Characterisation of the human embryonic and foetal epicardium during heart development
The epicardium is essential for mammalian heart development. At present, our understanding of the timing and morphogenetic events leading to the formation of the human epicardium has essentially been extrapolated from model organisms. Here, we studied primary tissue samples to characterise human epi...
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ftpubmed:oai:pubmedcentral.nih.gov:4647216 2023-05-15T15:34:40+02:00 Characterisation of the human embryonic and foetal epicardium during heart development Risebro, Catherine A. Vieira, Joaquim Miguel Klotz, Linda Riley, Paul R. 2015-11-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647216/ http://www.ncbi.nlm.nih.gov/pubmed/26395486 https://doi.org/10.1242/dev.127621 en eng The Company of Biologists http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647216/ http://www.ncbi.nlm.nih.gov/pubmed/26395486 http://dx.doi.org/10.1242/dev.127621 © 2015. Published by The Company of Biologists Ltd Stem Cells and Regeneration Text 2015 ftpubmed https://doi.org/10.1242/dev.127621 2016-05-08T00:15:25Z The epicardium is essential for mammalian heart development. At present, our understanding of the timing and morphogenetic events leading to the formation of the human epicardium has essentially been extrapolated from model organisms. Here, we studied primary tissue samples to characterise human epicardium development. We reveal that the epicardium begins to envelop the myocardial surface at Carnegie stage (CS) 11 and this process is completed by CS15, earlier than previously inferred from avian studies. Contrary to prevailing dogma, the formed human epicardium is not a simple squamous epithelium and we reveal evidence of more complex structure, including novel spatial differences aligned to the developing chambers. Specifically, the ventricular, but not atrial, epicardium exhibited areas of expanded epithelium, preferential cell alignment and spindle-like morphology. Likewise, we reveal distinct properties ex vivo, such that ventricular cells spontaneously differentiate and lose epicardial identity, whereas atrial-derived cells remained ‘epithelial-like’. These data provide insight into the developing human epicardium that may contribute to our understanding of congenital heart disease and have implications for the development of strategies for endogenous cell-based cardiac repair. Text Avian Studies PubMed Central (PMC) Development |
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English |
topic |
Stem Cells and Regeneration |
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Stem Cells and Regeneration Risebro, Catherine A. Vieira, Joaquim Miguel Klotz, Linda Riley, Paul R. Characterisation of the human embryonic and foetal epicardium during heart development |
topic_facet |
Stem Cells and Regeneration |
description |
The epicardium is essential for mammalian heart development. At present, our understanding of the timing and morphogenetic events leading to the formation of the human epicardium has essentially been extrapolated from model organisms. Here, we studied primary tissue samples to characterise human epicardium development. We reveal that the epicardium begins to envelop the myocardial surface at Carnegie stage (CS) 11 and this process is completed by CS15, earlier than previously inferred from avian studies. Contrary to prevailing dogma, the formed human epicardium is not a simple squamous epithelium and we reveal evidence of more complex structure, including novel spatial differences aligned to the developing chambers. Specifically, the ventricular, but not atrial, epicardium exhibited areas of expanded epithelium, preferential cell alignment and spindle-like morphology. Likewise, we reveal distinct properties ex vivo, such that ventricular cells spontaneously differentiate and lose epicardial identity, whereas atrial-derived cells remained ‘epithelial-like’. These data provide insight into the developing human epicardium that may contribute to our understanding of congenital heart disease and have implications for the development of strategies for endogenous cell-based cardiac repair. |
format |
Text |
author |
Risebro, Catherine A. Vieira, Joaquim Miguel Klotz, Linda Riley, Paul R. |
author_facet |
Risebro, Catherine A. Vieira, Joaquim Miguel Klotz, Linda Riley, Paul R. |
author_sort |
Risebro, Catherine A. |
title |
Characterisation of the human embryonic and foetal epicardium during heart development |
title_short |
Characterisation of the human embryonic and foetal epicardium during heart development |
title_full |
Characterisation of the human embryonic and foetal epicardium during heart development |
title_fullStr |
Characterisation of the human embryonic and foetal epicardium during heart development |
title_full_unstemmed |
Characterisation of the human embryonic and foetal epicardium during heart development |
title_sort |
characterisation of the human embryonic and foetal epicardium during heart development |
publisher |
The Company of Biologists |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647216/ http://www.ncbi.nlm.nih.gov/pubmed/26395486 https://doi.org/10.1242/dev.127621 |
genre |
Avian Studies |
genre_facet |
Avian Studies |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647216/ http://www.ncbi.nlm.nih.gov/pubmed/26395486 http://dx.doi.org/10.1242/dev.127621 |
op_rights |
© 2015. Published by The Company of Biologists Ltd |
op_doi |
https://doi.org/10.1242/dev.127621 |
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Development |
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1766364982552821760 |