Cloning HSP70 and HSP90 genes of kaluga (Huso dauricus) and the effects of temperature and salinity stress on their gene expression
The genes encoding HSP70 and HSP90 proteins were isolated from kaluga by homologous cloning and rapid amplification of complementary DNA (cDNA) ends (RACE). HSP70 (GenBank accession no. KP050541) and HSP90 (GenBank accession no. KP050542) cDNAs were composed of 2275 and 2718 bp and encoded polypepti...
Published in: | Cell Stress and Chaperones |
---|---|
Main Authors: | , , , , , , |
Format: | Text |
Language: | English |
Published: |
Springer Netherlands
2015
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786522/ http://www.ncbi.nlm.nih.gov/pubmed/26683614 https://doi.org/10.1007/s12192-015-0665-1 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:4786522 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:4786522 2023-05-15T13:01:54+02:00 Cloning HSP70 and HSP90 genes of kaluga (Huso dauricus) and the effects of temperature and salinity stress on their gene expression Peng, Guogan Zhao, Wen Shi, Zhenguang Chen, Huirong Liu, Yang Wei, Jie Gao, Fengying 2015-12-18 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786522/ http://www.ncbi.nlm.nih.gov/pubmed/26683614 https://doi.org/10.1007/s12192-015-0665-1 en eng Springer Netherlands http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786522/ http://www.ncbi.nlm.nih.gov/pubmed/26683614 http://dx.doi.org/10.1007/s12192-015-0665-1 © Cell Stress Society International 2015 Original Paper Text 2015 ftpubmed https://doi.org/10.1007/s12192-015-0665-1 2016-09-04T00:15:50Z The genes encoding HSP70 and HSP90 proteins were isolated from kaluga by homologous cloning and rapid amplification of complementary DNA (cDNA) ends (RACE). HSP70 (GenBank accession no. KP050541) and HSP90 (GenBank accession no. KP050542) cDNAs were composed of 2275 and 2718 bp and encoded polypeptides of 650 and 725 amino acids, respectively. Basic Local Alignment Search Tool (BLAST) analysis showed that HSP70 and HSP90 of kaluga shared high identities with those of Acipenser ruthenus, Acipenser schrenckii, and Acipenser baerii (98–99 %). Fluorescent real-time RT-PCR under unstressed conditions revealed that HSP70 and HSP90 were expressed in 11 different tissues of kaluga. Messenger RNA (mRNA) expressions of both HSP70 and HSP90 were highest in the intestine and lowest in the muscle. In addition, the patterns of mRNA expression of HSP70 and HSP90 were similar, although the level of expression was more in HSP90 than in HSP70 (P < 0.05).We also analyzed patterns of HSP70 and HSP90 expression in the muscle, gill, and liver of kaluga under different combinations of temperature and salinity stress, including temperatures of 4,10, 25, and 28 °C at 0 ppt salinity, and salinities of 10, 20, 30, and 40 ppt at 16 °C, where 16 °C at 0 ppt (parts per thousand) served as the control. We found that levels of mRNA expression of both HSP70 and HSP90 were highest at 4 °C in the muscle, gill, and liver and changed little with salinity stress. These results increase understanding of the mechanisms of stress response of cold freshwater fish. Text Acipenser baerii PubMed Central (PMC) Cell Stress and Chaperones 21 2 349 359 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Original Paper |
spellingShingle |
Original Paper Peng, Guogan Zhao, Wen Shi, Zhenguang Chen, Huirong Liu, Yang Wei, Jie Gao, Fengying Cloning HSP70 and HSP90 genes of kaluga (Huso dauricus) and the effects of temperature and salinity stress on their gene expression |
topic_facet |
Original Paper |
description |
The genes encoding HSP70 and HSP90 proteins were isolated from kaluga by homologous cloning and rapid amplification of complementary DNA (cDNA) ends (RACE). HSP70 (GenBank accession no. KP050541) and HSP90 (GenBank accession no. KP050542) cDNAs were composed of 2275 and 2718 bp and encoded polypeptides of 650 and 725 amino acids, respectively. Basic Local Alignment Search Tool (BLAST) analysis showed that HSP70 and HSP90 of kaluga shared high identities with those of Acipenser ruthenus, Acipenser schrenckii, and Acipenser baerii (98–99 %). Fluorescent real-time RT-PCR under unstressed conditions revealed that HSP70 and HSP90 were expressed in 11 different tissues of kaluga. Messenger RNA (mRNA) expressions of both HSP70 and HSP90 were highest in the intestine and lowest in the muscle. In addition, the patterns of mRNA expression of HSP70 and HSP90 were similar, although the level of expression was more in HSP90 than in HSP70 (P < 0.05).We also analyzed patterns of HSP70 and HSP90 expression in the muscle, gill, and liver of kaluga under different combinations of temperature and salinity stress, including temperatures of 4,10, 25, and 28 °C at 0 ppt salinity, and salinities of 10, 20, 30, and 40 ppt at 16 °C, where 16 °C at 0 ppt (parts per thousand) served as the control. We found that levels of mRNA expression of both HSP70 and HSP90 were highest at 4 °C in the muscle, gill, and liver and changed little with salinity stress. These results increase understanding of the mechanisms of stress response of cold freshwater fish. |
format |
Text |
author |
Peng, Guogan Zhao, Wen Shi, Zhenguang Chen, Huirong Liu, Yang Wei, Jie Gao, Fengying |
author_facet |
Peng, Guogan Zhao, Wen Shi, Zhenguang Chen, Huirong Liu, Yang Wei, Jie Gao, Fengying |
author_sort |
Peng, Guogan |
title |
Cloning HSP70 and HSP90 genes of kaluga (Huso dauricus) and the effects of temperature and salinity stress on their gene expression |
title_short |
Cloning HSP70 and HSP90 genes of kaluga (Huso dauricus) and the effects of temperature and salinity stress on their gene expression |
title_full |
Cloning HSP70 and HSP90 genes of kaluga (Huso dauricus) and the effects of temperature and salinity stress on their gene expression |
title_fullStr |
Cloning HSP70 and HSP90 genes of kaluga (Huso dauricus) and the effects of temperature and salinity stress on their gene expression |
title_full_unstemmed |
Cloning HSP70 and HSP90 genes of kaluga (Huso dauricus) and the effects of temperature and salinity stress on their gene expression |
title_sort |
cloning hsp70 and hsp90 genes of kaluga (huso dauricus) and the effects of temperature and salinity stress on their gene expression |
publisher |
Springer Netherlands |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786522/ http://www.ncbi.nlm.nih.gov/pubmed/26683614 https://doi.org/10.1007/s12192-015-0665-1 |
genre |
Acipenser baerii |
genre_facet |
Acipenser baerii |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786522/ http://www.ncbi.nlm.nih.gov/pubmed/26683614 http://dx.doi.org/10.1007/s12192-015-0665-1 |
op_rights |
© Cell Stress Society International 2015 |
op_doi |
https://doi.org/10.1007/s12192-015-0665-1 |
container_title |
Cell Stress and Chaperones |
container_volume |
21 |
container_issue |
2 |
container_start_page |
349 |
op_container_end_page |
359 |
_version_ |
1766289707016126464 |