Precursor cells from Atlantic salmon (Salmo salar) visceral fat holds the plasticity to differentiate into the osteogenic lineage
In order to study the potential plasticity of Atlantic salmon (Salmo salar) precursor cells (aSPCs) from the adipogenic mesenchyme cell lineage to differentiate to the osteogenic lineage, aSPCs were isolated and cultivated under either osteogenic or adipogenic promoting conditions. The results stren...
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ftpubmed:oai:pubmedcentral.nih.gov:4571100 2023-05-15T15:30:56+02:00 Precursor cells from Atlantic salmon (Salmo salar) visceral fat holds the plasticity to differentiate into the osteogenic lineage Ytteborg, Elisabeth Todorcevic, Marijana Krasnov, Aleksei Takle, Harald Kristiansen, Inger Øien Ruyter, Bente 2015-05-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571100/ http://www.ncbi.nlm.nih.gov/pubmed/25948755 https://doi.org/10.1242/bio.201411338 en eng The Company of Biologists http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571100/ http://www.ncbi.nlm.nih.gov/pubmed/25948755 http://dx.doi.org/10.1242/bio.201411338 © 2015. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. CC-BY Research Article Text 2015 ftpubmed https://doi.org/10.1242/bio.201411338 2015-09-20T00:15:33Z In order to study the potential plasticity of Atlantic salmon (Salmo salar) precursor cells (aSPCs) from the adipogenic mesenchyme cell lineage to differentiate to the osteogenic lineage, aSPCs were isolated and cultivated under either osteogenic or adipogenic promoting conditions. The results strengthen the hypothesis that aSPCs most likely are predestined to the adipogenic lineage, but they also hold the flexibility to turn into other lineages given the right stimuli. This assumption is supported by the fact that the transcription factor pparγ , important for regulation of adiopogenesis, was silent in aSPCs grown in osteogenic media, while runx2, important for osteogenic differentiation, was not expressed in aSPCs cultivated in adipogenic media. After 2 weeks in osteogenic promoting conditions the cells started to deposit extracellular matrix and after 4 weeks, the cells started mineralizing secreted matrix. Microarray analyses revealed large-scale transcriptome responses to osteogenic medium after 2 days, changes remained stable at day 15 and decreased by magnitude at day 30. Induction was observed in many genes involved in osteogenic differentiation, growth factors, regulators of development, transporters and production of extracellular matrix. Transcriptome profile in differentiating adipocytes was markedly different from differentiating osteoblasts with far fewer genes changing activity. The number of regulated genes slowly increased at the mature stage, when adipocytes increased in size and accumulated lipids. This is the first report on in vitro differentiation of aSPCs from Atlantic salmon to mineralizing osteogenic cells. This cell model system provides a new valuable tool for studying osteoblastogenesis in fish. Text Atlantic salmon Salmo salar PubMed Central (PMC) Biology Open 4 7 783 791 |
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Research Article Ytteborg, Elisabeth Todorcevic, Marijana Krasnov, Aleksei Takle, Harald Kristiansen, Inger Øien Ruyter, Bente Precursor cells from Atlantic salmon (Salmo salar) visceral fat holds the plasticity to differentiate into the osteogenic lineage |
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Research Article |
description |
In order to study the potential plasticity of Atlantic salmon (Salmo salar) precursor cells (aSPCs) from the adipogenic mesenchyme cell lineage to differentiate to the osteogenic lineage, aSPCs were isolated and cultivated under either osteogenic or adipogenic promoting conditions. The results strengthen the hypothesis that aSPCs most likely are predestined to the adipogenic lineage, but they also hold the flexibility to turn into other lineages given the right stimuli. This assumption is supported by the fact that the transcription factor pparγ , important for regulation of adiopogenesis, was silent in aSPCs grown in osteogenic media, while runx2, important for osteogenic differentiation, was not expressed in aSPCs cultivated in adipogenic media. After 2 weeks in osteogenic promoting conditions the cells started to deposit extracellular matrix and after 4 weeks, the cells started mineralizing secreted matrix. Microarray analyses revealed large-scale transcriptome responses to osteogenic medium after 2 days, changes remained stable at day 15 and decreased by magnitude at day 30. Induction was observed in many genes involved in osteogenic differentiation, growth factors, regulators of development, transporters and production of extracellular matrix. Transcriptome profile in differentiating adipocytes was markedly different from differentiating osteoblasts with far fewer genes changing activity. The number of regulated genes slowly increased at the mature stage, when adipocytes increased in size and accumulated lipids. This is the first report on in vitro differentiation of aSPCs from Atlantic salmon to mineralizing osteogenic cells. This cell model system provides a new valuable tool for studying osteoblastogenesis in fish. |
format |
Text |
author |
Ytteborg, Elisabeth Todorcevic, Marijana Krasnov, Aleksei Takle, Harald Kristiansen, Inger Øien Ruyter, Bente |
author_facet |
Ytteborg, Elisabeth Todorcevic, Marijana Krasnov, Aleksei Takle, Harald Kristiansen, Inger Øien Ruyter, Bente |
author_sort |
Ytteborg, Elisabeth |
title |
Precursor cells from Atlantic salmon (Salmo salar) visceral fat holds the plasticity to differentiate into the osteogenic lineage |
title_short |
Precursor cells from Atlantic salmon (Salmo salar) visceral fat holds the plasticity to differentiate into the osteogenic lineage |
title_full |
Precursor cells from Atlantic salmon (Salmo salar) visceral fat holds the plasticity to differentiate into the osteogenic lineage |
title_fullStr |
Precursor cells from Atlantic salmon (Salmo salar) visceral fat holds the plasticity to differentiate into the osteogenic lineage |
title_full_unstemmed |
Precursor cells from Atlantic salmon (Salmo salar) visceral fat holds the plasticity to differentiate into the osteogenic lineage |
title_sort |
precursor cells from atlantic salmon (salmo salar) visceral fat holds the plasticity to differentiate into the osteogenic lineage |
publisher |
The Company of Biologists |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571100/ http://www.ncbi.nlm.nih.gov/pubmed/25948755 https://doi.org/10.1242/bio.201411338 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571100/ http://www.ncbi.nlm.nih.gov/pubmed/25948755 http://dx.doi.org/10.1242/bio.201411338 |
op_rights |
© 2015. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1242/bio.201411338 |
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