Piscine orthoreovirus (PRV) in red and melanised foci in white muscle of Atlantic salmon (Salmo salar)
Melanised focal changes (black spots) are common findings in the white skeletal muscle of seawater-farmed Atlantic salmon (Salmo salar). Fillets with melanised focal changes are considered as lower quality and cause large economic losses. It has been suggested that red focal changes (red spots) prec...
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ftpubmed:oai:pubmedcentral.nih.gov:4562189 2023-05-15T15:30:46+02:00 Piscine orthoreovirus (PRV) in red and melanised foci in white muscle of Atlantic salmon (Salmo salar) Bjørgen, Håvard Wessel, Øystein Fjelldal, Per Gunnar Hansen, Tom Sveier, Harald Sæbø, Håkon Rydland Enger, Katrine Bones Monsen, Eirik Kvellestad, Agnar Rimstad, Espen Koppang, Erling Olaf 2015-09-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562189/ http://www.ncbi.nlm.nih.gov/pubmed/26346256 https://doi.org/10.1186/s13567-015-0244-6 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562189/ http://www.ncbi.nlm.nih.gov/pubmed/26346256 http://dx.doi.org/10.1186/s13567-015-0244-6 © Bjørgen et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. CC0 PDM CC-BY Research Article Text 2015 ftpubmed https://doi.org/10.1186/s13567-015-0244-6 2015-09-13T00:12:27Z Melanised focal changes (black spots) are common findings in the white skeletal muscle of seawater-farmed Atlantic salmon (Salmo salar). Fillets with melanised focal changes are considered as lower quality and cause large economic losses. It has been suggested that red focal changes (red spots) precede the melanised focal changes. In the present work, we examined different populations of captive and wild salmon for the occurrence of both types of changes, which were investigated for the presence of different viruses by immunohistochemistry and RT-qPCR. The occurrence of red or melanised foci varied significantly between the populations, from none in wild fish control group, low prevalence of small foci in fish kept in in-house tanks, to high prevalence of large foci in farm-raised salmon. Large amounts of Piscine orthoreovirus (PRV) antigen were detected in all foci. No other viruses were detected. Red focal changes contained significantly higher levels of PRV RNA than apparently non-affected areas in white muscle of the same individuals. Some changes displayed a transient form between a red and melanised pathotype, indicating a progression from an acute to a chronic manifestation. We conclude that PRV is associated with the focal pathological changes in the white muscle of farmed Atlantic salmon and is a premise for the development of focal melanised changes. Text Atlantic salmon Salmo salar PubMed Central (PMC) Veterinary Research 46 1 |
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Research Article Bjørgen, Håvard Wessel, Øystein Fjelldal, Per Gunnar Hansen, Tom Sveier, Harald Sæbø, Håkon Rydland Enger, Katrine Bones Monsen, Eirik Kvellestad, Agnar Rimstad, Espen Koppang, Erling Olaf Piscine orthoreovirus (PRV) in red and melanised foci in white muscle of Atlantic salmon (Salmo salar) |
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Research Article |
description |
Melanised focal changes (black spots) are common findings in the white skeletal muscle of seawater-farmed Atlantic salmon (Salmo salar). Fillets with melanised focal changes are considered as lower quality and cause large economic losses. It has been suggested that red focal changes (red spots) precede the melanised focal changes. In the present work, we examined different populations of captive and wild salmon for the occurrence of both types of changes, which were investigated for the presence of different viruses by immunohistochemistry and RT-qPCR. The occurrence of red or melanised foci varied significantly between the populations, from none in wild fish control group, low prevalence of small foci in fish kept in in-house tanks, to high prevalence of large foci in farm-raised salmon. Large amounts of Piscine orthoreovirus (PRV) antigen were detected in all foci. No other viruses were detected. Red focal changes contained significantly higher levels of PRV RNA than apparently non-affected areas in white muscle of the same individuals. Some changes displayed a transient form between a red and melanised pathotype, indicating a progression from an acute to a chronic manifestation. We conclude that PRV is associated with the focal pathological changes in the white muscle of farmed Atlantic salmon and is a premise for the development of focal melanised changes. |
format |
Text |
author |
Bjørgen, Håvard Wessel, Øystein Fjelldal, Per Gunnar Hansen, Tom Sveier, Harald Sæbø, Håkon Rydland Enger, Katrine Bones Monsen, Eirik Kvellestad, Agnar Rimstad, Espen Koppang, Erling Olaf |
author_facet |
Bjørgen, Håvard Wessel, Øystein Fjelldal, Per Gunnar Hansen, Tom Sveier, Harald Sæbø, Håkon Rydland Enger, Katrine Bones Monsen, Eirik Kvellestad, Agnar Rimstad, Espen Koppang, Erling Olaf |
author_sort |
Bjørgen, Håvard |
title |
Piscine orthoreovirus (PRV) in red and melanised foci in white muscle of Atlantic salmon (Salmo salar) |
title_short |
Piscine orthoreovirus (PRV) in red and melanised foci in white muscle of Atlantic salmon (Salmo salar) |
title_full |
Piscine orthoreovirus (PRV) in red and melanised foci in white muscle of Atlantic salmon (Salmo salar) |
title_fullStr |
Piscine orthoreovirus (PRV) in red and melanised foci in white muscle of Atlantic salmon (Salmo salar) |
title_full_unstemmed |
Piscine orthoreovirus (PRV) in red and melanised foci in white muscle of Atlantic salmon (Salmo salar) |
title_sort |
piscine orthoreovirus (prv) in red and melanised foci in white muscle of atlantic salmon (salmo salar) |
publisher |
BioMed Central |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562189/ http://www.ncbi.nlm.nih.gov/pubmed/26346256 https://doi.org/10.1186/s13567-015-0244-6 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562189/ http://www.ncbi.nlm.nih.gov/pubmed/26346256 http://dx.doi.org/10.1186/s13567-015-0244-6 |
op_rights |
© Bjørgen et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
op_rightsnorm |
CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s13567-015-0244-6 |
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Veterinary Research |
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46 |
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1 |
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1766361215866503168 |