Epithelial Cadherin Determines Resistance to Infectious Pancreatic Necrosis Virus in Atlantic Salmon
Infectious pancreatic necrosis virus (IPNV) is the cause of one of the most prevalent diseases in farmed Atlantic salmon (Salmo salar). A quantitative trait locus (QTL) has been found to be responsible for most of the genetic variation in resistance to the virus. Here we describe how a linkage diseq...
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ftpubmed:oai:pubmedcentral.nih.gov:4574245 2023-05-15T15:31:39+02:00 Epithelial Cadherin Determines Resistance to Infectious Pancreatic Necrosis Virus in Atlantic Salmon Moen, Thomas Torgersen, Jacob Santi, Nina Davidson, William S. Baranski, Matthew Ødegård, Jørgen Kjøglum, Sissel Velle, Bente Kent, Matthew Lubieniecki, Krzysztof P. Isdal, Eivind Lien, Sigbjørn 2015-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574245/ http://www.ncbi.nlm.nih.gov/pubmed/26041276 https://doi.org/10.1534/genetics.115.175406 en eng Genetics Society of America http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574245/ http://www.ncbi.nlm.nih.gov/pubmed/26041276 http://dx.doi.org/10.1534/genetics.115.175406 Copyright © 2015 by the Genetics Society of America Investigations Text 2015 ftpubmed https://doi.org/10.1534/genetics.115.175406 2016-08-07T00:10:29Z Infectious pancreatic necrosis virus (IPNV) is the cause of one of the most prevalent diseases in farmed Atlantic salmon (Salmo salar). A quantitative trait locus (QTL) has been found to be responsible for most of the genetic variation in resistance to the virus. Here we describe how a linkage disequilibrium-based test for deducing the QTL allele was developed, and how it was used to produce IPN-resistant salmon, leading to a 75% decrease in the number of IPN outbreaks in the salmon farming industry. Furthermore, we describe how whole-genome sequencing of individuals with deduced QTL genotypes was used to map the QTL down to a region containing an epithelial cadherin (cdh1) gene. In a coimmunoprecipitation assay, the Cdh1 protein was found to bind to IPNV virions, strongly indicating that the protein is part of the machinery used by the virus for internalization. Immunofluorescence revealed that the virus colocalizes with IPNV in the endosomes of homozygous susceptible individuals but not in the endosomes of homozygous resistant individuals. A putative causal single nucleotide polymorphism was found within the full-length cdh1 gene, in phase with the QTL in all observed haplotypes except one; the absence of a single, all-explaining DNA polymorphism indicates that an additional causative polymorphism may contribute to the observed QTL genotype patterns. Cdh1 has earlier been shown to be necessary for the internalization of certain bacteria and fungi, but this is the first time the protein is implicated in internalization of a virus. Text Atlantic salmon Salmo salar PubMed Central (PMC) Genetics 200 4 1313 1326 |
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Investigations Moen, Thomas Torgersen, Jacob Santi, Nina Davidson, William S. Baranski, Matthew Ødegård, Jørgen Kjøglum, Sissel Velle, Bente Kent, Matthew Lubieniecki, Krzysztof P. Isdal, Eivind Lien, Sigbjørn Epithelial Cadherin Determines Resistance to Infectious Pancreatic Necrosis Virus in Atlantic Salmon |
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Investigations |
description |
Infectious pancreatic necrosis virus (IPNV) is the cause of one of the most prevalent diseases in farmed Atlantic salmon (Salmo salar). A quantitative trait locus (QTL) has been found to be responsible for most of the genetic variation in resistance to the virus. Here we describe how a linkage disequilibrium-based test for deducing the QTL allele was developed, and how it was used to produce IPN-resistant salmon, leading to a 75% decrease in the number of IPN outbreaks in the salmon farming industry. Furthermore, we describe how whole-genome sequencing of individuals with deduced QTL genotypes was used to map the QTL down to a region containing an epithelial cadherin (cdh1) gene. In a coimmunoprecipitation assay, the Cdh1 protein was found to bind to IPNV virions, strongly indicating that the protein is part of the machinery used by the virus for internalization. Immunofluorescence revealed that the virus colocalizes with IPNV in the endosomes of homozygous susceptible individuals but not in the endosomes of homozygous resistant individuals. A putative causal single nucleotide polymorphism was found within the full-length cdh1 gene, in phase with the QTL in all observed haplotypes except one; the absence of a single, all-explaining DNA polymorphism indicates that an additional causative polymorphism may contribute to the observed QTL genotype patterns. Cdh1 has earlier been shown to be necessary for the internalization of certain bacteria and fungi, but this is the first time the protein is implicated in internalization of a virus. |
format |
Text |
author |
Moen, Thomas Torgersen, Jacob Santi, Nina Davidson, William S. Baranski, Matthew Ødegård, Jørgen Kjøglum, Sissel Velle, Bente Kent, Matthew Lubieniecki, Krzysztof P. Isdal, Eivind Lien, Sigbjørn |
author_facet |
Moen, Thomas Torgersen, Jacob Santi, Nina Davidson, William S. Baranski, Matthew Ødegård, Jørgen Kjøglum, Sissel Velle, Bente Kent, Matthew Lubieniecki, Krzysztof P. Isdal, Eivind Lien, Sigbjørn |
author_sort |
Moen, Thomas |
title |
Epithelial Cadherin Determines Resistance to Infectious Pancreatic Necrosis Virus in Atlantic Salmon |
title_short |
Epithelial Cadherin Determines Resistance to Infectious Pancreatic Necrosis Virus in Atlantic Salmon |
title_full |
Epithelial Cadherin Determines Resistance to Infectious Pancreatic Necrosis Virus in Atlantic Salmon |
title_fullStr |
Epithelial Cadherin Determines Resistance to Infectious Pancreatic Necrosis Virus in Atlantic Salmon |
title_full_unstemmed |
Epithelial Cadherin Determines Resistance to Infectious Pancreatic Necrosis Virus in Atlantic Salmon |
title_sort |
epithelial cadherin determines resistance to infectious pancreatic necrosis virus in atlantic salmon |
publisher |
Genetics Society of America |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574245/ http://www.ncbi.nlm.nih.gov/pubmed/26041276 https://doi.org/10.1534/genetics.115.175406 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574245/ http://www.ncbi.nlm.nih.gov/pubmed/26041276 http://dx.doi.org/10.1534/genetics.115.175406 |
op_rights |
Copyright © 2015 by the Genetics Society of America |
op_doi |
https://doi.org/10.1534/genetics.115.175406 |
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Genetics |
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200 |
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4 |
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1313 |
op_container_end_page |
1326 |
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1766362176762675200 |