RAD sequencing and genomic simulations resolve hybrid origins within North American Canis
Top predators are disappearing worldwide, significantly changing ecosystems that depend on top-down regulation. Conflict with humans remains the primary roadblock for large carnivore conservation, but for the eastern wolf (Canis lycaon), disagreement over its evolutionary origins presents a signific...
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ftpubmed:oai:pubmedcentral.nih.gov:4528444 2023-05-15T15:50:26+02:00 RAD sequencing and genomic simulations resolve hybrid origins within North American Canis Rutledge, L. Y. Devillard, S. Boone, J. Q. Hohenlohe, P. A. White, B. N. 2015-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528444/ http://www.ncbi.nlm.nih.gov/pubmed/26156129 https://doi.org/10.1098/rsbl.2015.0303 en eng The Royal Society http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528444/ http://www.ncbi.nlm.nih.gov/pubmed/26156129 http://dx.doi.org/10.1098/rsbl.2015.0303 http://creativecommons.org/licenses/by/4.0/ © 2015 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. CC-BY Evolutionary Biology Text 2015 ftpubmed https://doi.org/10.1098/rsbl.2015.0303 2015-08-16T00:07:37Z Top predators are disappearing worldwide, significantly changing ecosystems that depend on top-down regulation. Conflict with humans remains the primary roadblock for large carnivore conservation, but for the eastern wolf (Canis lycaon), disagreement over its evolutionary origins presents a significant barrier to conservation in Canada and has impeded protection for grey wolves (Canis lupus) in the USA. Here, we use 127 235 single-nucleotide polymorphisms (SNPs) identified from restriction-site associated DNA sequencing (RAD-seq) of wolves and coyotes, in combination with genomic simulations, to test hypotheses of hybrid origins of Canis types in eastern North America. A principal components analysis revealed no evidence to support eastern wolves, or any other Canis type, as the product of grey wolf × western coyote hybridization. In contrast, simulations that included eastern wolves as a distinct taxon clarified the hybrid origins of Great Lakes-boreal wolves and eastern coyotes. Our results support the eastern wolf as a distinct genomic cluster in North America and help resolve hybrid origins of Great Lakes wolves and eastern coyotes. The data provide timely information that will shed new light on the debate over wolf conservation in eastern North America. Text Canis lupus PubMed Central (PMC) Canada Biology Letters 11 7 20150303 |
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English |
topic |
Evolutionary Biology |
spellingShingle |
Evolutionary Biology Rutledge, L. Y. Devillard, S. Boone, J. Q. Hohenlohe, P. A. White, B. N. RAD sequencing and genomic simulations resolve hybrid origins within North American Canis |
topic_facet |
Evolutionary Biology |
description |
Top predators are disappearing worldwide, significantly changing ecosystems that depend on top-down regulation. Conflict with humans remains the primary roadblock for large carnivore conservation, but for the eastern wolf (Canis lycaon), disagreement over its evolutionary origins presents a significant barrier to conservation in Canada and has impeded protection for grey wolves (Canis lupus) in the USA. Here, we use 127 235 single-nucleotide polymorphisms (SNPs) identified from restriction-site associated DNA sequencing (RAD-seq) of wolves and coyotes, in combination with genomic simulations, to test hypotheses of hybrid origins of Canis types in eastern North America. A principal components analysis revealed no evidence to support eastern wolves, or any other Canis type, as the product of grey wolf × western coyote hybridization. In contrast, simulations that included eastern wolves as a distinct taxon clarified the hybrid origins of Great Lakes-boreal wolves and eastern coyotes. Our results support the eastern wolf as a distinct genomic cluster in North America and help resolve hybrid origins of Great Lakes wolves and eastern coyotes. The data provide timely information that will shed new light on the debate over wolf conservation in eastern North America. |
format |
Text |
author |
Rutledge, L. Y. Devillard, S. Boone, J. Q. Hohenlohe, P. A. White, B. N. |
author_facet |
Rutledge, L. Y. Devillard, S. Boone, J. Q. Hohenlohe, P. A. White, B. N. |
author_sort |
Rutledge, L. Y. |
title |
RAD sequencing and genomic simulations resolve hybrid origins within North American Canis |
title_short |
RAD sequencing and genomic simulations resolve hybrid origins within North American Canis |
title_full |
RAD sequencing and genomic simulations resolve hybrid origins within North American Canis |
title_fullStr |
RAD sequencing and genomic simulations resolve hybrid origins within North American Canis |
title_full_unstemmed |
RAD sequencing and genomic simulations resolve hybrid origins within North American Canis |
title_sort |
rad sequencing and genomic simulations resolve hybrid origins within north american canis |
publisher |
The Royal Society |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528444/ http://www.ncbi.nlm.nih.gov/pubmed/26156129 https://doi.org/10.1098/rsbl.2015.0303 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528444/ http://www.ncbi.nlm.nih.gov/pubmed/26156129 http://dx.doi.org/10.1098/rsbl.2015.0303 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ © 2015 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1098/rsbl.2015.0303 |
container_title |
Biology Letters |
container_volume |
11 |
container_issue |
7 |
container_start_page |
20150303 |
_version_ |
1766385372024012800 |