Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy
Diatoms, an important group of phytoplankton, bloom annually in the Southern Ocean, covering thousands of square kilometers and dominating the region's phytoplankton communities. In their role as the major food source to marine grazers, diatoms supply carbon, nutrients and energy to the Souther...
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ftpubmed:oai:pubmedcentral.nih.gov:4737933 2023-05-15T18:24:35+02:00 Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy Sackett, Olivia Petrou, Katherina Reedy, Brian Hill, Ross Doblin, Martina Beardall, John Ralph, Peter Heraud, Philip 2016-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737933/ http://www.ncbi.nlm.nih.gov/pubmed/26230047 https://doi.org/10.1038/ismej.2015.123 en eng Nature Publishing Group http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737933/ http://www.ncbi.nlm.nih.gov/pubmed/26230047 http://dx.doi.org/10.1038/ismej.2015.123 Copyright © 2016 International Society for Microbial Ecology Original Article Text 2016 ftpubmed https://doi.org/10.1038/ismej.2015.123 2017-02-05T01:03:14Z Diatoms, an important group of phytoplankton, bloom annually in the Southern Ocean, covering thousands of square kilometers and dominating the region's phytoplankton communities. In their role as the major food source to marine grazers, diatoms supply carbon, nutrients and energy to the Southern Ocean food web. Prevailing environmental conditions influence diatom phenotypic traits (for example, photophysiology, macromolecular composition and morphology), which in turn affect the transfer of energy, carbon and nutrients to grazers and higher trophic levels, as well as oceanic biogeochemical cycles. The paucity of phenotypic data on Southern Ocean phytoplankton limits our understanding of the ecosystem and how it may respond to future environmental change. Here we used a novel approach to create a ‘snapshot' of cell phenotype. Using mass spectrometry, we measured nitrogen (a proxy for protein), total carbon and carbon-13 enrichment (carbon productivity), then used this data to build spectroscopy-based predictive models. The models were used to provide phenotypic data for samples from a third sample set. Importantly, this approach enabled the first ever rate determination of carbon productivity from a single time point, circumventing the need for time-series measurements. This study showed that Chaetoceros simplex was less productive and had lower protein and carbon content during short-term periods of high salinity. Applying this new phenomics approach to natural phytoplankton samples could provide valuable insight into understanding phytoplankton productivity and function in the marine system. Text Southern Ocean PubMed Central (PMC) Southern Ocean The ISME Journal 10 2 416 426 |
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Original Article Sackett, Olivia Petrou, Katherina Reedy, Brian Hill, Ross Doblin, Martina Beardall, John Ralph, Peter Heraud, Philip Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy |
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Original Article |
description |
Diatoms, an important group of phytoplankton, bloom annually in the Southern Ocean, covering thousands of square kilometers and dominating the region's phytoplankton communities. In their role as the major food source to marine grazers, diatoms supply carbon, nutrients and energy to the Southern Ocean food web. Prevailing environmental conditions influence diatom phenotypic traits (for example, photophysiology, macromolecular composition and morphology), which in turn affect the transfer of energy, carbon and nutrients to grazers and higher trophic levels, as well as oceanic biogeochemical cycles. The paucity of phenotypic data on Southern Ocean phytoplankton limits our understanding of the ecosystem and how it may respond to future environmental change. Here we used a novel approach to create a ‘snapshot' of cell phenotype. Using mass spectrometry, we measured nitrogen (a proxy for protein), total carbon and carbon-13 enrichment (carbon productivity), then used this data to build spectroscopy-based predictive models. The models were used to provide phenotypic data for samples from a third sample set. Importantly, this approach enabled the first ever rate determination of carbon productivity from a single time point, circumventing the need for time-series measurements. This study showed that Chaetoceros simplex was less productive and had lower protein and carbon content during short-term periods of high salinity. Applying this new phenomics approach to natural phytoplankton samples could provide valuable insight into understanding phytoplankton productivity and function in the marine system. |
format |
Text |
author |
Sackett, Olivia Petrou, Katherina Reedy, Brian Hill, Ross Doblin, Martina Beardall, John Ralph, Peter Heraud, Philip |
author_facet |
Sackett, Olivia Petrou, Katherina Reedy, Brian Hill, Ross Doblin, Martina Beardall, John Ralph, Peter Heraud, Philip |
author_sort |
Sackett, Olivia |
title |
Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy |
title_short |
Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy |
title_full |
Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy |
title_fullStr |
Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy |
title_full_unstemmed |
Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy |
title_sort |
snapshot prediction of carbon productivity, carbon and protein content in a southern ocean diatom using ftir spectroscopy |
publisher |
Nature Publishing Group |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737933/ http://www.ncbi.nlm.nih.gov/pubmed/26230047 https://doi.org/10.1038/ismej.2015.123 |
geographic |
Southern Ocean |
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Southern Ocean |
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Southern Ocean |
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Southern Ocean |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737933/ http://www.ncbi.nlm.nih.gov/pubmed/26230047 http://dx.doi.org/10.1038/ismej.2015.123 |
op_rights |
Copyright © 2016 International Society for Microbial Ecology |
op_doi |
https://doi.org/10.1038/ismej.2015.123 |
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The ISME Journal |
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10 |
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2 |
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416 |
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426 |
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1766205266615861248 |