Muscle transcriptome response to ACTH administration in a free-ranging marine mammal
While much of our understanding of stress physiology is derived from biomedical studies, little is known about the downstream molecular consequences of adaptive stress responses in free-living animals. We examined molecular effectors of the stress hormones cortisol and aldosterone in the northern el...
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ftpubmed:oai:pubmedcentral.nih.gov:4525076 2023-05-15T16:05:14+02:00 Muscle transcriptome response to ACTH administration in a free-ranging marine mammal Khudyakov, Jane I. Champagne, Cory D. Preeyanon, Likit Ortiz, Rudy M. Crocker, Daniel E. 2015-06-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525076/ http://www.ncbi.nlm.nih.gov/pubmed/26038394 https://doi.org/10.1152/physiolgenomics.00030.2015 en eng American Physiological Society http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525076/ http://www.ncbi.nlm.nih.gov/pubmed/26038394 http://dx.doi.org/10.1152/physiolgenomics.00030.2015 Copyright © 2015 the American Physiological Society General Interest Text 2015 ftpubmed https://doi.org/10.1152/physiolgenomics.00030.2015 2016-08-07T00:07:11Z While much of our understanding of stress physiology is derived from biomedical studies, little is known about the downstream molecular consequences of adaptive stress responses in free-living animals. We examined molecular effectors of the stress hormones cortisol and aldosterone in the northern elephant seal, a free-ranging study system in which extreme physiological challenges and cortisol fluctuations are a routine part of life history. We stimulated the neuroendocrine stress axis by administering exogenous adrenocorticotropic hormone (ACTH) and examined the resultant effects by measuring corticosteroid hormones, metabolites, and gene expression before, during, and following administration. ACTH induced an elevation in cortisol, aldosterone, glucose, and fatty acids within 2 h, with complete recovery observed within 24 h of administration. The global transcriptional response of elephant seal muscle tissue to ACTH was evaluated by transcriptomics and involved upregulation of a highly coordinated network of conserved glucocorticoid (GC) target genes predicted to promote metabolic substrate availability without causing deleterious effects seen in laboratory animals. Transcriptional recovery from ACTH was characterized by downregulation of GC target genes and restoration of cell proliferation, metabolism, and tissue maintenance pathways within 24 h. Differentially expressed genes included several adipokines not previously described in muscle, reflecting unique metabolic physiology in fasting-adapted animals. This study represents one of the first transcriptome analyses of cellular responses to hypothalamic-pituitary-adrenal axis stimulation in a free-living marine mammal and suggests that compensatory, tissue-sparing mechanisms may enable marine mammals to maintain cortisol and aldosterone sensitivity while avoiding deleterious long-term consequences of stress. Text Elephant Seal PubMed Central (PMC) Physiological Genomics 47 8 318 330 |
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General Interest Khudyakov, Jane I. Champagne, Cory D. Preeyanon, Likit Ortiz, Rudy M. Crocker, Daniel E. Muscle transcriptome response to ACTH administration in a free-ranging marine mammal |
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General Interest |
description |
While much of our understanding of stress physiology is derived from biomedical studies, little is known about the downstream molecular consequences of adaptive stress responses in free-living animals. We examined molecular effectors of the stress hormones cortisol and aldosterone in the northern elephant seal, a free-ranging study system in which extreme physiological challenges and cortisol fluctuations are a routine part of life history. We stimulated the neuroendocrine stress axis by administering exogenous adrenocorticotropic hormone (ACTH) and examined the resultant effects by measuring corticosteroid hormones, metabolites, and gene expression before, during, and following administration. ACTH induced an elevation in cortisol, aldosterone, glucose, and fatty acids within 2 h, with complete recovery observed within 24 h of administration. The global transcriptional response of elephant seal muscle tissue to ACTH was evaluated by transcriptomics and involved upregulation of a highly coordinated network of conserved glucocorticoid (GC) target genes predicted to promote metabolic substrate availability without causing deleterious effects seen in laboratory animals. Transcriptional recovery from ACTH was characterized by downregulation of GC target genes and restoration of cell proliferation, metabolism, and tissue maintenance pathways within 24 h. Differentially expressed genes included several adipokines not previously described in muscle, reflecting unique metabolic physiology in fasting-adapted animals. This study represents one of the first transcriptome analyses of cellular responses to hypothalamic-pituitary-adrenal axis stimulation in a free-living marine mammal and suggests that compensatory, tissue-sparing mechanisms may enable marine mammals to maintain cortisol and aldosterone sensitivity while avoiding deleterious long-term consequences of stress. |
format |
Text |
author |
Khudyakov, Jane I. Champagne, Cory D. Preeyanon, Likit Ortiz, Rudy M. Crocker, Daniel E. |
author_facet |
Khudyakov, Jane I. Champagne, Cory D. Preeyanon, Likit Ortiz, Rudy M. Crocker, Daniel E. |
author_sort |
Khudyakov, Jane I. |
title |
Muscle transcriptome response to ACTH administration in a free-ranging marine mammal |
title_short |
Muscle transcriptome response to ACTH administration in a free-ranging marine mammal |
title_full |
Muscle transcriptome response to ACTH administration in a free-ranging marine mammal |
title_fullStr |
Muscle transcriptome response to ACTH administration in a free-ranging marine mammal |
title_full_unstemmed |
Muscle transcriptome response to ACTH administration in a free-ranging marine mammal |
title_sort |
muscle transcriptome response to acth administration in a free-ranging marine mammal |
publisher |
American Physiological Society |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525076/ http://www.ncbi.nlm.nih.gov/pubmed/26038394 https://doi.org/10.1152/physiolgenomics.00030.2015 |
genre |
Elephant Seal |
genre_facet |
Elephant Seal |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525076/ http://www.ncbi.nlm.nih.gov/pubmed/26038394 http://dx.doi.org/10.1152/physiolgenomics.00030.2015 |
op_rights |
Copyright © 2015 the American Physiological Society |
op_doi |
https://doi.org/10.1152/physiolgenomics.00030.2015 |
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Physiological Genomics |
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47 |
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8 |
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318 |
op_container_end_page |
330 |
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1766401138855247872 |