Fibrolytic Bacteria Isolated from the Rumen of North American Moose (Alces alces) and Their Use as a Probiotic in Neonatal Lambs
Fibrolytic bacteria were isolated from the rumen of North American moose (Alces alces), which eat a high-fiber diet of woody browse. It was hypothesized that fibrolytic bacteria isolated from the moose rumen could be used as probiotics to improve fiber degradation and animal production. Thirty-one i...
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ftpubmed:oai:pubmedcentral.nih.gov:4696820 2023-05-15T13:13:02+02:00 Fibrolytic Bacteria Isolated from the Rumen of North American Moose (Alces alces) and Their Use as a Probiotic in Neonatal Lambs Ishaq, Suzanne L. Kim, Christina J. Reis, Doug Wright, André-Denis G. 2015-12-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696820/ http://www.ncbi.nlm.nih.gov/pubmed/26716685 https://doi.org/10.1371/journal.pone.0144804 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696820/ http://www.ncbi.nlm.nih.gov/pubmed/26716685 http://dx.doi.org/10.1371/journal.pone.0144804 © 2015 Ishaq et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited CC-BY Research Article Text 2015 ftpubmed https://doi.org/10.1371/journal.pone.0144804 2016-01-17T01:16:47Z Fibrolytic bacteria were isolated from the rumen of North American moose (Alces alces), which eat a high-fiber diet of woody browse. It was hypothesized that fibrolytic bacteria isolated from the moose rumen could be used as probiotics to improve fiber degradation and animal production. Thirty-one isolates (Bacillus, n = 26; Paenibacillus, n = 1; and Staphylococcus, n = 4) were cultured from moose rumen digesta samples collected in Vermont. Using Sanger sequencing of the 16S rRNA gene, culturing techniques, and optical densities, isolates were identified and screened for biochemical properties important to plant carbohydrate degradation. Five isolates were selected as candidates for use as a probiotic, which was administered daily to neonate lambs for 9 weeks. It was hypothesized that regular administration of a probiotic to improve fibrolysis to neonate animals through weaning would increase the developing rumen bacterial diversity, increase animal production, and allow for long-term colonization of the probiotic species. Neither weight gain nor wool quality was improved in lambs given a probiotic, however, dietary efficiency was increased as evidenced by the reduced feed intake (and rearing costs) without a loss to weight gain. Experimental lambs had a lower acetate to propionate ratio than control lambs, which was previously shown to indicate increased dietary efficiency. Fibrolytic bacteria made up the majority of sequences, mainly Prevotella, Butyrivibrio, and Ruminococcus. While protozoal densities increased over time and were stable, methanogen densities varied greatly in the first six months of life for lambs. This is likely due to the changing diet and bacterial populations in the developing rumen. Text Alces alces PubMed Central (PMC) PLOS ONE 10 12 e0144804 |
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Research Article Ishaq, Suzanne L. Kim, Christina J. Reis, Doug Wright, André-Denis G. Fibrolytic Bacteria Isolated from the Rumen of North American Moose (Alces alces) and Their Use as a Probiotic in Neonatal Lambs |
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Research Article |
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Fibrolytic bacteria were isolated from the rumen of North American moose (Alces alces), which eat a high-fiber diet of woody browse. It was hypothesized that fibrolytic bacteria isolated from the moose rumen could be used as probiotics to improve fiber degradation and animal production. Thirty-one isolates (Bacillus, n = 26; Paenibacillus, n = 1; and Staphylococcus, n = 4) were cultured from moose rumen digesta samples collected in Vermont. Using Sanger sequencing of the 16S rRNA gene, culturing techniques, and optical densities, isolates were identified and screened for biochemical properties important to plant carbohydrate degradation. Five isolates were selected as candidates for use as a probiotic, which was administered daily to neonate lambs for 9 weeks. It was hypothesized that regular administration of a probiotic to improve fibrolysis to neonate animals through weaning would increase the developing rumen bacterial diversity, increase animal production, and allow for long-term colonization of the probiotic species. Neither weight gain nor wool quality was improved in lambs given a probiotic, however, dietary efficiency was increased as evidenced by the reduced feed intake (and rearing costs) without a loss to weight gain. Experimental lambs had a lower acetate to propionate ratio than control lambs, which was previously shown to indicate increased dietary efficiency. Fibrolytic bacteria made up the majority of sequences, mainly Prevotella, Butyrivibrio, and Ruminococcus. While protozoal densities increased over time and were stable, methanogen densities varied greatly in the first six months of life for lambs. This is likely due to the changing diet and bacterial populations in the developing rumen. |
format |
Text |
author |
Ishaq, Suzanne L. Kim, Christina J. Reis, Doug Wright, André-Denis G. |
author_facet |
Ishaq, Suzanne L. Kim, Christina J. Reis, Doug Wright, André-Denis G. |
author_sort |
Ishaq, Suzanne L. |
title |
Fibrolytic Bacteria Isolated from the Rumen of North American Moose (Alces alces) and Their Use as a Probiotic in Neonatal Lambs |
title_short |
Fibrolytic Bacteria Isolated from the Rumen of North American Moose (Alces alces) and Their Use as a Probiotic in Neonatal Lambs |
title_full |
Fibrolytic Bacteria Isolated from the Rumen of North American Moose (Alces alces) and Their Use as a Probiotic in Neonatal Lambs |
title_fullStr |
Fibrolytic Bacteria Isolated from the Rumen of North American Moose (Alces alces) and Their Use as a Probiotic in Neonatal Lambs |
title_full_unstemmed |
Fibrolytic Bacteria Isolated from the Rumen of North American Moose (Alces alces) and Their Use as a Probiotic in Neonatal Lambs |
title_sort |
fibrolytic bacteria isolated from the rumen of north american moose (alces alces) and their use as a probiotic in neonatal lambs |
publisher |
Public Library of Science |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696820/ http://www.ncbi.nlm.nih.gov/pubmed/26716685 https://doi.org/10.1371/journal.pone.0144804 |
genre |
Alces alces |
genre_facet |
Alces alces |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696820/ http://www.ncbi.nlm.nih.gov/pubmed/26716685 http://dx.doi.org/10.1371/journal.pone.0144804 |
op_rights |
© 2015 Ishaq et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
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CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0144804 |
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PLOS ONE |
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10 |
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12 |
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e0144804 |
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