Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum
A strain of a hyperthermophilic filamentous archaeon was isolated from a sample of Kamchatka hot spring sediment. Isolate 1807-2 grew optimally at 85 °C, pH 6.0-6.5, the parameters being close to those at the sampling site. 16S rRNA gene sequence analysis placed the novel isolate in the crenarchaeal...
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ftpubmed:oai:pubmedcentral.nih.gov:4673724 2023-05-15T16:59:17+02:00 Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum Toshchakov, Stepan V. Korzhenkov, Aleksei A. Samarov, Nazar I. Mazunin, Ilia O. Mozhey, Oleg I. Shmyr, Ilya S. Derbikova, Ksenia S. Taranov, Evgeny A. Dominova, Irina N. Bonch-Osmolovskaya, Elizaveta A. Patrushev, Maxim V. Podosokorskaya, Olga A. Kublanov, Ilya V. 2015-12-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673724/ https://doi.org/10.1186/s40793-015-0105-y en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673724/ http://dx.doi.org/10.1186/s40793-015-0105-y © Toshchakov et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. CC0 PDM CC-BY Short Genome Report Text 2015 ftpubmed https://doi.org/10.1186/s40793-015-0105-y 2015-12-13T01:27:50Z A strain of a hyperthermophilic filamentous archaeon was isolated from a sample of Kamchatka hot spring sediment. Isolate 1807-2 grew optimally at 85 °C, pH 6.0-6.5, the parameters being close to those at the sampling site. 16S rRNA gene sequence analysis placed the novel isolate in the crenarchaeal genus Thermofilum; Thermofilum pendens was its closest valid relative (95.7 % of sequence identity). Strain 1807-2 grew organothrophically using polysaccharides (starch and glucomannan), yeast extract or peptone as substrates. The addition of other crenarchaea culture broth filtrates was obligatory required for growth and could not be replaced by the addition of these organisms’ cell wall fractions, as it was described for T. pendens. The genome of strain 1807-2 was sequenced using Illumina and PGM technologies. The average nucleotide identities between genome of strain 1807-2 and T. pendens strain HRK 5T and “T. adornatus” strain 1910b were 85 and 82 %, respectively. On the basis of 16S rRNA gene sequence phylogeny, ANI calculations and phenotypic differences we propose a novel species Thermofilum uzonense with the type strain 1807-2T (= DSM 28062T = JCM 19810T). Project information and genome sequence was deposited in Genbank under IDs PRJNA262459 and CP009961, respectively. Text Kamchatka PubMed Central (PMC) Standards in Genomic Sciences 10 1 |
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PubMed Central (PMC) |
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ftpubmed |
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English |
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Short Genome Report |
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Short Genome Report Toshchakov, Stepan V. Korzhenkov, Aleksei A. Samarov, Nazar I. Mazunin, Ilia O. Mozhey, Oleg I. Shmyr, Ilya S. Derbikova, Ksenia S. Taranov, Evgeny A. Dominova, Irina N. Bonch-Osmolovskaya, Elizaveta A. Patrushev, Maxim V. Podosokorskaya, Olga A. Kublanov, Ilya V. Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum |
topic_facet |
Short Genome Report |
description |
A strain of a hyperthermophilic filamentous archaeon was isolated from a sample of Kamchatka hot spring sediment. Isolate 1807-2 grew optimally at 85 °C, pH 6.0-6.5, the parameters being close to those at the sampling site. 16S rRNA gene sequence analysis placed the novel isolate in the crenarchaeal genus Thermofilum; Thermofilum pendens was its closest valid relative (95.7 % of sequence identity). Strain 1807-2 grew organothrophically using polysaccharides (starch and glucomannan), yeast extract or peptone as substrates. The addition of other crenarchaea culture broth filtrates was obligatory required for growth and could not be replaced by the addition of these organisms’ cell wall fractions, as it was described for T. pendens. The genome of strain 1807-2 was sequenced using Illumina and PGM technologies. The average nucleotide identities between genome of strain 1807-2 and T. pendens strain HRK 5T and “T. adornatus” strain 1910b were 85 and 82 %, respectively. On the basis of 16S rRNA gene sequence phylogeny, ANI calculations and phenotypic differences we propose a novel species Thermofilum uzonense with the type strain 1807-2T (= DSM 28062T = JCM 19810T). Project information and genome sequence was deposited in Genbank under IDs PRJNA262459 and CP009961, respectively. |
format |
Text |
author |
Toshchakov, Stepan V. Korzhenkov, Aleksei A. Samarov, Nazar I. Mazunin, Ilia O. Mozhey, Oleg I. Shmyr, Ilya S. Derbikova, Ksenia S. Taranov, Evgeny A. Dominova, Irina N. Bonch-Osmolovskaya, Elizaveta A. Patrushev, Maxim V. Podosokorskaya, Olga A. Kublanov, Ilya V. |
author_facet |
Toshchakov, Stepan V. Korzhenkov, Aleksei A. Samarov, Nazar I. Mazunin, Ilia O. Mozhey, Oleg I. Shmyr, Ilya S. Derbikova, Ksenia S. Taranov, Evgeny A. Dominova, Irina N. Bonch-Osmolovskaya, Elizaveta A. Patrushev, Maxim V. Podosokorskaya, Olga A. Kublanov, Ilya V. |
author_sort |
Toshchakov, Stepan V. |
title |
Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum |
title_short |
Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum |
title_full |
Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum |
title_fullStr |
Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum |
title_full_unstemmed |
Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum |
title_sort |
complete genome sequence of and proposal of thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus thermofilum |
publisher |
BioMed Central |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673724/ https://doi.org/10.1186/s40793-015-0105-y |
genre |
Kamchatka |
genre_facet |
Kamchatka |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673724/ http://dx.doi.org/10.1186/s40793-015-0105-y |
op_rights |
© Toshchakov et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
op_rightsnorm |
CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s40793-015-0105-y |
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