Sulphated glycosaminoglycans and proteoglycans in the developing vertebral column of juvenile Atlantic salmon (Salmo salar)
In the present study, the distribution of sulphated glycosaminoglycans (GAGs) in the developing vertebral column of Atlantic salmon (Salmo salar) at 700, 900, 1100 and 1400 d° was examined by light microscopy. The mineralization pattern was outlined by Alizarin red S and soft structures by Alcian bl...
Published in: | Fish Physiology and Biochemistry |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
Springer Netherlands
2015
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4495713/ http://www.ncbi.nlm.nih.gov/pubmed/25963942 https://doi.org/10.1007/s10695-015-0067-4 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:4495713 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:4495713 2023-05-15T15:30:37+02:00 Sulphated glycosaminoglycans and proteoglycans in the developing vertebral column of juvenile Atlantic salmon (Salmo salar) Hannesson, Kirsten O. Ytteborg, Elisabeth Takle, Harald Enersen, Grethe Bæverfjord, Grete Pedersen, Mona E. 2015-05-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4495713/ http://www.ncbi.nlm.nih.gov/pubmed/25963942 https://doi.org/10.1007/s10695-015-0067-4 en eng Springer Netherlands http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4495713/ http://www.ncbi.nlm.nih.gov/pubmed/25963942 http://dx.doi.org/10.1007/s10695-015-0067-4 © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. CC-BY Article Text 2015 ftpubmed https://doi.org/10.1007/s10695-015-0067-4 2015-07-12T00:08:46Z In the present study, the distribution of sulphated glycosaminoglycans (GAGs) in the developing vertebral column of Atlantic salmon (Salmo salar) at 700, 900, 1100 and 1400 d° was examined by light microscopy. The mineralization pattern was outlined by Alizarin red S and soft structures by Alcian blue. The temporal and spatial distribution patterns of different types of GAGs: chondroitin-4-sulphate/dermatan sulphate, chondroitin-6-sulphate, chondroitin-0-sulphate and keratan sulphate were addressed by immunohistochemistry using monoclonal antibodies against the different GAGs. The specific pattern obtained with the different antibodies suggests a unique role of the different GAG types in pattern formation and mineralization. In addition, the distribution of the different GAG types in normal and malformed vertebral columns from 15 g salmon was compared. A changed expression pattern of GAGs was found in the malformed vertebrae, indicating the involvement of these molecules during the pathogenesis. The molecular size of proteoglycans (PGs) in the vertebrae carrying GAGs was analysed with western blotting, and mRNA transcription of the PGs aggrecan, decorin, biglycan, fibromodulin and lumican by real-time qPCR. Our study reveals the importance of GAGs in development of vertebral column also in Atlantic salmon and indicates that a more comprehensive approach is necessary to completely understand the processes involved. Text Atlantic salmon Salmo salar PubMed Central (PMC) Fish Physiology and Biochemistry 41 4 1029 1051 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Hannesson, Kirsten O. Ytteborg, Elisabeth Takle, Harald Enersen, Grethe Bæverfjord, Grete Pedersen, Mona E. Sulphated glycosaminoglycans and proteoglycans in the developing vertebral column of juvenile Atlantic salmon (Salmo salar) |
topic_facet |
Article |
description |
In the present study, the distribution of sulphated glycosaminoglycans (GAGs) in the developing vertebral column of Atlantic salmon (Salmo salar) at 700, 900, 1100 and 1400 d° was examined by light microscopy. The mineralization pattern was outlined by Alizarin red S and soft structures by Alcian blue. The temporal and spatial distribution patterns of different types of GAGs: chondroitin-4-sulphate/dermatan sulphate, chondroitin-6-sulphate, chondroitin-0-sulphate and keratan sulphate were addressed by immunohistochemistry using monoclonal antibodies against the different GAGs. The specific pattern obtained with the different antibodies suggests a unique role of the different GAG types in pattern formation and mineralization. In addition, the distribution of the different GAG types in normal and malformed vertebral columns from 15 g salmon was compared. A changed expression pattern of GAGs was found in the malformed vertebrae, indicating the involvement of these molecules during the pathogenesis. The molecular size of proteoglycans (PGs) in the vertebrae carrying GAGs was analysed with western blotting, and mRNA transcription of the PGs aggrecan, decorin, biglycan, fibromodulin and lumican by real-time qPCR. Our study reveals the importance of GAGs in development of vertebral column also in Atlantic salmon and indicates that a more comprehensive approach is necessary to completely understand the processes involved. |
format |
Text |
author |
Hannesson, Kirsten O. Ytteborg, Elisabeth Takle, Harald Enersen, Grethe Bæverfjord, Grete Pedersen, Mona E. |
author_facet |
Hannesson, Kirsten O. Ytteborg, Elisabeth Takle, Harald Enersen, Grethe Bæverfjord, Grete Pedersen, Mona E. |
author_sort |
Hannesson, Kirsten O. |
title |
Sulphated glycosaminoglycans and proteoglycans in the developing vertebral column of juvenile Atlantic salmon (Salmo salar) |
title_short |
Sulphated glycosaminoglycans and proteoglycans in the developing vertebral column of juvenile Atlantic salmon (Salmo salar) |
title_full |
Sulphated glycosaminoglycans and proteoglycans in the developing vertebral column of juvenile Atlantic salmon (Salmo salar) |
title_fullStr |
Sulphated glycosaminoglycans and proteoglycans in the developing vertebral column of juvenile Atlantic salmon (Salmo salar) |
title_full_unstemmed |
Sulphated glycosaminoglycans and proteoglycans in the developing vertebral column of juvenile Atlantic salmon (Salmo salar) |
title_sort |
sulphated glycosaminoglycans and proteoglycans in the developing vertebral column of juvenile atlantic salmon (salmo salar) |
publisher |
Springer Netherlands |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4495713/ http://www.ncbi.nlm.nih.gov/pubmed/25963942 https://doi.org/10.1007/s10695-015-0067-4 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4495713/ http://www.ncbi.nlm.nih.gov/pubmed/25963942 http://dx.doi.org/10.1007/s10695-015-0067-4 |
op_rights |
© The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s10695-015-0067-4 |
container_title |
Fish Physiology and Biochemistry |
container_volume |
41 |
container_issue |
4 |
container_start_page |
1029 |
op_container_end_page |
1051 |
_version_ |
1766361062991462400 |