The miRNA biogenesis in marine bivalves
Small non-coding RNAs include powerful regulators of gene expression, transposon mobility and virus activity. Among the various categories, mature microRNAs (miRNAs) guide the translational repression and decay of several targeted mRNAs. The biogenesis of miRNAs depends on few gene products, essenti...
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ftpubmed:oai:pubmedcentral.nih.gov:4793324 2023-05-15T15:58:38+02:00 The miRNA biogenesis in marine bivalves Rosani, Umberto Pallavicini, Alberto Venier, Paola 2016-03-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793324/ https://doi.org/10.7717/peerj.1763 en eng PeerJ Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793324/ http://dx.doi.org/10.7717/peerj.1763 ©2016 Rosani et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. CC-BY Aquaculture Fisheries and Fish Science Text 2016 ftpubmed https://doi.org/10.7717/peerj.1763 2016-03-20T01:40:23Z Small non-coding RNAs include powerful regulators of gene expression, transposon mobility and virus activity. Among the various categories, mature microRNAs (miRNAs) guide the translational repression and decay of several targeted mRNAs. The biogenesis of miRNAs depends on few gene products, essentially conserved from basal to higher metazoans, whose protein domains allow specific interactions with dsRNA. Here, we report the identification of key genes responsible of the miRNA biogenesis in 32 bivalves, with particular attention to the aquaculture species Mytilus galloprovincialis and Crassostrea gigas. In detail, we have identified and phylogenetically compared eight evolutionary conserved proteins: DROSHA, DGCR8, EXP5, RAN, DICER TARBP2, AGO and PIWI. In mussels, we recognized several other proteins participating in the miRNA biogenesis or in the subsequent RNA silencing. According to digital expression analysis, these genes display low and not inducible expression levels in adult mussels and oysters whereas they are considerably expressed during development. As miRNAs play an important role also in the antiviral responses, knowledge on their production and regulative effects can shed light on essential molecular processes and provide new hints for disease prevention in bivalves. Text Crassostrea gigas PubMed Central (PMC) PeerJ 4 e1763 |
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Aquaculture Fisheries and Fish Science |
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Aquaculture Fisheries and Fish Science Rosani, Umberto Pallavicini, Alberto Venier, Paola The miRNA biogenesis in marine bivalves |
topic_facet |
Aquaculture Fisheries and Fish Science |
description |
Small non-coding RNAs include powerful regulators of gene expression, transposon mobility and virus activity. Among the various categories, mature microRNAs (miRNAs) guide the translational repression and decay of several targeted mRNAs. The biogenesis of miRNAs depends on few gene products, essentially conserved from basal to higher metazoans, whose protein domains allow specific interactions with dsRNA. Here, we report the identification of key genes responsible of the miRNA biogenesis in 32 bivalves, with particular attention to the aquaculture species Mytilus galloprovincialis and Crassostrea gigas. In detail, we have identified and phylogenetically compared eight evolutionary conserved proteins: DROSHA, DGCR8, EXP5, RAN, DICER TARBP2, AGO and PIWI. In mussels, we recognized several other proteins participating in the miRNA biogenesis or in the subsequent RNA silencing. According to digital expression analysis, these genes display low and not inducible expression levels in adult mussels and oysters whereas they are considerably expressed during development. As miRNAs play an important role also in the antiviral responses, knowledge on their production and regulative effects can shed light on essential molecular processes and provide new hints for disease prevention in bivalves. |
format |
Text |
author |
Rosani, Umberto Pallavicini, Alberto Venier, Paola |
author_facet |
Rosani, Umberto Pallavicini, Alberto Venier, Paola |
author_sort |
Rosani, Umberto |
title |
The miRNA biogenesis in marine bivalves |
title_short |
The miRNA biogenesis in marine bivalves |
title_full |
The miRNA biogenesis in marine bivalves |
title_fullStr |
The miRNA biogenesis in marine bivalves |
title_full_unstemmed |
The miRNA biogenesis in marine bivalves |
title_sort |
mirna biogenesis in marine bivalves |
publisher |
PeerJ Inc. |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793324/ https://doi.org/10.7717/peerj.1763 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793324/ http://dx.doi.org/10.7717/peerj.1763 |
op_rights |
©2016 Rosani et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
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CC-BY |
op_doi |
https://doi.org/10.7717/peerj.1763 |
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PeerJ |
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4 |
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e1763 |
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1766394411010228224 |