Assessment of protein synthesis in highly aerobic canine species at the onset and during exercise training
Canis lupus familiaris, the domesticated dog, is capable of extreme endurance performance. The ability to perform sustained aerobic exercise is dependent on a well-developed mitochondrial reticulum. In this study we examined the cumulative muscle protein and DNA synthesis in groups of athletic dogs...
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ftpubmed:oai:pubmedcentral.nih.gov:4747891 2023-05-15T15:50:58+02:00 Assessment of protein synthesis in highly aerobic canine species at the onset and during exercise training Miller, Benjamin F. Ehrlicher, Sarah E. Drake, Joshua C. Peelor, Frederick F. Biela, Laurie M. Pratt-Phillips, Shannon Davis, Michael Hamilton, Karyn L. 2015-01-22 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747891/ http://www.ncbi.nlm.nih.gov/pubmed/25614602 https://doi.org/10.1152/japplphysiol.00982.2014 en eng American Physiological Society http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747891/ http://www.ncbi.nlm.nih.gov/pubmed/25614602 http://dx.doi.org/10.1152/japplphysiol.00982.2014 Copyright © 2015 the American Physiological Society Articles Text 2015 ftpubmed https://doi.org/10.1152/japplphysiol.00982.2014 2016-04-03T00:16:18Z Canis lupus familiaris, the domesticated dog, is capable of extreme endurance performance. The ability to perform sustained aerobic exercise is dependent on a well-developed mitochondrial reticulum. In this study we examined the cumulative muscle protein and DNA synthesis in groups of athletic dogs at the onset of an exercise training program and following a strenuous exercise training program. We hypothesized that both at the onset and during an exercise training program there would be greater mitochondrial protein synthesis rates compared with sedentary control with no difference in mixed or cytoplasmic protein synthesis rates. Protein synthetic rates of three protein fractions and DNA synthesis were determined over 1 wk using 2H2O in competitive Alaskan Huskies and Labrador Retrievers trained for explosive device detection. Both groups of dogs had very high rates of skeletal muscle protein synthesis in the sedentary state [Alaskan Huskies: Mixed = 2.28 ± 0.12, cytoplasmic (Cyto) = 2.91 ± 0.10, and mitochondrial (Mito) = 2.62 ± 0.07; Labrador Retrievers: Mixed = 3.88 ± 0.37, Cyto = 3.85 ± 0.06, and Mito = 2.92 ± 0.20%/day]. Mitochondrial (Mito) protein synthesis rates did not increase at the onset of an exercise training program. Exercise-trained dogs maintained Mito protein synthesis during exercise training when mixed (Mixed) and cytosolic (Cyto) fractions decreased, and this coincided with a decrease in p-RpS6 but also a decrease in p-ACC signaling. Contrary to our hypothesis, canines did not have large increases in mitochondrial protein synthesis at the onset or during an exercise training program. However, dogs have a high rate of protein synthesis compared with humans that perhaps does not necessitate an extra increase in protein synthesis at the onset of aerobic exercise training. Text Canis lupus Huskies PubMed Central (PMC) Journal of Applied Physiology 118 7 811 817 |
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Articles Miller, Benjamin F. Ehrlicher, Sarah E. Drake, Joshua C. Peelor, Frederick F. Biela, Laurie M. Pratt-Phillips, Shannon Davis, Michael Hamilton, Karyn L. Assessment of protein synthesis in highly aerobic canine species at the onset and during exercise training |
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Canis lupus familiaris, the domesticated dog, is capable of extreme endurance performance. The ability to perform sustained aerobic exercise is dependent on a well-developed mitochondrial reticulum. In this study we examined the cumulative muscle protein and DNA synthesis in groups of athletic dogs at the onset of an exercise training program and following a strenuous exercise training program. We hypothesized that both at the onset and during an exercise training program there would be greater mitochondrial protein synthesis rates compared with sedentary control with no difference in mixed or cytoplasmic protein synthesis rates. Protein synthetic rates of three protein fractions and DNA synthesis were determined over 1 wk using 2H2O in competitive Alaskan Huskies and Labrador Retrievers trained for explosive device detection. Both groups of dogs had very high rates of skeletal muscle protein synthesis in the sedentary state [Alaskan Huskies: Mixed = 2.28 ± 0.12, cytoplasmic (Cyto) = 2.91 ± 0.10, and mitochondrial (Mito) = 2.62 ± 0.07; Labrador Retrievers: Mixed = 3.88 ± 0.37, Cyto = 3.85 ± 0.06, and Mito = 2.92 ± 0.20%/day]. Mitochondrial (Mito) protein synthesis rates did not increase at the onset of an exercise training program. Exercise-trained dogs maintained Mito protein synthesis during exercise training when mixed (Mixed) and cytosolic (Cyto) fractions decreased, and this coincided with a decrease in p-RpS6 but also a decrease in p-ACC signaling. Contrary to our hypothesis, canines did not have large increases in mitochondrial protein synthesis at the onset or during an exercise training program. However, dogs have a high rate of protein synthesis compared with humans that perhaps does not necessitate an extra increase in protein synthesis at the onset of aerobic exercise training. |
format |
Text |
author |
Miller, Benjamin F. Ehrlicher, Sarah E. Drake, Joshua C. Peelor, Frederick F. Biela, Laurie M. Pratt-Phillips, Shannon Davis, Michael Hamilton, Karyn L. |
author_facet |
Miller, Benjamin F. Ehrlicher, Sarah E. Drake, Joshua C. Peelor, Frederick F. Biela, Laurie M. Pratt-Phillips, Shannon Davis, Michael Hamilton, Karyn L. |
author_sort |
Miller, Benjamin F. |
title |
Assessment of protein synthesis in highly aerobic canine species at the onset and during exercise training |
title_short |
Assessment of protein synthesis in highly aerobic canine species at the onset and during exercise training |
title_full |
Assessment of protein synthesis in highly aerobic canine species at the onset and during exercise training |
title_fullStr |
Assessment of protein synthesis in highly aerobic canine species at the onset and during exercise training |
title_full_unstemmed |
Assessment of protein synthesis in highly aerobic canine species at the onset and during exercise training |
title_sort |
assessment of protein synthesis in highly aerobic canine species at the onset and during exercise training |
publisher |
American Physiological Society |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747891/ http://www.ncbi.nlm.nih.gov/pubmed/25614602 https://doi.org/10.1152/japplphysiol.00982.2014 |
genre |
Canis lupus Huskies |
genre_facet |
Canis lupus Huskies |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747891/ http://www.ncbi.nlm.nih.gov/pubmed/25614602 http://dx.doi.org/10.1152/japplphysiol.00982.2014 |
op_rights |
Copyright © 2015 the American Physiological Society |
op_doi |
https://doi.org/10.1152/japplphysiol.00982.2014 |
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Journal of Applied Physiology |
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118 |
container_issue |
7 |
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811 |
op_container_end_page |
817 |
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1766386012864380928 |