Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry
Microbial degradation of soil organic matter (SOM) is a key process for terrestrial carbon cycling, although the molecular details of these transformations remain unclear. This study reports the application of ultrahigh resolution mass spectrometry to profile the molecular composition of SOM and its...
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ftpubmed:oai:pubmedcentral.nih.gov:4467038 2023-05-15T15:08:35+02:00 Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry Mann, Benjamin F. Chen, Hongmei Herndon, Elizabeth M. Chu, Rosalie K. Tolic, Nikola Portier, Evan F. Roy Chowdhury, Taniya Robinson, Errol W. Callister, Stephen J. Wullschleger, Stan D. Graham, David E. Liang, Liyuan Gu, Baohua 2015-06-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467038/ http://www.ncbi.nlm.nih.gov/pubmed/26068586 https://doi.org/10.1371/journal.pone.0130557 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467038/ http://www.ncbi.nlm.nih.gov/pubmed/26068586 http://dx.doi.org/10.1371/journal.pone.0130557 https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication CC0 PDM Research Article Text 2015 ftpubmed https://doi.org/10.1371/journal.pone.0130557 2015-07-05T00:17:12Z Microbial degradation of soil organic matter (SOM) is a key process for terrestrial carbon cycling, although the molecular details of these transformations remain unclear. This study reports the application of ultrahigh resolution mass spectrometry to profile the molecular composition of SOM and its degradation during a simulated warming experiment. A soil sample, collected near Barrow, Alaska, USA, was subjected to a 40-day incubation under anoxic conditions and analyzed before and after the incubation to determine changes of SOM composition. A CHO index based on molecular C, H, and O data was utilized to codify SOM components according to their observed degradation potentials. Compounds with a CHO index score between –1 and 0 in a water-soluble fraction (WSF) demonstrated high degradation potential, with a highest shift of CHO index occurred in the N-containing group of compounds, while similar stoichiometries in a base-soluble fraction (BSF) did not. Additionally, compared with the classical H:C vs O:C van Krevelen diagram, CHO index allowed for direct visualization of the distribution of heteroatoms such as N in the identified SOM compounds. We demonstrate that CHO index is useful not only in characterizing arctic SOM at the molecular level but also enabling quantitative description of SOM degradation, thereby facilitating incorporation of the high resolution MS datasets to future mechanistic models of SOM degradation and prediction of greenhouse gas emissions. Text Arctic Barrow permafrost Alaska PubMed Central (PMC) Arctic PLOS ONE 10 6 e0130557 |
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PubMed Central (PMC) |
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English |
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Research Article |
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Research Article Mann, Benjamin F. Chen, Hongmei Herndon, Elizabeth M. Chu, Rosalie K. Tolic, Nikola Portier, Evan F. Roy Chowdhury, Taniya Robinson, Errol W. Callister, Stephen J. Wullschleger, Stan D. Graham, David E. Liang, Liyuan Gu, Baohua Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry |
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Research Article |
description |
Microbial degradation of soil organic matter (SOM) is a key process for terrestrial carbon cycling, although the molecular details of these transformations remain unclear. This study reports the application of ultrahigh resolution mass spectrometry to profile the molecular composition of SOM and its degradation during a simulated warming experiment. A soil sample, collected near Barrow, Alaska, USA, was subjected to a 40-day incubation under anoxic conditions and analyzed before and after the incubation to determine changes of SOM composition. A CHO index based on molecular C, H, and O data was utilized to codify SOM components according to their observed degradation potentials. Compounds with a CHO index score between –1 and 0 in a water-soluble fraction (WSF) demonstrated high degradation potential, with a highest shift of CHO index occurred in the N-containing group of compounds, while similar stoichiometries in a base-soluble fraction (BSF) did not. Additionally, compared with the classical H:C vs O:C van Krevelen diagram, CHO index allowed for direct visualization of the distribution of heteroatoms such as N in the identified SOM compounds. We demonstrate that CHO index is useful not only in characterizing arctic SOM at the molecular level but also enabling quantitative description of SOM degradation, thereby facilitating incorporation of the high resolution MS datasets to future mechanistic models of SOM degradation and prediction of greenhouse gas emissions. |
format |
Text |
author |
Mann, Benjamin F. Chen, Hongmei Herndon, Elizabeth M. Chu, Rosalie K. Tolic, Nikola Portier, Evan F. Roy Chowdhury, Taniya Robinson, Errol W. Callister, Stephen J. Wullschleger, Stan D. Graham, David E. Liang, Liyuan Gu, Baohua |
author_facet |
Mann, Benjamin F. Chen, Hongmei Herndon, Elizabeth M. Chu, Rosalie K. Tolic, Nikola Portier, Evan F. Roy Chowdhury, Taniya Robinson, Errol W. Callister, Stephen J. Wullschleger, Stan D. Graham, David E. Liang, Liyuan Gu, Baohua |
author_sort |
Mann, Benjamin F. |
title |
Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry |
title_short |
Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry |
title_full |
Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry |
title_fullStr |
Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry |
title_full_unstemmed |
Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry |
title_sort |
indexing permafrost soil organic matter degradation using high-resolution mass spectrometry |
publisher |
Public Library of Science |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467038/ http://www.ncbi.nlm.nih.gov/pubmed/26068586 https://doi.org/10.1371/journal.pone.0130557 |
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Arctic |
geographic_facet |
Arctic |
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Arctic Barrow permafrost Alaska |
genre_facet |
Arctic Barrow permafrost Alaska |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467038/ http://www.ncbi.nlm.nih.gov/pubmed/26068586 http://dx.doi.org/10.1371/journal.pone.0130557 |
op_rights |
https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication |
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CC0 PDM |
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https://doi.org/10.1371/journal.pone.0130557 |
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PLOS ONE |
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10 |
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