Polar freshwater cyanophage S-EIV1 represents a new widespread evolutionary lineage of phages
Cyanobacteria are often the dominant phototrophs in polar freshwater communities; yet, the phages that infect them remain unknown. Here, we present a genomic and morphological characterization of cyanophage S-EIV1 that was isolated from freshwaters on Ellesmere Island (Nunavut, High Arctic Canada),...
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ftpubmed:oai:pubmedcentral.nih.gov:4542036 2023-05-15T15:07:31+02:00 Polar freshwater cyanophage S-EIV1 represents a new widespread evolutionary lineage of phages Chénard, C Chan, A M Vincent, W F Suttle, C A 2015-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542036/ http://www.ncbi.nlm.nih.gov/pubmed/25822482 https://doi.org/10.1038/ismej.2015.24 en eng Nature Publishing Group http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542036/ http://www.ncbi.nlm.nih.gov/pubmed/25822482 http://dx.doi.org/10.1038/ismej.2015.24 Copyright © 2015 International Society for Microbial Ecology Original Article Text 2015 ftpubmed https://doi.org/10.1038/ismej.2015.24 2016-09-04T00:04:49Z Cyanobacteria are often the dominant phototrophs in polar freshwater communities; yet, the phages that infect them remain unknown. Here, we present a genomic and morphological characterization of cyanophage S-EIV1 that was isolated from freshwaters on Ellesmere Island (Nunavut, High Arctic Canada), and which infects the polar Synechococcus sp., strain PCCC-A2c. S-EIV1 represents a newly discovered evolutionary lineage of bacteriophages whose representatives are widespread in aquatic systems. Among the 130 predicted open reading frames (ORFs) there is no recognizable similarity to genes that encode structural proteins other than the large terminase subunit and a distant viral morphogenesis protein, indicating that the genes encoding the structural proteins of S-EIV1 are distinct from other viruses. As well, only 19 predicted coding sequences on the 79 178 bp circularly permuted genome have homology with genes encoding proteins of known function. Although S-EIV1 is divergent from other sequenced phage isolates, it shares synteny with phage genes captured on a fosmid from the deep-chlorophyll maximum in the Mediterranean Sea, as well as with an incision element in the genome of Anabaena variabilis (ATCC 29413). Sequence recruitment of metagenomic data indicates that S-EIV1-like viruses are cosmopolitan and abundant in a wide range of aquatic systems, suggesting they have an important ecological role. Text Arctic Ellesmere Island Nunavut PubMed Central (PMC) Arctic Canada Ellesmere Island Nunavut The ISME Journal 9 9 2046 2058 |
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Original Article Chénard, C Chan, A M Vincent, W F Suttle, C A Polar freshwater cyanophage S-EIV1 represents a new widespread evolutionary lineage of phages |
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Original Article |
description |
Cyanobacteria are often the dominant phototrophs in polar freshwater communities; yet, the phages that infect them remain unknown. Here, we present a genomic and morphological characterization of cyanophage S-EIV1 that was isolated from freshwaters on Ellesmere Island (Nunavut, High Arctic Canada), and which infects the polar Synechococcus sp., strain PCCC-A2c. S-EIV1 represents a newly discovered evolutionary lineage of bacteriophages whose representatives are widespread in aquatic systems. Among the 130 predicted open reading frames (ORFs) there is no recognizable similarity to genes that encode structural proteins other than the large terminase subunit and a distant viral morphogenesis protein, indicating that the genes encoding the structural proteins of S-EIV1 are distinct from other viruses. As well, only 19 predicted coding sequences on the 79 178 bp circularly permuted genome have homology with genes encoding proteins of known function. Although S-EIV1 is divergent from other sequenced phage isolates, it shares synteny with phage genes captured on a fosmid from the deep-chlorophyll maximum in the Mediterranean Sea, as well as with an incision element in the genome of Anabaena variabilis (ATCC 29413). Sequence recruitment of metagenomic data indicates that S-EIV1-like viruses are cosmopolitan and abundant in a wide range of aquatic systems, suggesting they have an important ecological role. |
format |
Text |
author |
Chénard, C Chan, A M Vincent, W F Suttle, C A |
author_facet |
Chénard, C Chan, A M Vincent, W F Suttle, C A |
author_sort |
Chénard, C |
title |
Polar freshwater cyanophage S-EIV1 represents a new widespread evolutionary lineage of phages |
title_short |
Polar freshwater cyanophage S-EIV1 represents a new widespread evolutionary lineage of phages |
title_full |
Polar freshwater cyanophage S-EIV1 represents a new widespread evolutionary lineage of phages |
title_fullStr |
Polar freshwater cyanophage S-EIV1 represents a new widespread evolutionary lineage of phages |
title_full_unstemmed |
Polar freshwater cyanophage S-EIV1 represents a new widespread evolutionary lineage of phages |
title_sort |
polar freshwater cyanophage s-eiv1 represents a new widespread evolutionary lineage of phages |
publisher |
Nature Publishing Group |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542036/ http://www.ncbi.nlm.nih.gov/pubmed/25822482 https://doi.org/10.1038/ismej.2015.24 |
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Arctic Canada Ellesmere Island Nunavut |
geographic_facet |
Arctic Canada Ellesmere Island Nunavut |
genre |
Arctic Ellesmere Island Nunavut |
genre_facet |
Arctic Ellesmere Island Nunavut |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542036/ http://www.ncbi.nlm.nih.gov/pubmed/25822482 http://dx.doi.org/10.1038/ismej.2015.24 |
op_rights |
Copyright © 2015 International Society for Microbial Ecology |
op_doi |
https://doi.org/10.1038/ismej.2015.24 |
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The ISME Journal |
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9 |
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9 |
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2046 |
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2058 |
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