Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents
Two novel polyomaviruses (PyVs) were identified in kidney and chest-cavity fluid samples of wild bank voles (Myodes glareolus) and common voles (Microtus arvalis) collected in Germany. All cloned and sequenced genomes had the typical PyV genome organization, including putative open reading frames fo...
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ftpubmed:oai:pubmedcentral.nih.gov:4608572 2023-05-15T15:56:37+02:00 Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents Nainys, Juozas Timinskas, Albertas Schneider, Julia Ulrich, Rainer G. Gedvilaite, Alma 2015-10-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608572/ http://www.ncbi.nlm.nih.gov/pubmed/26474048 https://doi.org/10.1371/journal.pone.0140916 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608572/ http://www.ncbi.nlm.nih.gov/pubmed/26474048 http://dx.doi.org/10.1371/journal.pone.0140916 http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited CC-BY Research Article Text 2015 ftpubmed https://doi.org/10.1371/journal.pone.0140916 2015-11-01T01:17:14Z Two novel polyomaviruses (PyVs) were identified in kidney and chest-cavity fluid samples of wild bank voles (Myodes glareolus) and common voles (Microtus arvalis) collected in Germany. All cloned and sequenced genomes had the typical PyV genome organization, including putative open reading frames for early regulatory proteins large T antigen and small T antigen on one strand and for structural late proteins (VP1, VP2 and VP3) on the other strand. Virus-like particles (VLPs) were generated by yeast expression of the VP1 protein of both PyVs. VLP-based ELISA and large T-antigen sequence-targeted polymerase-chain reaction investigations demonstrated signs of infection of these novel PyVs in about 42% of bank voles and 18% of common voles. In most cases only viral DNA, but not VP1-specific antibodies were detected. In additional animals exclusively VP1-specific antibodies, but no viral DNA was detected, indicative for virus clearance. Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus. The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively. In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses. Text Common vole Microtus arvalis PubMed Central (PMC) PLOS ONE 10 10 e0140916 |
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Research Article Nainys, Juozas Timinskas, Albertas Schneider, Julia Ulrich, Rainer G. Gedvilaite, Alma Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents |
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description |
Two novel polyomaviruses (PyVs) were identified in kidney and chest-cavity fluid samples of wild bank voles (Myodes glareolus) and common voles (Microtus arvalis) collected in Germany. All cloned and sequenced genomes had the typical PyV genome organization, including putative open reading frames for early regulatory proteins large T antigen and small T antigen on one strand and for structural late proteins (VP1, VP2 and VP3) on the other strand. Virus-like particles (VLPs) were generated by yeast expression of the VP1 protein of both PyVs. VLP-based ELISA and large T-antigen sequence-targeted polymerase-chain reaction investigations demonstrated signs of infection of these novel PyVs in about 42% of bank voles and 18% of common voles. In most cases only viral DNA, but not VP1-specific antibodies were detected. In additional animals exclusively VP1-specific antibodies, but no viral DNA was detected, indicative for virus clearance. Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus. The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively. In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses. |
format |
Text |
author |
Nainys, Juozas Timinskas, Albertas Schneider, Julia Ulrich, Rainer G. Gedvilaite, Alma |
author_facet |
Nainys, Juozas Timinskas, Albertas Schneider, Julia Ulrich, Rainer G. Gedvilaite, Alma |
author_sort |
Nainys, Juozas |
title |
Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents |
title_short |
Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents |
title_full |
Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents |
title_fullStr |
Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents |
title_full_unstemmed |
Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents |
title_sort |
identification of two novel members of the tentative genus wukipolyomavirus in wild rodents |
publisher |
Public Library of Science |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608572/ http://www.ncbi.nlm.nih.gov/pubmed/26474048 https://doi.org/10.1371/journal.pone.0140916 |
genre |
Common vole Microtus arvalis |
genre_facet |
Common vole Microtus arvalis |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608572/ http://www.ncbi.nlm.nih.gov/pubmed/26474048 http://dx.doi.org/10.1371/journal.pone.0140916 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0140916 |
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PLOS ONE |
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10 |
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10 |
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e0140916 |
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