Microbial Consortium Associated with the Antarctic Marine Ciliate Euplotes focardii: An Investigation from Genomic Sequences
We report the characterization of the bacterial consortium associated to Euplotes focardii, a strictly psychrophilic marine ciliate that was maintained in laboratory cultures at 4 °C after its first isolation from Terra Nova Bay, in Antarctica. By Illumina genome analyser, we obtained 11,179 contigs...
Published in: | Microbial Ecology |
---|---|
Main Authors: | , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Springer US
2015
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494151/ http://www.ncbi.nlm.nih.gov/pubmed/25704316 https://doi.org/10.1007/s00248-015-0568-9 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:4494151 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:4494151 2023-05-15T14:00:20+02:00 Microbial Consortium Associated with the Antarctic Marine Ciliate Euplotes focardii: An Investigation from Genomic Sequences Pucciarelli, Sandra Devaraj, Raghul Rajan Mancini, Alessio Ballarini, Patrizia Castelli, Michele Schrallhammer, Martina Petroni, Giulio Miceli, Cristina 2015-02-24 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494151/ http://www.ncbi.nlm.nih.gov/pubmed/25704316 https://doi.org/10.1007/s00248-015-0568-9 en eng Springer US http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494151/ http://www.ncbi.nlm.nih.gov/pubmed/25704316 http://dx.doi.org/10.1007/s00248-015-0568-9 © The Author(s) 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. CC-BY Genes and Genomes Text 2015 ftpubmed https://doi.org/10.1007/s00248-015-0568-9 2015-07-12T00:08:01Z We report the characterization of the bacterial consortium associated to Euplotes focardii, a strictly psychrophilic marine ciliate that was maintained in laboratory cultures at 4 °C after its first isolation from Terra Nova Bay, in Antarctica. By Illumina genome analyser, we obtained 11,179 contigs of potential prokaryotic origin and classified them according to the NCBI’s prokaryotic attributes table. The majority of these sequences correspond to either Bacteroidetes (16 %) or Proteobacteria (78 %). The latter were dominated by gamma- (39 %, including sequences related to the pathogenic genus Francisella), and alpha-proteobacterial (30 %) sequences. Analysis of the Pfam domain family and Gene Ontology term variation revealed that the most frequent terms that appear unique to this consortium correspond to proteins involved in “transmembrane transporter activity” and “oxidoreductase activity”. Furthermore, we identified genes that encode for enzymes involved in the catabolism of complex substance for energy reserves. We also characterized members of the transposase and integrase superfamilies, whose role in bacterial evolution is well documented, as well as putative antifreeze proteins. Antibiotic treatments of E. focardii cultures delayed the cell division of the ciliate. To conclude, our results indicate that this consortium is largely represented by bacteria derived from the original Antarctic sample and may contribute to the survival of E. focardii in laboratory condition. Furthermore, our results suggest that these bacteria may have a more general role in E. focardii survival in its natural cold and oxidative environment. Text Antarc* Antarctic Antarctica PubMed Central (PMC) Antarctic Terra Nova Bay The Antarctic Microbial Ecology 70 2 484 497 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Genes and Genomes |
spellingShingle |
Genes and Genomes Pucciarelli, Sandra Devaraj, Raghul Rajan Mancini, Alessio Ballarini, Patrizia Castelli, Michele Schrallhammer, Martina Petroni, Giulio Miceli, Cristina Microbial Consortium Associated with the Antarctic Marine Ciliate Euplotes focardii: An Investigation from Genomic Sequences |
topic_facet |
Genes and Genomes |
description |
We report the characterization of the bacterial consortium associated to Euplotes focardii, a strictly psychrophilic marine ciliate that was maintained in laboratory cultures at 4 °C after its first isolation from Terra Nova Bay, in Antarctica. By Illumina genome analyser, we obtained 11,179 contigs of potential prokaryotic origin and classified them according to the NCBI’s prokaryotic attributes table. The majority of these sequences correspond to either Bacteroidetes (16 %) or Proteobacteria (78 %). The latter were dominated by gamma- (39 %, including sequences related to the pathogenic genus Francisella), and alpha-proteobacterial (30 %) sequences. Analysis of the Pfam domain family and Gene Ontology term variation revealed that the most frequent terms that appear unique to this consortium correspond to proteins involved in “transmembrane transporter activity” and “oxidoreductase activity”. Furthermore, we identified genes that encode for enzymes involved in the catabolism of complex substance for energy reserves. We also characterized members of the transposase and integrase superfamilies, whose role in bacterial evolution is well documented, as well as putative antifreeze proteins. Antibiotic treatments of E. focardii cultures delayed the cell division of the ciliate. To conclude, our results indicate that this consortium is largely represented by bacteria derived from the original Antarctic sample and may contribute to the survival of E. focardii in laboratory condition. Furthermore, our results suggest that these bacteria may have a more general role in E. focardii survival in its natural cold and oxidative environment. |
format |
Text |
author |
Pucciarelli, Sandra Devaraj, Raghul Rajan Mancini, Alessio Ballarini, Patrizia Castelli, Michele Schrallhammer, Martina Petroni, Giulio Miceli, Cristina |
author_facet |
Pucciarelli, Sandra Devaraj, Raghul Rajan Mancini, Alessio Ballarini, Patrizia Castelli, Michele Schrallhammer, Martina Petroni, Giulio Miceli, Cristina |
author_sort |
Pucciarelli, Sandra |
title |
Microbial Consortium Associated with the Antarctic Marine Ciliate Euplotes focardii: An Investigation from Genomic Sequences |
title_short |
Microbial Consortium Associated with the Antarctic Marine Ciliate Euplotes focardii: An Investigation from Genomic Sequences |
title_full |
Microbial Consortium Associated with the Antarctic Marine Ciliate Euplotes focardii: An Investigation from Genomic Sequences |
title_fullStr |
Microbial Consortium Associated with the Antarctic Marine Ciliate Euplotes focardii: An Investigation from Genomic Sequences |
title_full_unstemmed |
Microbial Consortium Associated with the Antarctic Marine Ciliate Euplotes focardii: An Investigation from Genomic Sequences |
title_sort |
microbial consortium associated with the antarctic marine ciliate euplotes focardii: an investigation from genomic sequences |
publisher |
Springer US |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494151/ http://www.ncbi.nlm.nih.gov/pubmed/25704316 https://doi.org/10.1007/s00248-015-0568-9 |
geographic |
Antarctic Terra Nova Bay The Antarctic |
geographic_facet |
Antarctic Terra Nova Bay The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494151/ http://www.ncbi.nlm.nih.gov/pubmed/25704316 http://dx.doi.org/10.1007/s00248-015-0568-9 |
op_rights |
© The Author(s) 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00248-015-0568-9 |
container_title |
Microbial Ecology |
container_volume |
70 |
container_issue |
2 |
container_start_page |
484 |
op_container_end_page |
497 |
_version_ |
1766269381307793408 |