 |
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1766176676617650176 |