Characterization of moose intestinal glycosphingolipids
As a part of a systematic investigation of the species-specific expression of glycosphingolipids, acid and non-acid glycosphingolipids were isolated from three small intestines and one large intestine of the moose (Alces alces). The glycosphingolipids were characterized by binding of monoclonal anti...
Published in: | Glycoconjugate Journal |
---|---|
Main Authors: | , , , , , , |
Format: | Text |
Language: | English |
Published: |
Springer US
2015
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515253/ http://www.ncbi.nlm.nih.gov/pubmed/26104834 https://doi.org/10.1007/s10719-015-9604-8 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:4515253 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:4515253 2023-05-15T13:13:27+02:00 Characterization of moose intestinal glycosphingolipids Johansson, Miralda Madar Dedic, Benjamin Lundholm, Klara Branzell, Filip Berner Barone, Angela Benktander, John Teneberg, Susann 2015-06-24 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515253/ http://www.ncbi.nlm.nih.gov/pubmed/26104834 https://doi.org/10.1007/s10719-015-9604-8 en eng Springer US http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515253/ http://www.ncbi.nlm.nih.gov/pubmed/26104834 http://dx.doi.org/10.1007/s10719-015-9604-8 © The Author(s) 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. CC-BY Original Article Text 2015 ftpubmed https://doi.org/10.1007/s10719-015-9604-8 2015-08-02T00:07:43Z As a part of a systematic investigation of the species-specific expression of glycosphingolipids, acid and non-acid glycosphingolipids were isolated from three small intestines and one large intestine of the moose (Alces alces). The glycosphingolipids were characterized by binding of monoclonal antibodies, lectins and bacteria in chromatogram binding assays, and by mass spectrometry. The non-acid fractions were complex mixtures, and all had glycosphingolipids belonging to the lacto- and neolactoseries (lactotriaosylceramide, lactotetraosylceramide, neolactotetraosylceramide, Galα3-Lex hexaosylceramide, and lacto-neolactohexaosylceramide), globo-series (globotriaosylceramide and globotetraosylceramide), and isogloboseries (isoglobotriaosylceramide). Penta- and heptaglycosylceramides with terminal Galili determinants were also characterized. Furthermore, glycosphingolipids with terminal blood group O determinants (H triaosylceramide, H type 2 pentaosylceramide, H type 1 penta- and heptaosylceramide) were characterized in two of the moose small intestines, and in the one large intestine, while the third small intestine had glycosphingolipids with terminal blood group A determinants (A tetraosylceramide, A type 1 hexa- and octaosylceramide, A dodecaosylceramide). The acid glycosphingolipid fractions of moose small and large intestine contained sulfatide, and the gangliosides GM3, GD3, GD1a, GD1b, and also NeuGc and NeuAc variants of the Sda ganglioside and the sialyl-globopenta/SSEA-4 ganglioside. In humans, the NeuAc-globopenta/SSEA-4 ganglioside is a marker of embryonic and adult stem cells, and is also expressed in several human cancers. This is the first time sialyl-globopentaosylceramide/SSEA-4 has been characterized in a fully differentiated normal tissue, and also the first time NeuGc-globopentaosylceramide has been characterized. Text Alces alces PubMed Central (PMC) Glycoconjugate Journal 32 6 393 412 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Original Article |
spellingShingle |
Original Article Johansson, Miralda Madar Dedic, Benjamin Lundholm, Klara Branzell, Filip Berner Barone, Angela Benktander, John Teneberg, Susann Characterization of moose intestinal glycosphingolipids |
topic_facet |
Original Article |
description |
As a part of a systematic investigation of the species-specific expression of glycosphingolipids, acid and non-acid glycosphingolipids were isolated from three small intestines and one large intestine of the moose (Alces alces). The glycosphingolipids were characterized by binding of monoclonal antibodies, lectins and bacteria in chromatogram binding assays, and by mass spectrometry. The non-acid fractions were complex mixtures, and all had glycosphingolipids belonging to the lacto- and neolactoseries (lactotriaosylceramide, lactotetraosylceramide, neolactotetraosylceramide, Galα3-Lex hexaosylceramide, and lacto-neolactohexaosylceramide), globo-series (globotriaosylceramide and globotetraosylceramide), and isogloboseries (isoglobotriaosylceramide). Penta- and heptaglycosylceramides with terminal Galili determinants were also characterized. Furthermore, glycosphingolipids with terminal blood group O determinants (H triaosylceramide, H type 2 pentaosylceramide, H type 1 penta- and heptaosylceramide) were characterized in two of the moose small intestines, and in the one large intestine, while the third small intestine had glycosphingolipids with terminal blood group A determinants (A tetraosylceramide, A type 1 hexa- and octaosylceramide, A dodecaosylceramide). The acid glycosphingolipid fractions of moose small and large intestine contained sulfatide, and the gangliosides GM3, GD3, GD1a, GD1b, and also NeuGc and NeuAc variants of the Sda ganglioside and the sialyl-globopenta/SSEA-4 ganglioside. In humans, the NeuAc-globopenta/SSEA-4 ganglioside is a marker of embryonic and adult stem cells, and is also expressed in several human cancers. This is the first time sialyl-globopentaosylceramide/SSEA-4 has been characterized in a fully differentiated normal tissue, and also the first time NeuGc-globopentaosylceramide has been characterized. |
format |
Text |
author |
Johansson, Miralda Madar Dedic, Benjamin Lundholm, Klara Branzell, Filip Berner Barone, Angela Benktander, John Teneberg, Susann |
author_facet |
Johansson, Miralda Madar Dedic, Benjamin Lundholm, Klara Branzell, Filip Berner Barone, Angela Benktander, John Teneberg, Susann |
author_sort |
Johansson, Miralda Madar |
title |
Characterization of moose intestinal glycosphingolipids |
title_short |
Characterization of moose intestinal glycosphingolipids |
title_full |
Characterization of moose intestinal glycosphingolipids |
title_fullStr |
Characterization of moose intestinal glycosphingolipids |
title_full_unstemmed |
Characterization of moose intestinal glycosphingolipids |
title_sort |
characterization of moose intestinal glycosphingolipids |
publisher |
Springer US |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515253/ http://www.ncbi.nlm.nih.gov/pubmed/26104834 https://doi.org/10.1007/s10719-015-9604-8 |
genre |
Alces alces |
genre_facet |
Alces alces |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515253/ http://www.ncbi.nlm.nih.gov/pubmed/26104834 http://dx.doi.org/10.1007/s10719-015-9604-8 |
op_rights |
© The Author(s) 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s10719-015-9604-8 |
container_title |
Glycoconjugate Journal |
container_volume |
32 |
container_issue |
6 |
container_start_page |
393 |
op_container_end_page |
412 |
_version_ |
1766258486778265600 |