Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica
Bacteria belonging to the newly classified candidate phylum “Atribacteria” (formerly referred to as “OP9” and “JS1”) are common in anoxic methane-rich sediments. However, the metabolic functions and biogeochemical role of these microorganisms in the subsurface remains unrealized due to the lack of p...
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ftpubmed:oai:pubmedcentral.nih.gov:4549626 2023-05-15T13:49:24+02:00 Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica Carr, Stephanie A. Orcutt, Beth N. Mandernack, Kevin W. Spear, John R. 2015-08-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549626/ https://doi.org/10.3389/fmicb.2015.00872 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549626/ http://dx.doi.org/10.3389/fmicb.2015.00872 Copyright © 2015 Carr, Orcutt, Mandernack and Spear. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2015 ftpubmed https://doi.org/10.3389/fmicb.2015.00872 2015-09-20T00:09:47Z Bacteria belonging to the newly classified candidate phylum “Atribacteria” (formerly referred to as “OP9” and “JS1”) are common in anoxic methane-rich sediments. However, the metabolic functions and biogeochemical role of these microorganisms in the subsurface remains unrealized due to the lack of pure culture representatives. In this study of deep sediment from Antarctica’s Adélie Basin, collected during Expedition 318 of the Integrated Ocean Drilling Program (IODP), Atribacteria-related sequences of the 16S rRNA gene were abundant (up to 51% of the sequences) and steadily increased in relative abundance with depth throughout the methane-rich zones. To better understand the metabolic potential of Atribacteria within this environment, and to compare with phylogenetically distinct Atribacteria from non-deep-sea environments, individual cells were sorted for single cell genomics from sediment collected from 97.41 m below the seafloor from IODP Hole U1357C. As observed for non-marine Atribacteria, a partial single cell genome suggests a heterotrophic metabolism, with Atribacteria potentially producing fermentation products such as acetate, ethanol, and CO2. These products may in turn support methanogens within the sediment microbial community and explain the frequent occurrence of Atribacteria in anoxic methane-rich sediments. This first report of a single cell genome from deep sediment broadens the known diversity within the Atribacteria phylum and highlights the potential role of Atribacteria in carbon cycling in deep sediment. Text Antarc* Antarctica PubMed Central (PMC) Frontiers in Microbiology 6 |
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English |
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Microbiology |
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Microbiology Carr, Stephanie A. Orcutt, Beth N. Mandernack, Kevin W. Spear, John R. Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica |
topic_facet |
Microbiology |
description |
Bacteria belonging to the newly classified candidate phylum “Atribacteria” (formerly referred to as “OP9” and “JS1”) are common in anoxic methane-rich sediments. However, the metabolic functions and biogeochemical role of these microorganisms in the subsurface remains unrealized due to the lack of pure culture representatives. In this study of deep sediment from Antarctica’s Adélie Basin, collected during Expedition 318 of the Integrated Ocean Drilling Program (IODP), Atribacteria-related sequences of the 16S rRNA gene were abundant (up to 51% of the sequences) and steadily increased in relative abundance with depth throughout the methane-rich zones. To better understand the metabolic potential of Atribacteria within this environment, and to compare with phylogenetically distinct Atribacteria from non-deep-sea environments, individual cells were sorted for single cell genomics from sediment collected from 97.41 m below the seafloor from IODP Hole U1357C. As observed for non-marine Atribacteria, a partial single cell genome suggests a heterotrophic metabolism, with Atribacteria potentially producing fermentation products such as acetate, ethanol, and CO2. These products may in turn support methanogens within the sediment microbial community and explain the frequent occurrence of Atribacteria in anoxic methane-rich sediments. This first report of a single cell genome from deep sediment broadens the known diversity within the Atribacteria phylum and highlights the potential role of Atribacteria in carbon cycling in deep sediment. |
format |
Text |
author |
Carr, Stephanie A. Orcutt, Beth N. Mandernack, Kevin W. Spear, John R. |
author_facet |
Carr, Stephanie A. Orcutt, Beth N. Mandernack, Kevin W. Spear, John R. |
author_sort |
Carr, Stephanie A. |
title |
Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica |
title_short |
Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica |
title_full |
Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica |
title_fullStr |
Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica |
title_full_unstemmed |
Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica |
title_sort |
abundant atribacteria in deep marine sediment from the adélie basin, antarctica |
publisher |
Frontiers Media S.A. |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549626/ https://doi.org/10.3389/fmicb.2015.00872 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549626/ http://dx.doi.org/10.3389/fmicb.2015.00872 |
op_rights |
Copyright © 2015 Carr, Orcutt, Mandernack and Spear. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2015.00872 |
container_title |
Frontiers in Microbiology |
container_volume |
6 |
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1766251311837216768 |