Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs
Oil reservoirs are major sites of methane production and carbon turnover, processes with significant impacts on energy resources and global biogeochemical cycles. We applied a cultivation-independent genomic approach to define microbial community membership and predict roles for specific organisms i...
Published in: | mBio |
---|---|
Main Authors: | , , , , , , , |
Format: | Text |
Language: | English |
Published: |
American Society of Microbiology
2016
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725000/ http://www.ncbi.nlm.nih.gov/pubmed/26787827 https://doi.org/10.1128/mBio.01669-15 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:4725000 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:4725000 2023-05-15T13:09:10+02:00 Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs Hu, Ping Tom, Lauren Singh, Andrea Thomas, Brian C. Baker, Brett J. Piceno, Yvette M. Andersen, Gary L. Banfield, Jillian F. 2016-01-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725000/ http://www.ncbi.nlm.nih.gov/pubmed/26787827 https://doi.org/10.1128/mBio.01669-15 en eng American Society of Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725000/ http://www.ncbi.nlm.nih.gov/pubmed/26787827 http://dx.doi.org/10.1128/mBio.01669-15 Copyright © 2016 Hu et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY-NC-SA Research Article Text 2016 ftpubmed https://doi.org/10.1128/mBio.01669-15 2016-01-31T01:21:19Z Oil reservoirs are major sites of methane production and carbon turnover, processes with significant impacts on energy resources and global biogeochemical cycles. We applied a cultivation-independent genomic approach to define microbial community membership and predict roles for specific organisms in biogeochemical transformations in Alaska North Slope oil fields. Produced water samples were collected from six locations between 1,128 m (24 to 27°C) and 2,743 m (80 to 83°C) below the surface. Microbial community complexity decreased with increasing temperature, and the potential to degrade hydrocarbon compounds was most prevalent in the lower-temperature reservoirs. Sulfate availability, rather than sulfate reduction potential, seems to be the limiting factor for sulfide production in some of the reservoirs under investigation. Most microorganisms in the intermediate- and higher-temperature samples were related to previously studied methanogenic and nonmethanogenic archaea and thermophilic bacteria, but one candidate phylum bacterium, a member of the Acetothermia (OP1), was present in Kuparuk sample K3. The greatest numbers of candidate phyla were recovered from the mesothermic reservoir samples SB1 and SB2. We reconstructed a nearly complete genome for an organism from the candidate phylum Parcubacteria (OD1) that was abundant in sample SB1. Consistent with prior findings for members of this lineage, the OD1 genome is small, and metabolic predictions support an obligately anaerobic, fermentation-based lifestyle. At moderate abundance in samples SB1 and SB2 were members of bacteria from other candidate phyla, including Microgenomates (OP11), Atribacteria (OP9), candidate phyla TA06 and WS6, and Marinimicrobia (SAR406). The results presented here elucidate potential roles of organisms in oil reservoir biological processes. Text Alaska North Slope north slope Alaska PubMed Central (PMC) mBio 7 1 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Hu, Ping Tom, Lauren Singh, Andrea Thomas, Brian C. Baker, Brett J. Piceno, Yvette M. Andersen, Gary L. Banfield, Jillian F. Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs |
topic_facet |
Research Article |
description |
Oil reservoirs are major sites of methane production and carbon turnover, processes with significant impacts on energy resources and global biogeochemical cycles. We applied a cultivation-independent genomic approach to define microbial community membership and predict roles for specific organisms in biogeochemical transformations in Alaska North Slope oil fields. Produced water samples were collected from six locations between 1,128 m (24 to 27°C) and 2,743 m (80 to 83°C) below the surface. Microbial community complexity decreased with increasing temperature, and the potential to degrade hydrocarbon compounds was most prevalent in the lower-temperature reservoirs. Sulfate availability, rather than sulfate reduction potential, seems to be the limiting factor for sulfide production in some of the reservoirs under investigation. Most microorganisms in the intermediate- and higher-temperature samples were related to previously studied methanogenic and nonmethanogenic archaea and thermophilic bacteria, but one candidate phylum bacterium, a member of the Acetothermia (OP1), was present in Kuparuk sample K3. The greatest numbers of candidate phyla were recovered from the mesothermic reservoir samples SB1 and SB2. We reconstructed a nearly complete genome for an organism from the candidate phylum Parcubacteria (OD1) that was abundant in sample SB1. Consistent with prior findings for members of this lineage, the OD1 genome is small, and metabolic predictions support an obligately anaerobic, fermentation-based lifestyle. At moderate abundance in samples SB1 and SB2 were members of bacteria from other candidate phyla, including Microgenomates (OP11), Atribacteria (OP9), candidate phyla TA06 and WS6, and Marinimicrobia (SAR406). The results presented here elucidate potential roles of organisms in oil reservoir biological processes. |
format |
Text |
author |
Hu, Ping Tom, Lauren Singh, Andrea Thomas, Brian C. Baker, Brett J. Piceno, Yvette M. Andersen, Gary L. Banfield, Jillian F. |
author_facet |
Hu, Ping Tom, Lauren Singh, Andrea Thomas, Brian C. Baker, Brett J. Piceno, Yvette M. Andersen, Gary L. Banfield, Jillian F. |
author_sort |
Hu, Ping |
title |
Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs |
title_short |
Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs |
title_full |
Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs |
title_fullStr |
Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs |
title_full_unstemmed |
Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs |
title_sort |
genome-resolved metagenomic analysis reveals roles for candidate phyla and other microbial community members in biogeochemical transformations in oil reservoirs |
publisher |
American Society of Microbiology |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725000/ http://www.ncbi.nlm.nih.gov/pubmed/26787827 https://doi.org/10.1128/mBio.01669-15 |
genre |
Alaska North Slope north slope Alaska |
genre_facet |
Alaska North Slope north slope Alaska |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725000/ http://www.ncbi.nlm.nih.gov/pubmed/26787827 http://dx.doi.org/10.1128/mBio.01669-15 |
op_rights |
Copyright © 2016 Hu et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY-NC-SA |
op_doi |
https://doi.org/10.1128/mBio.01669-15 |
container_title |
mBio |
container_volume |
7 |
container_issue |
1 |
_version_ |
1766165313552908288 |