Light Levels Affect Carbon Utilisation in Tropical Seagrass under Ocean Acidification
Under future ocean acidification (OA), increased availability of dissolved inorganic carbon (DIC) in seawater may enhance seagrass productivity. However, the ability to utilise additional DIC could be regulated by light availability, often reduced through land runoff. To test this, two tropical seag...
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ftpubmed:oai:pubmedcentral.nih.gov:4777487 2023-05-15T17:50:45+02:00 Light Levels Affect Carbon Utilisation in Tropical Seagrass under Ocean Acidification Ow, Yan X. Uthicke, Sven Collier, Catherine J. 2016-03-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777487/ http://www.ncbi.nlm.nih.gov/pubmed/26938454 https://doi.org/10.1371/journal.pone.0150352 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777487/ http://www.ncbi.nlm.nih.gov/pubmed/26938454 http://dx.doi.org/10.1371/journal.pone.0150352 © 2016 Ow et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2016 ftpubmed https://doi.org/10.1371/journal.pone.0150352 2016-03-20T01:25:47Z Under future ocean acidification (OA), increased availability of dissolved inorganic carbon (DIC) in seawater may enhance seagrass productivity. However, the ability to utilise additional DIC could be regulated by light availability, often reduced through land runoff. To test this, two tropical seagrass species, Cymodocea serrulata and Halodule uninervis were exposed to two DIC concentrations (447 μatm and 1077 μatm pCO2), and three light treatments (35, 100, 380 μmol m-2 s-1) for two weeks. DIC uptake mechanisms were separately examined by measuring net photosynthetic rates while subjecting C. serrulata and H. uninervis to changes in light and addition of bicarbonate (HCO3-) use inhibitors (carbonic anhydrase inhibitor, acetazolamide) and TRIS buffer (pH 8.0). We observed a strong dependence on energy driven H+-HCO3- co-transport (TRIS, which disrupts H+ extrusion) in C. serrulata under all light levels, indicating greater CO2 dependence in low light. This was confirmed when, after two weeks exposure, DIC enrichment stimulated maximum photosynthetic rates (Pmax) and efficiency (α) more in C. serrulata grown under lower light levels (36–60% increase) than for those in high light (4% increase). However, C. serrulata growth increased with both DIC enrichment and light levels. Growth, NPP and photosynthetic responses in H. uninervis increased with higher light treatments and were independent of DIC availability. Furthermore, H. uninervis was found to be more flexible in HCO3- uptake pathways. Here, light availability influenced productivity responses to DIC enrichment, via both carbon fixation and acquisition processes, highlighting the role of water quality in future responses to OA. Text Ocean acidification PubMed Central (PMC) PLOS ONE 11 3 e0150352 |
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Research Article Ow, Yan X. Uthicke, Sven Collier, Catherine J. Light Levels Affect Carbon Utilisation in Tropical Seagrass under Ocean Acidification |
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Research Article |
description |
Under future ocean acidification (OA), increased availability of dissolved inorganic carbon (DIC) in seawater may enhance seagrass productivity. However, the ability to utilise additional DIC could be regulated by light availability, often reduced through land runoff. To test this, two tropical seagrass species, Cymodocea serrulata and Halodule uninervis were exposed to two DIC concentrations (447 μatm and 1077 μatm pCO2), and three light treatments (35, 100, 380 μmol m-2 s-1) for two weeks. DIC uptake mechanisms were separately examined by measuring net photosynthetic rates while subjecting C. serrulata and H. uninervis to changes in light and addition of bicarbonate (HCO3-) use inhibitors (carbonic anhydrase inhibitor, acetazolamide) and TRIS buffer (pH 8.0). We observed a strong dependence on energy driven H+-HCO3- co-transport (TRIS, which disrupts H+ extrusion) in C. serrulata under all light levels, indicating greater CO2 dependence in low light. This was confirmed when, after two weeks exposure, DIC enrichment stimulated maximum photosynthetic rates (Pmax) and efficiency (α) more in C. serrulata grown under lower light levels (36–60% increase) than for those in high light (4% increase). However, C. serrulata growth increased with both DIC enrichment and light levels. Growth, NPP and photosynthetic responses in H. uninervis increased with higher light treatments and were independent of DIC availability. Furthermore, H. uninervis was found to be more flexible in HCO3- uptake pathways. Here, light availability influenced productivity responses to DIC enrichment, via both carbon fixation and acquisition processes, highlighting the role of water quality in future responses to OA. |
format |
Text |
author |
Ow, Yan X. Uthicke, Sven Collier, Catherine J. |
author_facet |
Ow, Yan X. Uthicke, Sven Collier, Catherine J. |
author_sort |
Ow, Yan X. |
title |
Light Levels Affect Carbon Utilisation in Tropical Seagrass under Ocean Acidification |
title_short |
Light Levels Affect Carbon Utilisation in Tropical Seagrass under Ocean Acidification |
title_full |
Light Levels Affect Carbon Utilisation in Tropical Seagrass under Ocean Acidification |
title_fullStr |
Light Levels Affect Carbon Utilisation in Tropical Seagrass under Ocean Acidification |
title_full_unstemmed |
Light Levels Affect Carbon Utilisation in Tropical Seagrass under Ocean Acidification |
title_sort |
light levels affect carbon utilisation in tropical seagrass under ocean acidification |
publisher |
Public Library of Science |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777487/ http://www.ncbi.nlm.nih.gov/pubmed/26938454 https://doi.org/10.1371/journal.pone.0150352 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777487/ http://www.ncbi.nlm.nih.gov/pubmed/26938454 http://dx.doi.org/10.1371/journal.pone.0150352 |
op_rights |
© 2016 Ow et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
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https://doi.org/10.1371/journal.pone.0150352 |
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PLOS ONE |
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